Hematology Laboratory Safety Quiz
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National Patient Safety Goals

Objectives to enhance patient safety practices in healthcare.

Laboratory Safety Manual

Document designed to protect patients and staff in a lab setting.

Standard precautions

Infection control measures to prevent exposure to bloodborne pathogens.

Exposure to HIV

Risk of infection depends on various factors including contact type.

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Preanalytical error

Mistakes occurring before analysis of lab specimens.

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Delta check

Comparison of current and past lab results for consistency.

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Control specimen

Known sample used to monitor the accuracy of tests.

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Accuracy in testing

Closeness of test results to the true value.

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Precision in testing

Consistency of test results when repeated.

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QC Program

Quality Control measures to ensure lab testing reliability.

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Standard deviation

Indicator of how much data varies from the average.

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Blood sample collection

Process of obtaining blood for laboratory testing.

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Acidic staining effect

Imparts a lower pH color to blood cells during staining.

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Wright's stain

Type of stain used for examining blood smears.

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Maturation sequence

Stages of development of blood cells.

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Erythropoietin

Hormone stimulating red blood cell production.

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Microcytes

Smaller than normal erythrocytes.

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Macrocytes

Larger than normal erythrocytes.

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Poikilocytosis

Variation in erythrocyte shape.

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Rouleaux formation

Erythrocytes stacking like coins.

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Plasmodium species

Genus of protozoa causing malaria.

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Neutrophils

Most common type of white blood cell.

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Phagocytosis

Process of engulfing and digesting pathogens.

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Lymphocytes

Type of white blood cell involved in immune response.

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Antibody production

Creation of proteins to identify and neutralize pathogens.

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Thymus function

Primary site of T lymphocyte maturation.

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Total leukocyte count

Measurement of total white blood cells in blood.

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Cytokines

Proteins that mediate and regulate immunity and inflammation.

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Hematopoiesis

Process of blood cell formation.

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Study Notes

Hematology Laboratory Review Questions

  • National Patient Safety Goals include the use of patient identifiers, proper collection tubes, proper disinfection of laboratory countertops, and proper donning of latex gloves.
  • The primary purpose of a laboratory safety manual is to protect patients and laboratory personnel, and comply with local, state, and OSHA regulations.
  • If a blood specimen is spilled, immediately wear gloves and a lab coat, absorb the blood with disposable towels, clean with a 1% chlorine solution, and then wash with water.
  • The likelihood of infection after exposure to HBV or HIV depends least on the presence of nonintact skin, and most on the concentration of the virus in the blood and duration of contact.

Standard Precautions

  • Standard precautions prevent healthcare workers from exposure to bloodborne pathogens, including HIV and HBV.
  • Exposure to blood and body fluids is the major cause of HIV and HBV infection in healthcare personnel.
  • A 1:10 dilution of bleach is needed for disinfecting work surfaces.

Quality Assessment

  • The most frequent error in laboratory specimen handling is preanalytical error.
  • The delta check detects discrepancies between current and past patient results.
  • Preanalytical errors include wrong assays, failure to run normal controls during testing, and incorrect specimen labeling.

Quality Control

  • Control specimens are known values similar to patient specimens, used to monitor accuracy.

Accuracy and Precision

  • Accuracy refers to closeness to the true value.
  • Precision refers to how close test results are when repeated.
  • Calibration means comparing to a known physical constant.

Standard Deviation

  • Standard deviation expresses the degree to which test data vary about the average.
  • Variance is the sum of the squared differences from the mean.

Minimum Number of Patient Samples

  • When validating a control reference range, 100 patient samples are needed .
  • Acceptable control values fall within ±3 standard deviations of the mean.
  • Progressive changes occur in one direction from the mean for at least three days.
  • Scattered changes occur in various directions away from the mean over at least one week.

Hematology Lab Safety

  • Safe use of evacuated tubes and correct handling of blood samples.
  • Using correct evacuated tubes and their associated color coded stoppers for different anticoagulants.
  • Correct handling of blood and body fluids in the laboratory.

Venipuncture Procedures (Sequence)

  • Select a site and properly prep the site.
  • Identify the patient, check test requisitions, and assemble equipment.
  • Put on gloves after washing hands.
  • Reapply the tourniquet and perform the venipuncture.
  • Remove tourniquet, withdraw needle, apply pressure, and label tubes correctly.
  • Introduce self to patient and briefly explain procedure.

Appropriate Veins for Venipuncture

  • Cephalic, basilic, and median cubital veins.

Blood Samples: Special Considerations

  • Appropriate procedures when taking blood from a patient with IVs in their arms.
  • Safety around IV lines and proper procedure should be followed at all times; do not disconnect an IV line without proper authorization.

Blood Smears

  • Wiping away excess blood and introducing proper quality blood into the column of blood.

Hematology Instruments / Procedures

  • Using appropriate color-coded evacuated tubes based on the anticoagulant used.

Additional Hematology Information

  • Identifying appropriate sites and types of veins for venipuncture.
  • Proper handling, labeling, and storage of blood samples.
  • Appropriate use of anticoagulants, like heparin and EDTA.

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Test your knowledge on hematology laboratory safety practices and national patient safety goals. This quiz covers essential procedures to protect both patients and laboratory personnel while ensuring compliance with regulations. Prepare to identify standards for handling bloodborne pathogens effectively.

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