23 Questions
Which of the following statements about histochemistry is correct?
It is a method of tissue staining that provides information about the presence and location of intracellular and extracellular macromolecules.
Which of the following is an example of a commonly performed histochemical technique?
Perls' Prussian blue (PPB) reaction
Which of the following statements about immunohistochemistry is correct?
It is a highly specific interaction between antibodies and their respective antigens.
Which of the following is a key requirement for an immunohistochemical technique?
An antibody against the protein or antigen of interest.
In immunohistochemistry, what is the purpose of incubating a tissue section with an antibody solution?
To allow the antibody to bind specifically to the target protein, if present, in the tissue section.
Which of the following techniques allows for the visualization of antibody-antigen interactions at the cellular or tissue level?
Immunohistochemistry
What is the primary purpose of tagging antibodies with fluorescent compounds, peroxidase, or alkaline phosphatase?
To enhance the visibility of the antibodies during histochemical detection
What is the purpose of using in situ hybridization?
To identify specific nucleotide sequences in genes or RNA within cells and tissue sections
What is the main difference between bone marrow aspiration and bone marrow biopsy?
Aspiration is used to obtain a differential cell count, while biopsy is used to evaluate overall cellularity and detect focal lesions
What is the purpose of using electron-dense gold particles to tag antibodies?
To facilitate the detection of the antibodies using transmission electron microscopy (TEM)
What is the primary purpose of performing additional tests, such as cytogenetics, molecular studies, and flow cytometry, on bone marrow samples?
To detect the presence of specific proteins or genetic abnormalities associated with diseases
Histochemistry focuses on studying the interaction between antigens and antibodies within tissue.
False
Immunohistochemical techniques always involve the use of antibodies against the protein or antigen being detected.
True
The Perls Prussian blue reaction is a common histochemical technique used to detect iron deposits in diseases like Hemochromatosis.
True
Immunohistochemistry involves the incubation of a tissue section in a solution containing an antibody against the protein of interest.
True
Antibodies secreted by lymphocytes in immunohistochemistry primarily bind to antigens recognized as a normal part of the organism.
False
All histochemical techniques rely on the specific interaction between an antigen and its antibody.
False
Hybridization always involves the binding of two double strands of nucleic acid.
False
Fluorescent compounds are commonly used to tag antibodies in histochemical detection.
True
In immunohistochemistry, the purpose of incubating a tissue section with an antibody solution is to visualize the binding between two specific proteins.
True
The greater the differences in nucleotide sequences, the more readily the complementary strands form 'hybrid' double-strand molecules.
False
Bone marrow aspiration is primarily utilized for assessing the overall cellularity of the marrow.
False
Immunohistochemistry can be used by pathologists to diagnose many diseases, including certain types of tumors and some virus-infected cells.
True
Test your knowledge of histochemistry and immunohistochemistry, which involve the use of chemical reactions to study tissue components and macromolecules. Explore techniques like Perls Prussian blue reaction and the highly specific interactions in immunohistochemistry.
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