Untitled Quiz

<p>They are specifically suited to particular research goals. (C)</p> Signup and view all the answers

<p>They could lead to unreliable data and conclusions. (D)</p> Signup and view all the answers

<p>Greater ability to function under varied conditions. (D)</p> Signup and view all the answers

<p>It can facilitate the creation of novel test methods. (C)</p> Signup and view all the answers

<p>The potential for high cross-dimerization. (C)</p> Signup and view all the answers

<p>The assay is 100% efficient (B)</p> Signup and view all the answers

<p>Four to five replicates (A)</p> Signup and view all the answers

<p>It shows how efficient the PCR reaction is (D)</p> Signup and view all the answers

<p>Avoiding any primer dimers (D)</p> Signup and view all the answers

<p>It will result in higher Cq values (B)</p> Signup and view all the answers

<p>A gradient recording similar q values (B)</p> Signup and view all the answers

<p>It decreases the standard curve accuracy (B)</p> Signup and view all the answers

<p>Good primers design (C)</p> Signup and view all the answers

<p>To differentiate between amplicon and primer dimer. (C)</p> Signup and view all the answers

<p>95% - 105% (B)</p> Signup and view all the answers

<p>A linear relationship between initial template quantity and Cq value. (C)</p> Signup and view all the answers

<p>Consistency across replicate experiments. (D)</p> Signup and view all the answers

<p>By a linear relationship between Cq value and template quantity. (D)</p> Signup and view all the answers

<p>Cq value is directly tied to a specific copy number. (C)</p> Signup and view all the answers

<p>Using serial dilutions of a template. (B)</p> Signup and view all the answers

<p>It ensures reliability of results across different labs. (D)</p> Signup and view all the answers

A statistical measure that quantifies the proportion of variance in a dependent variable that's explained by an independent variable in a regression model.

Real-time PCR, a technique used to quantify the amount of DNA or RNA in a sample by measuring the fluorescence signal during amplification.

Next-generation sequencing, a group of high-throughput DNA sequencing technologies that enable the sequencing of large numbers of DNA fragments simultaneously.

Short, single-stranded DNA molecules used to initiate PCR amplification of a specific DNA sequence.

A specially designed assay tailored to specific research objectives, often more effective than commercially available assays in the long run.

The process of confirming the accuracy, precision, and reliability of an assay, usually including validation reports.

qPCR's ability to detect low copy number targets.

The length of the DNA segment amplified during qPCR.

Formation of unintended primer pairs that compete with the target for amplification.

Measure of how well the qPCR reaction amplifies the target.

A graph showing the relationship between the log of the initial template quantity and the Cq value.

The range of template quantities that can be accurately measured using qPCR.

Amplification efficiency of 95-105% considered good for qPCR.

A standard curve with an R-squared value greater than 0.980, showcasing a reliable relationship in qPCR.

Reproducibility of results across multiple independent experiments.

A method of reducing the concentration of a substance by a factor of ten.

A measure of how effectively a polymerase chain reaction (PCR) amplifies a target DNA sequence.

A graph showing a linear relationship between the measured quantity and known concentrations of DNA.

A measure of how well the data fits a linear model.

The cycle number at which a fluorescent signal from the PCR reaction crosses a threshold.

A byproduct of PCR that forms when primers bind to each other.

The process of planning and creating a set of experiments to quantify nucleic acids and measure PCR efficiency.

A method to evaluate PCR reaction specificity.

The way genes are organized into coding regions (exons) and non-coding regions (introns).