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Questions and Answers
What is the primary purpose of gel electrophoresis in molecular biology?
What is the primary purpose of gel electrophoresis in molecular biology?
- To synthesize RNA
- To sequence DNA
- To separate DNA fragments by size (correct)
- To amplify DNA sequences
Which of the following gels is most commonly used for DNA separation?
Which of the following gels is most commonly used for DNA separation?
- Starch
- Gelatin
- Agarose (correct)
- Polyacrylamide
What does the stationary phase do in high performance liquid chromatography?
What does the stationary phase do in high performance liquid chromatography?
- Carries the sample through the column
- Separates the sample components based on interactions (correct)
- Detects the sample components
- Moves with the mobile phase
Which of the following is NOT a type of high performance liquid chromatography column?
Which of the following is NOT a type of high performance liquid chromatography column?
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Which detector is commonly used in high performance liquid chromatography for compounds that absorb UV or visible light?
Which detector is commonly used in high performance liquid chromatography for compounds that absorb UV or visible light?
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Which technique is utilized to separate DNA fragments by size?
Which technique is utilized to separate DNA fragments by size?
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Study Notes
High Performance Liquid Chromatography
- High Performance Liquid Chromatography (HPLC) is used to detect sample components.
- Solid-phase is NOT a type of HPLC column.
- The stationary phase in HPLC separates sample components based on interactions.
- The diode array detector is commonly used in HPLC for compounds that absorb UV or visible light.
Gel Electrophoresis
- The primary purpose of gel electrophoresis in molecular biology is to separate DNA fragments by size.
- Agarose gel is most commonly used for DNA separation.
- Polyacrylamide gel is not typically used for DNA separation, but may be used for other purposes.
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