Histology Slide Preparation Quiz

Best Fixatives

• Cytofixatives, such as ethanol and methanol, are optimal for cytology due to their ability to preserve cellular morphology and increasing contrast for microscopic examination.
• Formalin is widely regarded as the best fixative for histopathology, preserving tissue architecture and preventing autolysis.

Aim of Tissue Fixation

• Tissue fixation aims to preserve the structural integrity and morphology of cells and tissues by halting biochemical processes like autolysis and putrefaction.
• Ensures that tissue samples maintain their physiological state for accurate diagnostic analysis.

Step in Tissue Processing

• The step involving the removal of water and infiltration of increasing concentrations of a clearing agent is called "dehydration and clearing," crucial for preparing tissue for embedding in paraffin.

Prevention of Tissue Drop

• The primary aim of preventing tissues from dropping from slides during fixation is to maintain tissue integrity and prevent loss of cellular components for accurate microscopic evaluation.
• Proper fixation techniques ensure that samples adhere effectively to slides, enhancing the reliability of the histological examination.