Podcast
Questions and Answers
What was the first FDA approved genetically modified food?
What was the first FDA approved genetically modified food?
The Flavr Savr Tomato was introduced to produce a higher level of polygalacturonase.
The Flavr Savr Tomato was introduced to produce a higher level of polygalacturonase.
False
Who began the Golden Rice Project?
Who began the Golden Rice Project?
Ingo Potrykus and Peter Beyer
Golden Rice is produced through genetic engineering to biosynthesize ____ in the edible parts of rice.
Golden Rice is produced through genetic engineering to biosynthesize ____ in the edible parts of rice.
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What is the main goal of biofortification?
What is the main goal of biofortification?
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What are the symptoms of vitamin A deficiency?
What are the symptoms of vitamin A deficiency?
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Rice is considered a global staple food cultivated for over 1,000 years.
Rice is considered a global staple food cultivated for over 1,000 years.
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Nearly ____ million children die from malnutrition each year.
Nearly ____ million children die from malnutrition each year.
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Which organizations funded the Golden Rice Project?
Which organizations funded the Golden Rice Project?
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Which gene from daffodil was used in the production of Golden Rice?
Which gene from daffodil was used in the production of Golden Rice?
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The lack of required enzymes prevents rice embryos from synthesizing ____ or beta-carotene.
The lack of required enzymes prevents rice embryos from synthesizing ____ or beta-carotene.
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Study Notes
Flavr Savr Tomato
- First genetically modified food approved by the FDA in 1994.
- Designed to delay the softening and rotting process through the inhibition of the enzyme polygalacturonase (PG).
- Calgene introduced an antisense RNA (asRNA) which is complementary to the PG mRNA, reducing PG enzyme synthesis.
- The transformation involved in vitro transcription, RNA isolation, hybridization, and measurement of PG activity.
Constructs and Components
- Utilized constructs such as pCGN1436, pCGN1547, pCGN1548, pCGN1549, pCGN1557, pCGN1558, pCGN1559, pCGN1578, and pCGN4109 for genetic modification.
- Key components of pCGN4109 include:
- LB (left border)
- RB (right border)
- nptII (neomycin phosphotransferase)
- pg (polygalacturonase)
- 35S CaMV (promoter)
- T-Tml (termination sequences from plasmid pTiA6).
Effects of Malnutrition
- Vitamin A deficiency (VAD) leads to symptoms such as night blindness, increased cancer risk, anemia, tissue deterioration, and cardiovascular issues.
- Nearly 9 million children die from malnutrition annually, often due to preventable diseases resulting from inadequate nutrition.
- Reduced immune competence significantly raises morbidity and mortality rates in children.
Golden Rice Project
- Initiated in 1982 by Ingo Potrykus and Peter Beyer, funded by several organizations including the Rockefeller Foundation.
- Aims to introduce and distribute biofortified rice enriched with vitamin A in regions with dietary shortages.
Importance of Rice
- Rice is a global staple, cultivated for over 10,000 years, serving as a primary calorie source for over 3 billion people.
- Other plants like sweet potatoes showcase varying pro-vitamin A content, underlining rice's importance in dietary nutrition.
Golden Rice Characteristics
- Genetically engineered to synthesize beta-carotene (provitamin A) in the edible part (endosperm) of rice.
- The orange color of golden rice results from the accumulation of beta-carotene.
- Developed at ETH Zurich, utilizing genes from daffodil and other sources to facilitate beta-carotene biosynthesis.
Genetic Engineering of Golden Rice
- Golden rice production involved transforming rice with phytoene synthase (psy) from daffodil and a second gene for beta-carotene synthesis.
- The rice plant is capable of producing beta-carotene in leaves but requires modification for production in the endosperm.
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Description
Explore the significance of genetically modified foods with a focus on key examples like the Flavr Savr tomato, Golden Rice, and Bt Cotton. This quiz delves into their development, FDA approval, and the genetic modifications that enhance their traits. Test your knowledge on the science behind GMOs and their impact on agriculture.
