Histochemistry and Immunohistochemistry Quiz
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Questions and Answers

Which of the following statements about histochemistry is correct?

  • It is a highly specific interaction between macromolecules and their respective antibodies.
  • It involves the use of chemical reactions between laboratory chemicals and tissue components to visualize specific antigens.
  • It is a method of tissue staining that provides information about the presence and location of intracellular and extracellular macromolecules. (correct)
  • It is a technique used to demonstrate the presence of antibodies in tissues.

    • Which of the following is an example of a commonly performed histochemical technique?

  • Perls' Prussian blue (PPB) reaction (correct)
  • Polymerase chain reaction (PCR)
  • Western blotting
  • Enzyme-linked immunosorbent assay (ELISA)

    • Which of the following statements about immunohistochemistry is correct?

  • It is a technique that involves the use of chemical reactions to visualize specific macromolecules in tissues.
  • It is a technique that does not require the use of antibodies.
  • It is a highly specific interaction between antibodies and their respective antigens. (correct)
  • It is a method used to demonstrate the presence of iron deposits in tissues.

    • Which of the following is a key requirement for an immunohistochemical technique?

    <p>An antibody against the protein or antigen of interest.</p> Signup and view all the answers

    In immunohistochemistry, what is the purpose of incubating a tissue section with an antibody solution?

    <p>To allow the antibody to bind specifically to the target protein, if present, in the tissue section.</p> Signup and view all the answers

    Which of the following techniques allows for the visualization of antibody-antigen interactions at the cellular or tissue level?

    <p>Immunohistochemistry</p> Signup and view all the answers

    What is the primary purpose of tagging antibodies with fluorescent compounds, peroxidase, or alkaline phosphatase?

    <p>To enhance the visibility of the antibodies during histochemical detection</p> Signup and view all the answers

    What is the purpose of using in situ hybridization?

    <p>To identify specific nucleotide sequences in genes or RNA within cells and tissue sections</p> Signup and view all the answers

    What is the main difference between bone marrow aspiration and bone marrow biopsy?

    <p>Aspiration is used to obtain a differential cell count, while biopsy is used to evaluate overall cellularity and detect focal lesions</p> Signup and view all the answers

    What is the purpose of using electron-dense gold particles to tag antibodies?

    <p>To facilitate the detection of the antibodies using transmission electron microscopy (TEM)</p> Signup and view all the answers

    What is the primary purpose of performing additional tests, such as cytogenetics, molecular studies, and flow cytometry, on bone marrow samples?

    <p>To detect the presence of specific proteins or genetic abnormalities associated with diseases</p> Signup and view all the answers

    Histochemistry focuses on studying the interaction between antigens and antibodies within tissue.

    <p>False</p> Signup and view all the answers

    Immunohistochemical techniques always involve the use of antibodies against the protein or antigen being detected.

    <p>True</p> Signup and view all the answers

    The Perls Prussian blue reaction is a common histochemical technique used to detect iron deposits in diseases like Hemochromatosis.

    <p>True</p> Signup and view all the answers

    Immunohistochemistry involves the incubation of a tissue section in a solution containing an antibody against the protein of interest.

    <p>True</p> Signup and view all the answers

    Antibodies secreted by lymphocytes in immunohistochemistry primarily bind to antigens recognized as a normal part of the organism.

    <p>False</p> Signup and view all the answers

    All histochemical techniques rely on the specific interaction between an antigen and its antibody.

    <p>False</p> Signup and view all the answers

    Hybridization always involves the binding of two double strands of nucleic acid.

    <p>False</p> Signup and view all the answers

    Fluorescent compounds are commonly used to tag antibodies in histochemical detection.

    <p>True</p> Signup and view all the answers

    In immunohistochemistry, the purpose of incubating a tissue section with an antibody solution is to visualize the binding between two specific proteins.

    <p>True</p> Signup and view all the answers

    The greater the differences in nucleotide sequences, the more readily the complementary strands form 'hybrid' double-strand molecules.

    <p>False</p> Signup and view all the answers

    Bone marrow aspiration is primarily utilized for assessing the overall cellularity of the marrow.

    <p>False</p> Signup and view all the answers

    Immunohistochemistry can be used by pathologists to diagnose many diseases, including certain types of tumors and some virus-infected cells.

    <p>True</p> Signup and view all the answers

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