Podcast
Questions and Answers
Staph. Aureus' Gram Stain is Gram _
Staph. Aureus' Gram Stain is Gram _
Staph. Epidermis' Gram Stain is Gram _
Staph. Epidermis' Gram Stain is Gram _
Micro. Luteus's Gram Stain is Gram _
Micro. Luteus's Gram Stain is Gram _
Strep. Pyogenes's Gram Stain is Gram _
Strep. Pyogenes's Gram Stain is Gram _
Strep. Agalactia's Gram Stain is Gram _
Strep. Agalactia's Gram Stain is Gram _
E. Faecalis's Gram Stain is Gram _
E. Faecalis's Gram Stain is Gram _
Strep. Bovis's Gram Stain is Gram _
Strep. Bovis's Gram Stain is Gram _
N. Sicca's Gram Stain is Gram _
N. Sicca's Gram Stain is Gram _
N. Lactamica's Gram Stain is Gram _
N. Lactamica's Gram Stain is Gram _
M. Catarrhalis's Gram Stain is Gram _
M. Catarrhalis's Gram Stain is Gram _
Staph. Aureus on Blood Agar is ____
Staph. Aureus on Blood Agar is ____
Staph. Epidermis on Blood Agar is ____
Staph. Epidermis on Blood Agar is ____
Micro. Luteus on Blood Agar is ____
Micro. Luteus on Blood Agar is ____
Strep. Pyogenes on Blood Agar is ____
Strep. Pyogenes on Blood Agar is ____
Strep. agalactiae on Blood Agar is ____
Strep. agalactiae on Blood Agar is ____
E. faecalis on Blood Agar is ____
E. faecalis on Blood Agar is ____
Strep. Bovis on Blood Agar is ____
Strep. Bovis on Blood Agar is ____
N. sicca on Blood Agar is ____
N. sicca on Blood Agar is ____
N. lactamica on Blood Agar is ____
N. lactamica on Blood Agar is ____
M. catarrhalis on Blood Agar is ____
M. catarrhalis on Blood Agar is ____
Staph. Aureus on Phenyl Ethyl Alcohol (PEA) is ____
Staph. Aureus on Phenyl Ethyl Alcohol (PEA) is ____
Staph. Epidermis on Phenyl Ethyl Alcohol (PEA) is ____
Staph. Epidermis on Phenyl Ethyl Alcohol (PEA) is ____
Micro. Luteus on Phenyl Ethyl Alcohol (PEA) is ____
Micro. Luteus on Phenyl Ethyl Alcohol (PEA) is ____
Strep. Pyogenes on Phenyl Ethyl Alcohol (PEA) is ____
Strep. Pyogenes on Phenyl Ethyl Alcohol (PEA) is ____
Strep. agalactiae on Phenyl Ethyl Alcohol (PEA) is ____
Strep. agalactiae on Phenyl Ethyl Alcohol (PEA) is ____
E. faecalis on Phenyl Ethyl Alcohol (PEA) is ____
E. faecalis on Phenyl Ethyl Alcohol (PEA) is ____
Strep. Bovis on Phenyl Ethyl Alcohol (PEA) is ____
Strep. Bovis on Phenyl Ethyl Alcohol (PEA) is ____
N. sicca on Phenyl Ethyl Alcohol (PEA) is ____
N. sicca on Phenyl Ethyl Alcohol (PEA) is ____
N. lactamica on Phenyl Ethyl Alcohol (PEA) is ____
N. lactamica on Phenyl Ethyl Alcohol (PEA) is ____
M. catarrhalis on Phenyl Ethyl Alcohol (PEA) is ____
M. catarrhalis on Phenyl Ethyl Alcohol (PEA) is ____
Staph. Aureus on Mannitol Salt Agar (MSA) is ____
Staph. Aureus on Mannitol Salt Agar (MSA) is ____
Staph. Epidermis on Mannitol Salt Agar (MSA) is ____
Staph. Epidermis on Mannitol Salt Agar (MSA) is ____
Micro. Luteus on Mannitol Salt Agar (MSA) is ____
Micro. Luteus on Mannitol Salt Agar (MSA) is ____
Strep. Pyogenes on Mannitol Salt Agar (MSA) is ____
Strep. Pyogenes on Mannitol Salt Agar (MSA) is ____
Strep. agalactiae on Mannitol Salt Agar (MSA) is ____
Strep. agalactiae on Mannitol Salt Agar (MSA) is ____
E. faecalis on Mannitol Salt Agar (MSA) is ____
E. faecalis on Mannitol Salt Agar (MSA) is ____
Strep. Bovis on Mannitol Salt Agar (MSA) is ____
Strep. Bovis on Mannitol Salt Agar (MSA) is ____
N. sicca on Mannitol Salt Agar (MSA) is ____
N. sicca on Mannitol Salt Agar (MSA) is ____
N. lactamica on Mannitol Salt Agar (MSA) is ____
N. lactamica on Mannitol Salt Agar (MSA) is ____
M. catarrhalis on Mannitol Salt Agar (MSA) is ____
M. catarrhalis on Mannitol Salt Agar (MSA) is ____
Staph. Aureus' Catalase result is ____
Staph. Aureus' Catalase result is ____
Staph. Epidermis' Catalase result is ____
Staph. Epidermis' Catalase result is ____
Micro. Luteus's Catalase result is ____
Micro. Luteus's Catalase result is ____
Strep. Pyogenes's Catalase result is ____
Strep. Pyogenes's Catalase result is ____
Strep. agalactiae's Catalase result is ____
Strep. agalactiae's Catalase result is ____
E. faecalis's Catalase result is ____
E. faecalis's Catalase result is ____
Strep. Bovis's Catalase result is ____
Strep. Bovis's Catalase result is ____
Staph. Sapro's Coagulase result is ____
Staph. Sapro's Coagulase result is ____
Staph. Aureus' Novobicin result is ____
Staph. Aureus' Novobicin result is ____
Staph. Epidermis' Novobicin result is ____
Staph. Epidermis' Novobicin result is ____
Micro. Luteus's Novobicin result is ____
Micro. Luteus's Novobicin result is ____
Staph. Aureus' Bacitracin result is ____
Staph. Aureus' Bacitracin result is ____
Staph. Epidermis' Bacitracin result is ____
Staph. Epidermis' Bacitracin result is ____
Micro. Luteus's Bacitracin result is ____
Micro. Luteus's Bacitracin result is ____
Strep. pyogenes's Bacitracin result is ____
Strep. pyogenes's Bacitracin result is ____
Strep. Agalactia's Bacitracin result is ____
Strep. Agalactia's Bacitracin result is ____
E. faecalis's Bacitracin result is ____
E. faecalis's Bacitracin result is ____
Strep. bovis's Bacitracin result is ____
Strep. bovis's Bacitracin result is ____
Staph. Sapro's Nitrate result is ____
Staph. Sapro's Nitrate result is ____
Strep. Agalactia's Nitrate result is ____
Strep. Agalactia's Nitrate result is ____
Ν. sicca's Nitrate result is ____
Ν. sicca's Nitrate result is ____
Strep. pyogenes's Bile Esculin result is ____
Strep. pyogenes's Bile Esculin result is ____
Strep. agalactiae's Bile Esculin result is ____
Strep. agalactiae's Bile Esculin result is ____
E. faecalis's Bile Esculin result is ____
E. faecalis's Bile Esculin result is ____
Strep. bovis's Bile Esculin result is ____
Strep. bovis's Bile Esculin result is ____
Strep. pyogenes's Pyrrolidinyl arylamidase (PYR) result is ____
Strep. pyogenes's Pyrrolidinyl arylamidase (PYR) result is ____
E. faecalis's Pyrrolidinyl arylamidase (PYR) result is ____
E. faecalis's Pyrrolidinyl arylamidase (PYR) result is ____
Strep. bovis's Pyrrolidinyl arylamidase (PYR) result is ____
Strep. bovis's Pyrrolidinyl arylamidase (PYR) result is ____
Strep. pyogenes's Hippurate result is ____
Strep. pyogenes's Hippurate result is ____
E. Faecalis's Hippurate result is ____
E. Faecalis's Hippurate result is ____
N. sicca on Modified Thayer-Martin (MTM) exhibits ____
N. sicca on Modified Thayer-Martin (MTM) exhibits ____
N. lactamica on Modified Thayer-Martin (MTM) exhibits____
N. lactamica on Modified Thayer-Martin (MTM) exhibits____
M. catarrhalis on Modified Thayer-Martin (MTM) exhibits____
M. catarrhalis on Modified Thayer-Martin (MTM) exhibits____
N. sicca on Chocolate Agar (CA) exhibits ____
N. sicca on Chocolate Agar (CA) exhibits ____
N. lactamica on Chocolate Agar (CA) exhibits ____
N. lactamica on Chocolate Agar (CA) exhibits ____
M. catarrhalis on Chocolate Agar (CA) exhibits ____
M. catarrhalis on Chocolate Agar (CA) exhibits ____
N. sicca Oxidase result is ____
N. sicca Oxidase result is ____
N. lactamica Oxidase result is ____
N. lactamica Oxidase result is ____
M. catarrhalis Oxidase result is ____
M. catarrhalis Oxidase result is ____
N. sicca's Cysteine Trypticase Agar (CTA) Glucose result is ____
N. sicca's Cysteine Trypticase Agar (CTA) Glucose result is ____
N. lactamica's Cysteine Trypticase Agar (CTA) Glucose result is ____
N. lactamica's Cysteine Trypticase Agar (CTA) Glucose result is ____
M. catarrhalis's Cysteine Trypticase Agar (CTA) Glucose result is ____
M. catarrhalis's Cysteine Trypticase Agar (CTA) Glucose result is ____
Flashcards
What is Gram Stain?
What is Gram Stain?
Determines if bacteria have peptidoglycan, Gram + is thick, Gram - is thin
What is Blood Agar?
What is Blood Agar?
Blood Agar tests for hemolysis. Alpha: partial, Beta: complete, Gamma: none
What is Mannitol Salt Agar?
What is Mannitol Salt Agar?
Used to differentiate organisms based on mannitol fermentation
What does Catalase test for?
What does Catalase test for?
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What does Coagulase test for?
What does Coagulase test for?
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What is a Nitrate test?
What is a Nitrate test?
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What is Bile Esculin?
What is Bile Esculin?
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What is PYR test?
What is PYR test?
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What is Oxidase test?
What is Oxidase test?
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What is MacConkey Agar?
What is MacConkey Agar?
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Study Notes
Gram Staining and Growth Characteristics
- Staph. aureus is Gram-positive and exhibits beta-hemolysis on blood agar.
- Staph. epidermidis is Gram-positive and shows gamma-hemolysis on blood agar.
- Staph. saprophyticus is Gram-positive and presents gamma-hemolysis on blood agar.
- Micro. luteus is Gram-positive.
- Strep. pyogenes is Gram-positive and displays beta-hemolysis on blood agar.
- Strep. agalactiae is Gram-positive and has beta-hemolysis on blood agar.
- E. faecalis is Gram-positive and exhibits gamma-hemolysis on blood agar.
- Strep. bovis is Gram-positive and shows gamma-hemolysis on blood agar.
- N. sicca is Gram-positive.
- N. lactamica is Gram-negative.
- M. catarrhalis is Gram-negative.
Physiological and Biochemical Tests
- Staph. aureus, Micro. luteus, and Staph. saprophyticus produce yellow colonies on Mannitol Salt Agar, indicating mannitol fermentation. Staph. epidermidis produces pink colonies.
- Strep. pyogenes, Strep. agalactiae, and E. faecalis grow on Mannitol Salt Agar but don't ferment mannitol. Strep. bovis does not grow on Mannitol Salt Agar.
- Staph. aureus is catalase-positive (bubbles). Staph. epidermidis and Staph. saprophyticus also produce bubbles with catalase.
- Staph. aureus produces Clots while Staph. epidermidis and Staph. saprophyticus do not.
- Staph. aureus is novobiocin-sensitive, Staph. epidermidis is novobiocin-sensitive, and Staph. saprophyticus is novobiocin-resistant.
- Staph. aureus is bacitracin-resistant and Nitrate positive.
- Strep. pyogenes and Strep. agalactiae are CAMP negative, bile esculin negative, and PYR negative. Strep. agalactiae gives a positive Hippurate test. Strep. pyogenes gives a negative Hippurate test.
- E. faecalis shows growth and blackening on bile esculin agar and is PYR positive (red).
- Strep. bovis exhibits growth and blackening on bile esculin agar and is PYR negative.
- N. sicca, N. lactamica, and M. catarrhalis grow on Modified Thayer-Martin (MTM) and Chocolate Agar (CA).
- N. sicca, N. lactamica, and M. catarrhalis are oxidase-positive.
Additional Biochemical Tests
- C. freundii is oxidase-negative, K/A with H2S on TSI.
- E. coli is oxidase-negative, A/A with gas on TSI.
- E. aerogenes is oxidase-negative, produces A/A and H2S with gas on TSI.
- Proteus vulgaris is oxidase-negative, produces A/A and H2S with gas on TSI.
- Providencia rettgari is oxidase-negative, K/A on TSI, is Indole and Citrate positive.
- Salmonella typhimurium is oxidase-negative, K/A with H2S on TSI.
- Shigella sonnei is oxidase-negative, K/A on TSI, is indole Negative
- Proteus mirabilis is oxidase-negative, K/A H2S, Citrate and Urease positive.
- Serratia marcescens is oxidase-negative, has A/A/Yellow (Yellow/Yellow) on TSI is Dnase -positive.
- E. coli is Indole and SIM-positive, Voges-Proskauer and Citrate-Negative
- E. aerogenes is Voges-Proskauer and Citrate -positive, indole negative.
- Proteus vulgaris is SIM-positive (H2S production).
- Serratia produces Lysine and is Positive with Growth on MacConkey agar.
- E. Coli does not produce Lysine and gives a Positive on MacConkey agar.
- E. Aerogenes produces Lysine and gives a Positive on MacConkey agar.
- Proteus vulgaris produces Lysine and gives a Positive on MacConkey agar.
- C. Freundii is negative for Lysine and MacConkey is Pink Growth
- Salmonella Typhimurium is negative for Lysine and also MacConkey is negative (clear growth)
- Shigella doesn't produce Lysine and grows negative on MacConkey agar.
- Proteus mirabilis produces Lysine and produces a growth on MacConkey.
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