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Questions and Answers
What are the characteristics of a good culture medium?
What are the characteristics of a good culture medium?
- Provides a nutritional substrate and has a suitable pH
- Provides a suitable pH and is kept in a container that allows contamination
- Provides a nutritional substrate and is not sterile
- Provides a nutritional substrate, has a suitable pH, is sterile, and is kept in a container that doesn't allow contamination (correct)
Which types of culture media can be used for bacteria?
Which types of culture media can be used for bacteria?
- Liquid and solid only
- Solid and semisolid only
- Liquid only
- Liquid, solid, and semisolid (correct)
What is the usual media for anaerobic bacteria?
What is the usual media for anaerobic bacteria?
- Culture broth with liver or egg white and nutrient agar
- Meat broth and Veillon medium
- Culture broth with liver or egg white and agar broth or Veillon medium (correct)
- Meat broth and nutrient agar
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Study Notes
- Culture media is important for achieving a bacteriological diagnosis correctly, safely, and promptly.
- A good culture medium must provide nutritional substrate, have a suitable pH, be sterile, and be kept in a container that doesn't allow contamination.
- Culture media can be liquid, solid, or semisolid and can be classified by composition, type of respiratory process of bacteria, and frequency of use.
- Usual media for aerobic bacteria include meat broth and nutrient agar.
- Nutrient agar can be prepared by adding agar fiber to meat broth or as a powder that needs to be reconstituted with distilled water.
- Semi-solid agar is obtained by adding a small amount of agar to broth.
- Usual media for anaerobic bacteria include culture broth with liver or egg white and agar broth or Veillon medium.
- Liver and egg whites contain cysteine that captures environmental oxygen, creating a favorable environment for anaerobic bacteria.
- Veillon medium is a solid culture medium with meat broth, agar fiber, glucose, and potassium nitrate.
- Before inoculation with bacteria, old media is regenerated by boiling for 10 minutes.
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