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Questions and Answers
What is the purpose of fixation in histology?
What is the purpose of fixation in histology?
To preserve tissues permanently in as life-like a state as possible
Who are the individuals responsible for processing tissues and making glass microscopic slides?
Who are the individuals responsible for processing tissues and making glass microscopic slides?
Histotechnologists
What is the role of dehydration in the tissue processing techniques?
What is the role of dehydration in the tissue processing techniques?
To remove the water from the tissues
Why is formaldehyde used as a fixative in tissue processing?
Why is formaldehyde used as a fixative in tissue processing?
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What is the purpose of the process of clearing in tissue preparation?
What is the purpose of the process of clearing in tissue preparation?
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Study Notes
Tissue Processing in Histology
- Fixation is a crucial step in histology that prevents autolysis, preserves tissue morphology, and enhances structural detail.
Individuals Involved in Tissue Processing
- Histotechnologists are responsible for processing tissues and making glass microscopic slides.
Dehydration in Tissue Processing
- Dehydration is a necessary step in tissue processing that removes water from the tissue, allowing for the infiltration of wax and ultimately, the creation of a solid block of tissue.
- Dehydration is typically done using a series of increasing concentrations of ethanol (50%, 70%, 90%, and 100%).
Role of Formaldehyde in Tissue Processing
- Formaldehyde is commonly used as a fixative in tissue processing due to its ability to cross-link proteins, thereby preserving tissue structure and morphology.
- Formaldehyde is also an effective bacteriostatic agent, which helps to prevent tissue degradation.
Clearing in Tissue Preparation
- The purpose of clearing in tissue preparation is to remove the dehydrating agent (e.g., ethanol) and replace it with a solvent that allows for the infiltration of wax (e.g., xylene or toluene).
- Clearing is essential for creating a solid block of tissue that can be sectioned and stained for microscopic examination.
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Description
Test your knowledge of the techniques used to process tissues for microscopic study in histology laboratories. This quiz covers the preparation of cells and tissues, including biopsies, specimens from surgeries, and tissues from autopsies.
