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Questions and Answers
What is the primary purpose of gel electrophoresis in molecular biology?
Which of the following gels is most commonly used for DNA separation?
What does the stationary phase do in high performance liquid chromatography?
Which of the following is NOT a type of high performance liquid chromatography column?
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Which detector is commonly used in high performance liquid chromatography for compounds that absorb UV or visible light?
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Which technique is utilized to separate DNA fragments by size?
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Study Notes
High Performance Liquid Chromatography
- High Performance Liquid Chromatography (HPLC) is used to detect sample components.
- Solid-phase is NOT a type of HPLC column.
- The stationary phase in HPLC separates sample components based on interactions.
- The diode array detector is commonly used in HPLC for compounds that absorb UV or visible light.
Gel Electrophoresis
- The primary purpose of gel electrophoresis in molecular biology is to separate DNA fragments by size.
- Agarose gel is most commonly used for DNA separation.
- Polyacrylamide gel is not typically used for DNA separation, but may be used for other purposes.
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Description
Test your knowledge on the basics of High Performance Liquid Chromatography (HPLC), a separation technique used in chemistry and biology. Ensure you understand the principles, types of columns and stationary phase roles.
