Gram-Positive Bacteria: Bacteriology Quiz
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Questions and Answers

What is the reason behind gram-positive bacteria appearing purple after the Gram stain test?

  • They retain the crystal violet stain due to their thick peptidoglycan layer (correct)
  • They have a thinner peptidoglycan layer that retains the crystal violet stain
  • They have an outer membrane that retains the crystal violet stain
  • They take up the counterstain (safranin or fuchsine) and appear red or pink

    • Why do gram-negative bacteria appear red or pink after the Gram stain test?

  • They have an outer membrane that retains the crystal violet stain
  • They retain the counterstain (safranin or fuchsine) and appear red or pink (correct)
  • They cannot retain the crystal violet stain due to their thin peptidoglycan layer
  • They have a thicker peptidoglycan layer that retains the crystal violet stain

    • What effect does alcohol have on gram-negative bacteria during the decolorization stage of the Gram stain test?

  • It degrades the outer membrane, making the cell wall more porous (correct)
  • It causes them to retain the crystal violet stain
  • It thickens their peptidoglycan layer
  • It makes them appear red or pink

    • What is the main difference in cell wall structure between gram-positive and gram-negative bacteria?

    <p>Thickness of peptidoglycan layer and presence of an outer membrane</p> Signup and view all the answers

    Why are gram-positive bacteria more receptive to certain cell wall–targeting antibiotics than gram-negative bacteria?

    <p>Due to the absence of an outer membrane</p> Signup and view all the answers

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