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Glutamic Acid Production Bio-Synthesis Process Quiz
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Glutamic Acid Production Bio-Synthesis Process Quiz

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Questions and Answers

What are the two species commonly used for glutamic acid production in bio synthesis?

Corynebacterium glutamicum and Brevibacterium divericartum

Why is fed batch fermentation preferred over batch fermentation for amino acid production?

Fed batch fermentation is preferred because most amino acid production requires high sugar concentration, and adding all sugar at once can inhibit growth of microorganisms and reduce yield.

What is the role of biotin in glutamic acid production?

Production of glutamic acid is greatest when biotin is limited, i.e., suboptimal.

What is the significance of the lag phase in glutamic acid production?

<p>During the lag phase, oxygen demand increases but growth is reduced.</p> Signup and view all the answers

Why is the temperature cooled to 30 degrees Celsius before adding the microorganism in glutamic acid production?

<p>Colder temperatures provide an optimal environment for the microorganism to grow and produce glutamic acid.</p> Signup and view all the answers

What is the purpose of isocitrate-succinate in the TCA cycle?

<p>Needed for growth</p> Signup and view all the answers

How can permeability be enhanced in cells for downstream processing?

<p>By ensuring biotin deficiency in the medium, treating with fatty acid derivatives, mutants with oleic acid deficiency, and addition of penicillin during growth phase.</p> Signup and view all the answers

What is the method used for recovery of amino acids after fermentation?

<p>Crystallization</p> Signup and view all the answers

How are ion exchange resins used in the purification and extraction of amino acids?

<p>Adsorption influenced by pH and presence of contaminant ions; cation exchange resins bind to positively charged amino acids, while anion exchange resins bind to negatively charged amino acids.</p> Signup and view all the answers

What is the significance of poly glutamic acid?

<p>Naturally occurring anionic polymer, biodegradable, non-toxic towards the environment.</p> Signup and view all the answers

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