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Questions and Answers
Why is it important to clear paraffin embedded sections in xylene before staining?
Why is it important to clear paraffin embedded sections in xylene before staining?
What is the primary purpose of staining specimens with H&E in the histology laboratory?
What is the primary purpose of staining specimens with H&E in the histology laboratory?
Which step in the staining procedure is responsible for selectively staining the cell nuclei?
Which step in the staining procedure is responsible for selectively staining the cell nuclei?
What is the purpose of the blueing step in the staining procedure?
What is the purpose of the blueing step in the staining procedure?
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Why is it important not to use fixatives containing osmic acid for regressive staining?
Why is it important not to use fixatives containing osmic acid for regressive staining?
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What would be the consequence if the counterstain step with eosin is skipped in the staining procedure?
What would be the consequence if the counterstain step with eosin is skipped in the staining procedure?
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What is the active coloring agent of hematoxylin?
What is the active coloring agent of hematoxylin?
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Which mordant is used in Aluminum (Alum) Hematoxylin?
Which mordant is used in Aluminum (Alum) Hematoxylin?
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What substance is added to Ehrlich's Hematoxylin to act as an oxidation stabilizer?
What substance is added to Ehrlich's Hematoxylin to act as an oxidation stabilizer?
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Which step in the staining procedure is sodium iodate used to hasten the process?
Which step in the staining procedure is sodium iodate used to hasten the process?
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Which hematoxylin solution smells like wine and has a deep purple-red hue?
Which hematoxylin solution smells like wine and has a deep purple-red hue?
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What is the original oxidizer used in Harris Hematoxylin?
What is the original oxidizer used in Harris Hematoxylin?
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'Mucopolysaccharide substances such as cartilage and cement lines of bones are stained intensely blue' is a characteristic of which hematoxylin solution?
'Mucopolysaccharide substances such as cartilage and cement lines of bones are stained intensely blue' is a characteristic of which hematoxylin solution?
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'Not ideal for frozen section' is a characteristic of which hematoxylin solution?
'Not ideal for frozen section' is a characteristic of which hematoxylin solution?
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What is added to Delafield's Hematoxylin to stabilize the oxidation process?
What is added to Delafield's Hematoxylin to stabilize the oxidation process?
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What hematoxylin solution is recommended for routine use and especially used in sequence with Celestine blue?
What hematoxylin solution is recommended for routine use and especially used in sequence with Celestine blue?
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Study Notes
Importance of Clearing Paraffin Embedded Sections
- Clearing in xylene removes excess paraffin, allowing for effective penetration of staining reagents.
- Ensures proper adhesion of stains to tissue sections by enhancing permeability.
Purpose of Staining Specimens with H&E
- Hematoxylin and Eosin (H&E) staining provides contrast, highlighting cellular structures.
- Hematoxylin stains nuclei blue, while eosin stains cytoplasm and extracellular matrix pink, facilitating tissue evaluation.
Step Responsible for Staining Cell Nuclei
- The hematoxylin staining step specifically targets and stains cell nuclei, making them visible under a microscope.
Purpose of the Blueing Step
- The blueing step involves adjusting the pH of the specimen, optimizing the visibility of hematoxylin-stained nuclei by altering the hue from purple to blue.
Avoiding Fixatives with Osmic Acid
- Osmic acid promotes black precipitate formation, which can interfere with regressive staining protocols, complicating the differentiation of tissue structures.
Consequences of Skipping the Eosin Counterstain
- Omitting eosin results in underrepresented cytoplasmic and connective tissue structures, leading to a less informative overall tissue morphology.
Active Coloring Agent of Hematoxylin
- Hematoxylin itself acts as the primary dye, offering color through its oxidized form, which binds to nuclear components.
Mordant Used in Aluminum Hematoxylin
- Aluminum ions serve as mordants in aluminum hematoxylin, enhancing the binding of hematoxylin to tissue components.
Oxidation Stabilizer in Ehrlich's Hematoxylin
- Sodium iodate is incorporated into Ehrlich's hematoxylin to stabilize oxidation and maintain the dye's effectiveness.
Step Promoted by Sodium Iodate
- Sodium iodate is used to hasten the oxidation step in the staining procedure, crucial for activating hematoxylin.
Harris Hematoxylin Characteristics
- Harris hematoxylin has a wine-like odor and a distinct deep purple-red color, often making it noticeable during staining procedures.
Hematoxylin Solution for Mucopolysaccharides
- The characteristic of staining mucopolysaccharides, such as cartilage and cement lines of bones, intensely blue is associated with Alcian blue.
Not Ideal for Frozen Sections
- Harris hematoxylin is not optimal for frozen section analysis due to its formulation affecting tissue morphology.
Stabilizer in Delafield's Hematoxylin
- A small amount of mercuric chloride is added to Delafield's hematoxylin to stabilize the oxidation process, maintaining the quality of the stain.
Recommended Routine Hematoxylin Solution
- Mayer's hematoxylin is preferred for routine use, especially when used in sequence with Celestine blue for effective nuclear and cytoplasmic staining.
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Description
Test your knowledge of the process and significance of Hematoxylin and Eosin (H&E) staining in histology. Learn about its role in confirming basic tissue type and localizing lesions in specimens.
