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High Performance Liquid Chromatography Quiz

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High Performance Liquid Chromatography Quiz

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Questions and Answers

What is the primary purpose of gel electrophoresis in molecular biology?

To synthesize RNA

To sequence DNA

To separate DNA fragments by size (correct)

To amplify DNA sequences

Which of the following gels is most commonly used for DNA separation?

Starch

Gelatin

Agarose (correct)

Polyacrylamide

What does the stationary phase do in high performance liquid chromatography?

Carries the sample through the column

Separates the sample components based on interactions (correct)

Detects the sample components

Moves with the mobile phase

Which of the following is NOT a type of high performance liquid chromatography column?

Solid-phase Signup and view all the answers

Which detector is commonly used in high performance liquid chromatography for compounds that absorb UV or visible light?

Refractive index detector Signup and view all the answers

Which technique is utilized to separate DNA fragments by size?

Gel Electrophoresis Signup and view all the answers

Study Notes

High Performance Liquid Chromatography

High Performance Liquid Chromatography (HPLC) is used to detect sample components.
Solid-phase is NOT a type of HPLC column.
The stationary phase in HPLC separates sample components based on interactions.
The diode array detector is commonly used in HPLC for compounds that absorb UV or visible light.

Gel Electrophoresis

The primary purpose of gel electrophoresis in molecular biology is to separate DNA fragments by size.
Agarose gel is most commonly used for DNA separation.
Polyacrylamide gel is not typically used for DNA separation, but may be used for other purposes.

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Description

Test your knowledge on the basics of High Performance Liquid Chromatography (HPLC), a separation technique used in chemistry and biology. Ensure you understand the principles, types of columns and stationary phase roles.