Histology Lab Quiz
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Questions and Answers

What is the primary role of the light microscope?

  • To manipulate cellular structures for analysis
  • To provide a three-dimensional view of structures
  • To enhance color in specimens
  • To observe objects that are too small for the naked eye (correct)

    • Which staining property primarily differentiates basic dyes from other types of stains?

  • Basic dyes are ineffective for cytoplasmic staining
  • Basic dyes are used exclusively for nuclear stains
  • Basic dyes have a net positive charge and bind to negatively charged components (correct)
  • Basic dyes are net negative and bind to positive components

    • What color does the nucleus typically appear when stained using standard histological stains?

  • Red
  • Green
  • Intensely blue or purple (correct)
  • Yellow

    • How can the cell membrane typically be distinguished in histological preparations?

    <p>It is often indistinguishable in Hematoxylin and Eosin staining</p> Signup and view all the answers

    What effect does using both Hematoxylin and Eosin together have on cellular structures?

    <p>It can limit visibility of certain cellular structures</p> Signup and view all the answers

    What is indicated by the pinkish or reddish appearance of the cytoplasm in histological samples?

    <p>An abundance of basic dyes interacting with the cytoplasm</p> Signup and view all the answers

    Which of the following best describes the components visible through a light microscope?

    <p>Objects that are too small for the naked eye can be seen</p> Signup and view all the answers

    What limitation exists when using histological stains separately instead of together?

    <p>Loss of finer details in cellular features</p> Signup and view all the answers

    What is the significance of the charge of basic dyes in histology?

    <p>Their charge allows binding to negatively charged tissue components</p> Signup and view all the answers

    What is the diameter of red blood cells (RBC) mentioned in the content?

    <p>7.5µm</p> Signup and view all the answers

    Which of the following cells does NOT have a nucleus according to the information provided?

    <p>Platelets</p> Signup and view all the answers

    What characteristic is noted about the Golgi apparatus in the epididymis?

    <p>It consists of two structures separated by connective tissue.</p> Signup and view all the answers

    What type of nuclei surrounds the capillary endothelial cells in lymph nodes?

    <p>Heterochromatic nuclei</p> Signup and view all the answers

    What cellular component is primarily stained in the histological sample of adipose tissue?

    <p>Lipid droplets</p> Signup and view all the answers

    How are the Golgi apparatus and connective tissue arranged in the epididymis?

    <p>The Golgi apparatus is separated from connective tissue.</p> Signup and view all the answers

    What type of materials can be identified as basophilic or PAS-positive?

    <p>Polysaccharides and certain protein structures</p> Signup and view all the answers

    Which staining method specifically targets DNA of cell nuclei?

    <p>Feulgen reaction</p> Signup and view all the answers

    What is the primary utility of Sudan Black stain in histology?

    <p>Diagnosing metabolic diseases involving fat accumulations</p> Signup and view all the answers

    Which staining technique relies on the use of silver salts to visualize certain elements in nervous tissue?

    <p>Metal impregnation techniques</p> Signup and view all the answers

    What color does Picro-Sirius-Hematoxylin stain collagen when used correctly?

    <p>Red</p> Signup and view all the answers

    What can be inferred about the application of Pararosaniline-Toluidine Blue stain?

    <p>It enhances detail in acrylic resin-embedded sections.</p> Signup and view all the answers

    Which type of tissue stains intensely with Sudan Black?

    <p>Hepatocytes</p> Signup and view all the answers

    Which of the following best describes the application of Feulgen reaction?

    <p>It selectively stains DNA in cell nuclei.</p> Signup and view all the answers

    What is the primary coloration of the cytoplasm stained with Picro-Sirius-Hematoxylin?

    <p>Light violet or pink</p> Signup and view all the answers

    Why are hydrophobic structures not well stained with H&E?

    <p>They repel aqueous solutions.</p> Signup and view all the answers

    What does eosin specifically stain in tissue samples?

    <p>Acidophilic structures</p> Signup and view all the answers

    Which cellular component is primarily associated with basophilia?

    <p>Nucleus</p> Signup and view all the answers

    Which of the following does Mason trichrome staining allow for?

    <p>Distinguishing between complex extracellular components</p> Signup and view all the answers

    Which type of cells are highlighted by acidophilia?

    <p>Red blood cells and duct cells</p> Signup and view all the answers

    What color is the stain associated with basophilic structures when using a common histological stain?

    <p>Blue</p> Signup and view all the answers

    What is a common extracellular protein that may exhibit acidophilia?

    <p>Collagen</p> Signup and view all the answers

    In which scenario might trichrome staining be preferred over single-dye applications?

    <p>When detailed distinctions among tissue components are needed</p> Signup and view all the answers

    Which component is typically stained with eosin in cytological preparations?

    <p>Cytoplasmic structures</p> Signup and view all the answers

    Which of the following is true about the staining process using eosin?

    <p>Eosin is a negatively charged dye that stains acidophilic structures</p> Signup and view all the answers

    What characteristic is associated with eosinophilia in tissues?

    <p>Preference for staining certain protein-rich cells</p> Signup and view all the answers

    What type of cells are primarily found in the pancreas?

    <p>Acinar cells</p> Signup and view all the answers

    What does the presence of chromophilic substances indicate about a cell?

    <p>It synthesizes large amounts of protein.</p> Signup and view all the answers

    In a Wright-Giemsa stained smear, which component tends to stain uniformly pink?

    <p>Erythrocytes</p> Signup and view all the answers

    Where are Nissl or chromophilic substances predominantly found within a cell?

    <p>In free ribosomes and rough ER</p> Signup and view all the answers

    What color does the nucleolus stain with a chromophilic stain?

    <p>Blue</p> Signup and view all the answers

    What structure do Purkinje cells primarily interface with in the cerebellum?

    <p>The molecular layer</p> Signup and view all the answers

    Which of the following best describes the apical cytoplasm of acinar cells?

    <p>Contains unstained secretion granules</p> Signup and view all the answers

    What does the term 'polarized cells' refer to in the context of acinar cells?

    <p>Cells with distinct functional regions</p> Signup and view all the answers

    The differential staining of leukocyte granules in a smear indicates what?

    <p>The specific type of leukocyte present</p> Signup and view all the answers

    What component is mainly contained within the chromophilic substances noted in cells?

    <p>RNA</p> Signup and view all the answers

    Study Notes

    The Microscope

    • Instrument used for viewing objects smaller than the human eye can see.
    • Light microscope components include lenses, illuminating source, and eyepiece.

    Histological Stains

    • Stains help differentiate cellular structures and components in tissues.
    • Hematoxylin and Eosin (H&E) commonly used; H&E stains nuclei blue or purple and cytoplasm pinkish/reddish.
    • Basic dyes, such as hematoxylin, bind to negatively charged tissue components.
    • Eosin stains acidophilic structures a pink hue; used for proteins and various cellular elements.

    Staining Types

    • Trichrome Stains: More complex, allow for better distinction among tissue features.
    • Sudan Black: Detects intracellular accumulations of lipids, cholesterol, and phospholipids.
    • Metal Impregnation Techniques: Use silver salts to visualize extracellular matrix fibers and specific nervous tissue elements.
    • Feulgen Reaction: Stains DNA specifically within cell nuclei.

    Staining Patterns

    • Basophilia: Associated with RNA and DNA, visible as deep blue areas in tissues.
    • Acidophilia (Eosinophilia): Found in red blood cells and other protein-rich structures.

    Additional Histological Samples

    • Pancreatic Cells: Acinar cells visible through staining; nuclei with prominent nucleolus indicate active protein synthesis.
    • Purkinje Cells: Located in the cerebellum, noted for chromophilic nucleolus indicating high RNA content.
    • Adipocytes: Store large lipid droplets, appear unstained with H&E.
    • Wright-Giemsa Staining: Used for blood smears; differentiates types of blood cells by staining granules in leukocytes.

    Key Tissues and Structures

    • Trachea: Exhibits both basophilia and acidophilia, aiding in distinguishing tissue composition.
    • Liver and Hepatocytes: Demonstrates cellular structures and chromophilic substances, involved in metabolism.
    • Golgi Apparatus: Found in the epididymis, involved in processing and packaging proteins; also helps identify tissue structures via staining.

    Measurement and Estimates

    • Red Blood Cells (RBC) measure approximately 7.5 µm in diameter, applicable in estimating sizes of other cellular structures.
    • Nuclei generally present in all cells except RBC and platelets.

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    Histology Lab (001) PDF - University of Northern Philippines

    Description

    Test your knowledge on the fundamental concepts of histology and microscope usage in this lab-focused quiz. Explore cellular structures and their visualization techniques to enhance your understanding of histological techniques.

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