Questions and Answers
Why is it important to clear paraffin embedded sections in xylene before staining?
To remove the paraffin and make the tissue accessible to the stain
What is the primary purpose of staining specimens with H&E in the histology laboratory?
To confirm the basic tissue type and help to localize the lesion
Which step in the staining procedure is responsible for selectively staining the cell nuclei?
Stain with Harris hematoxylin for 5 mins
What is the purpose of the blueing step in the staining procedure?
Signup and view all the answers
Why is it important not to use fixatives containing osmic acid for regressive staining?
Signup and view all the answers
What would be the consequence if the counterstain step with eosin is skipped in the staining procedure?
Signup and view all the answers
What is the active coloring agent of hematoxylin?
Signup and view all the answers
Which mordant is used in Aluminum (Alum) Hematoxylin?
Signup and view all the answers
What substance is added to Ehrlich's Hematoxylin to act as an oxidation stabilizer?
Signup and view all the answers
Which step in the staining procedure is sodium iodate used to hasten the process?
Signup and view all the answers
Which hematoxylin solution smells like wine and has a deep purple-red hue?
Signup and view all the answers
What is the original oxidizer used in Harris Hematoxylin?
Signup and view all the answers
'Mucopolysaccharide substances such as cartilage and cement lines of bones are stained intensely blue' is a characteristic of which hematoxylin solution?
Signup and view all the answers
'Not ideal for frozen section' is a characteristic of which hematoxylin solution?
Signup and view all the answers
What is added to Delafield's Hematoxylin to stabilize the oxidation process?
Signup and view all the answers
What hematoxylin solution is recommended for routine use and especially used in sequence with Celestine blue?
Signup and view all the answers