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Questions and Answers
Which of the following statements about histochemistry is correct?
Which of the following statements about histochemistry is correct?
- It is a highly specific interaction between macromolecules and their respective antibodies.
- It involves the use of chemical reactions between laboratory chemicals and tissue components to visualize specific antigens.
- It is a method of tissue staining that provides information about the presence and location of intracellular and extracellular macromolecules. (correct)
- It is a technique used to demonstrate the presence of antibodies in tissues.
Which of the following is an example of a commonly performed histochemical technique?
Which of the following is an example of a commonly performed histochemical technique?
- Perls' Prussian blue (PPB) reaction (correct)
- Polymerase chain reaction (PCR)
- Western blotting
- Enzyme-linked immunosorbent assay (ELISA)
Which of the following statements about immunohistochemistry is correct?
Which of the following statements about immunohistochemistry is correct?
- It is a technique that involves the use of chemical reactions to visualize specific macromolecules in tissues.
- It is a technique that does not require the use of antibodies.
- It is a highly specific interaction between antibodies and their respective antigens. (correct)
- It is a method used to demonstrate the presence of iron deposits in tissues.
Which of the following is a key requirement for an immunohistochemical technique?
Which of the following is a key requirement for an immunohistochemical technique?
In immunohistochemistry, what is the purpose of incubating a tissue section with an antibody solution?
In immunohistochemistry, what is the purpose of incubating a tissue section with an antibody solution?
Which of the following techniques allows for the visualization of antibody-antigen interactions at the cellular or tissue level?
Which of the following techniques allows for the visualization of antibody-antigen interactions at the cellular or tissue level?
What is the primary purpose of tagging antibodies with fluorescent compounds, peroxidase, or alkaline phosphatase?
What is the primary purpose of tagging antibodies with fluorescent compounds, peroxidase, or alkaline phosphatase?
What is the purpose of using in situ hybridization?
What is the purpose of using in situ hybridization?
What is the main difference between bone marrow aspiration and bone marrow biopsy?
What is the main difference between bone marrow aspiration and bone marrow biopsy?
What is the purpose of using electron-dense gold particles to tag antibodies?
What is the purpose of using electron-dense gold particles to tag antibodies?
What is the primary purpose of performing additional tests, such as cytogenetics, molecular studies, and flow cytometry, on bone marrow samples?
What is the primary purpose of performing additional tests, such as cytogenetics, molecular studies, and flow cytometry, on bone marrow samples?
Histochemistry focuses on studying the interaction between antigens and antibodies within tissue.
Histochemistry focuses on studying the interaction between antigens and antibodies within tissue.
Immunohistochemical techniques always involve the use of antibodies against the protein or antigen being detected.
Immunohistochemical techniques always involve the use of antibodies against the protein or antigen being detected.
The Perls Prussian blue reaction is a common histochemical technique used to detect iron deposits in diseases like Hemochromatosis.
The Perls Prussian blue reaction is a common histochemical technique used to detect iron deposits in diseases like Hemochromatosis.
Immunohistochemistry involves the incubation of a tissue section in a solution containing an antibody against the protein of interest.
Immunohistochemistry involves the incubation of a tissue section in a solution containing an antibody against the protein of interest.
Antibodies secreted by lymphocytes in immunohistochemistry primarily bind to antigens recognized as a normal part of the organism.
Antibodies secreted by lymphocytes in immunohistochemistry primarily bind to antigens recognized as a normal part of the organism.
All histochemical techniques rely on the specific interaction between an antigen and its antibody.
All histochemical techniques rely on the specific interaction between an antigen and its antibody.
Hybridization always involves the binding of two double strands of nucleic acid.
Hybridization always involves the binding of two double strands of nucleic acid.
Fluorescent compounds are commonly used to tag antibodies in histochemical detection.
Fluorescent compounds are commonly used to tag antibodies in histochemical detection.
In immunohistochemistry, the purpose of incubating a tissue section with an antibody solution is to visualize the binding between two specific proteins.
In immunohistochemistry, the purpose of incubating a tissue section with an antibody solution is to visualize the binding between two specific proteins.
The greater the differences in nucleotide sequences, the more readily the complementary strands form 'hybrid' double-strand molecules.
The greater the differences in nucleotide sequences, the more readily the complementary strands form 'hybrid' double-strand molecules.
Bone marrow aspiration is primarily utilized for assessing the overall cellularity of the marrow.
Bone marrow aspiration is primarily utilized for assessing the overall cellularity of the marrow.
Immunohistochemistry can be used by pathologists to diagnose many diseases, including certain types of tumors and some virus-infected cells.
Immunohistochemistry can be used by pathologists to diagnose many diseases, including certain types of tumors and some virus-infected cells.
