Podcast
Questions and Answers
What is the term for the physical compaction of genomes where each chromosome has its own 'space or neighbourhood' within the nucleus?
What is the term for the physical compaction of genomes where each chromosome has its own 'space or neighbourhood' within the nucleus?
- Chromosomal segregation
- Spatial chromatin organization (correct)
- Nuclear zoning
- Genomic condensation
Which area of a chromosome is generally genetically active?
Which area of a chromosome is generally genetically active?
- Nuclear periphery chromatin
- Euchromatin (correct)
- Nucleolus-associated chromatin
- Heterochromatin
What do interphase chromosomes occupy within the nucleus?
What do interphase chromosomes occupy within the nucleus?
- Random positions
- Interconnected webs
- Discrete territories (correct)
- Concentric rings
What is the term for the evolutionarily controlled process of packaging DNA into a particular space within the nucleus?
What is the term for the evolutionarily controlled process of packaging DNA into a particular space within the nucleus?
Which protein associates with the Stag protein of cohesin?
Which protein associates with the Stag protein of cohesin?
What is the chromatin residence time of cohesin?
What is the chromatin residence time of cohesin?
What results from the loss of insulation between TADs after cohesin and CTCF knock-down?
What results from the loss of insulation between TADs after cohesin and CTCF knock-down?
What model supports the theory of 'stable' TAD structures?
What model supports the theory of 'stable' TAD structures?
What forms between convergent CTCF sites, thus regulating structure specificity?
What forms between convergent CTCF sites, thus regulating structure specificity?
Which factor does NOT localise at CTCF sites, suggesting cohesin must travel on chromatin to reach CTCF?
Which factor does NOT localise at CTCF sites, suggesting cohesin must travel on chromatin to reach CTCF?
What is associated with transient loops within TADs (intra-TAD DNA loops) and influences specific gene expression?
What is associated with transient loops within TADs (intra-TAD DNA loops) and influences specific gene expression?
Which technique highlights the co-localisation of cohesin and CTCF at TAD boundaries?
Which technique highlights the co-localisation of cohesin and CTCF at TAD boundaries?
What can Cohesin associate with to influence specific gene expression?
What can Cohesin associate with to influence specific gene expression?
What do Population-based Chromatin Conformation Capture techniques create?
What do Population-based Chromatin Conformation Capture techniques create?
What is the function of cohesin during the G1 phase?
What is the function of cohesin during the G1 phase?
What can methylation of a CTCF site dictate?
What can methylation of a CTCF site dictate?
What is the role of topologically associated domains (TADs)?
What is the role of topologically associated domains (TADs)?
What do enhancers do in the context of long-range gene regulation?
What do enhancers do in the context of long-range gene regulation?
What is the biological importance of TADs/structure?
What is the biological importance of TADs/structure?
What is the function of CTCF during the G1 phase?
What is the function of CTCF during the G1 phase?
What does cohesin do during DNA replication?
What does cohesin do during DNA replication?
'CTCF Code': x11 Zinc Finger protein gives multivalence to CTCF's recognition sequence, which fine-tunes its insulator 'strength'. What does this statement imply?
'CTCF Code': x11 Zinc Finger protein gives multivalence to CTCF's recognition sequence, which fine-tunes its insulator 'strength'. What does this statement imply?
What are the functions of TADs?
What are the functions of TADs?
What is the role of enhancers in long-range gene regulation?
What is the role of enhancers in long-range gene regulation?
What dictates the ability of cohesin to interact with DNA in trans and cis?
What dictates the ability of cohesin to interact with DNA in trans and cis?
What does methylation of a CTCF site protect from?
What does methylation of a CTCF site protect from?
