Genetically Engineered Products Overview
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Questions and Answers

Who established the model of two helically intertwined chains tied together by hydrogen bonds between the purines and pyrimidines in DNA?

  • Watson and Crick (correct)
  • Rosalind Franklin and Maurice Wilkins
  • William Astbury
  • Hershey and Chase
  • What was the key contribution of the 'blender experiment' by Hershey and Chase?

  • Identifying the role of reverse transcriptase in viral replication.
  • Demonstrating that DNA, not protein, carries genetic information. (correct)
  • Discovering restriction enzymes in bacteria.
  • Developing the first recombinant DNA technology.
  • What is the significance of Werner Arber's discovery of restriction enzymes?

  • It paved the way for understanding X-ray diffraction patterns.
  • It led to the development of the autoclave.
  • It enabled the manipulation and splicing of genetic material. (correct)
  • It established the basic principles of inheritance.
  • What year saw the birth of the first biotechnology company, Genentech?

    <p>1976 (D)</p> Signup and view all the answers

    In what year did Paul Berg successfully demonstrate the splicing and recombination of genetic material?

    <p>1971 (D)</p> Signup and view all the answers

    What was the main contribution of the Koch lab in the early development of biotechnology?

    <p>Use of agar as a medium. (A)</p> Signup and view all the answers

    What was the role of X-ray crystallography in the development of genetic engineering?

    <p>It helped determine the structure of DNA. (C)</p> Signup and view all the answers

    Who coined the term 'molecular biology'?

    <p>William Astbury (B)</p> Signup and view all the answers

    What is the name of the first transgenic dairy cow created by GenPharm International?

    <p>None of the above (D)</p> Signup and view all the answers

    What is the name of the first mammalian clone born in 1997?

    <p>Dolly (A)</p> Signup and view all the answers

    What is the name of the gene therapy that was first authorized in the 1990s?

    <p>ADA deficiency therapy (A)</p> Signup and view all the answers

    In what year did Eli Lilly’s recombinant human insulin appear on the market?

    <p>1982 (C)</p> Signup and view all the answers

    In what year was the Human Genome Project launched?

    <p>1986 (D)</p> Signup and view all the answers

    What is the term coined in 1919 by Karl Ereky to describe the manipulation of genetic material?

    <p>Genetic Engineering (A)</p> Signup and view all the answers

    What is the primary purpose of combining DNA from different species in recombinant DNA technology?

    <p>Producing new genetic combinations (B)</p> Signup and view all the answers

    Which of the following is NOT a field where recombinant DNA technology has applications?

    <p>Astronomy (D)</p> Signup and view all the answers

    Which of the following practices represents the earliest form of genetic engineering, predating modern techniques?

    <p>Artificial selection of animals like horses and camels (A)</p> Signup and view all the answers

    What is the role of bacteria or yeast cells in recombinant DNA technology?

    <p>They act as carriers to propagate the recombinant DNA. (A)</p> Signup and view all the answers

    What is the primary contribution of the ancient Hindu philosophers towards the understanding of genetics?

    <p>They established the foundations of genetics by observing hereditary patterns of diseases. (B)</p> Signup and view all the answers

    Based on the text, what key development in the 19th century significantly shifted the focus of biological studies?

    <p>The rise of the fermentation industry. (A)</p> Signup and view all the answers

    What specific technique is used in the creation of glow-in-the-dark cats?

    <p>Viral vector insertion (B)</p> Signup and view all the answers

    Which of the following is NOT a core objective of genetic engineering?

    <p>To create organisms that can only reproduce asexually. (B)</p> Signup and view all the answers

    What is the key characteristic of a seedless grape that makes it desirable?

    <p>Increased sweetness (B)</p> Signup and view all the answers

    What is the main purpose of genetic engineering?

    <p>To improve the characteristics of organisms for human benefit (C)</p> Signup and view all the answers

    What is the primary function of plasmids in bacteria?

    <p>To replicate independently and carry genes for antibiotic resistance. (D)</p> Signup and view all the answers

    What was the significance of the experiments conducted by Stanley Cohen and Herbert Boyer in the 1970s?

    <p>They successfully created the first genetically modified organisms using recombinant DNA technology. (B)</p> Signup and view all the answers

    What is the primary role of restriction endonucleases in recombinant DNA technology?

    <p>To identify and cut specific DNA sequences. (C)</p> Signup and view all the answers

    Why was the transfer of a frog gene into bacteria a significant milestone in recombinant DNA technology?

    <p>It demonstrated that genetic material can be transferred between unrelated organisms. (C)</p> Signup and view all the answers

    What is the primary benefit of using recombinant antibodies in the context of food resources?

    <p>They can increase crop yield and resistance to pests and diseases. (C)</p> Signup and view all the answers

    What is the key difference between chromosomal DNA and plasmids?

    <p>Chromosomal DNA carries essential genes for bacterial survival, while plasmids carry non-essential genes. (A)</p> Signup and view all the answers

    How did the discovery of plasmids and restriction endonucleases contribute to the development of recombinant DNA technology?

    <p>They provided the necessary tools for isolating, manipulating, and inserting genes into bacteria. (D)</p> Signup and view all the answers

    What is the primary process involved in the creation of recombinant DNA molecules?

    <p>The combination of DNA fragments from different sources. (D)</p> Signup and view all the answers

    Which method of gene transfer is particularly advantageous for introducing DNA into large cells, such as eggs or embryos?

    <p>Microinjection (A)</p> Signup and view all the answers

    What is the primary application of protoplast fusion in agriculture?

    <p>Generating hybrid plants with desirable traits (A)</p> Signup and view all the answers

    Which of the following is NOT a direct application of recombinant DNA technology in the health and medicine sector?

    <p>Genetic manipulation of crops for pest resistance (A)</p> Signup and view all the answers

    What is the primary function of bacteriophages in the process of transduction?

    <p>To deliver genetically engineered DNA into bacterial cells (A)</p> Signup and view all the answers

    Which of the following methods is primarily used for introducing genes into plant cells?

    <p>Protoplast fusion (B)</p> Signup and view all the answers

    What is the key difference between electroporation and protoplast fusion?

    <p>Electroporation uses electrical pulses, while protoplast fusion uses chemical agents. (B)</p> Signup and view all the answers

    What is the primary objective of pharmacogenomics in the context of recombinant DNA technology?

    <p>To understand how genes influence a person's response to drugs (A)</p> Signup and view all the answers

    Which of the following best describes the process of gene therapy?

    <p>Replacing a defective gene with a normal functional gene (B)</p> Signup and view all the answers

    What is the role of restriction enzymes in recombinant DNA technology?

    <p>They cut DNA at specific sites, creating fragments. (C)</p> Signup and view all the answers

    What is the purpose of using plasmids in recombinant DNA technology?

    <p>To produce proteins in large quantities. (D)</p> Signup and view all the answers

    What is the role of DNA ligase in recombinant DNA technology?

    <p>To join DNA fragments together. (A)</p> Signup and view all the answers

    What is the primary advantage of using PCR in recombinant DNA technology?

    <p>It allows scientists to amplify DNA sequences, producing millions of copies. (A)</p> Signup and view all the answers

    Which of the following techniques describes the insertion of recombinant DNA into a host cell?

    <p>Transformation (D)</p> Signup and view all the answers

    Electroporation is a technique used for:

    <p>Introducing genetic material into cells. (B)</p> Signup and view all the answers

    Which of the following is NOT a step in the process of recombinant DNA technology?

    <p>Replication of host cells. (C)</p> Signup and view all the answers

    What is the difference between blunt-ended fragments and cohesive fragments?

    <p>Blunt-ended fragments have no overhanging nucleotides, and cohesive fragments have overhanging nucleotides. (B)</p> Signup and view all the answers

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    Flashcards

    Genetic Engineering

    Manipulating an organism's DNA to achieve desired traits.

    Recombinant DNA

    DNA that has been artificially made by combining DNA from different sources.

    Glow-in-the-dark cats

    Cats engineered to produce fluorescent protein from jellyfish DNA.

    Applications of Genetic Engineering

    Uses include medicine, agriculture, and environmental management.

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    Pros of Genetic Engineering

    Advantages include disease resistance, higher yields, and enhanced traits.

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    Cons of Genetic Engineering

    Potential risks include ethical concerns and ecological impacts.

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    Yeast in fermentation

    Yeast has been used since 6000 B.C. for producing beer.

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    Artificial Selection

    The process of breeding organisms for desired traits.

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    Plasmids

    Extra loops of DNA in bacteria that replicate independently.

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    Restriction endonucleases

    Enzymes that cut DNA at specific sequences.

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    Cohen-Boyer experiments

    Pioneering studies that created the first recombinant DNA in 1973.

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    Molecular chimeras

    Organisms created by transferring genes between different species.

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    Biotechnology industry birth

    Emergence of biotech due to recombinant DNA advancements.

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    Gene insertion technique

    Process of inserting genes into plasmids to create recombinant DNA.

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    Antibiotic resistance genes

    Genes that provide bacteria the ability to resist antibiotics.

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    Polymerase Chain Reaction (PCR)

    A technique invented in 1983 by Karen Mullis to amplify DNA segments.

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    Recombinant DNA Technology

    The joining of DNA from different organisms to create new genetic combinations.

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    Transgenic Organisms

    Organisms that have been genetically modified to contain genes from other species.

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    Human Genome Project

    An international scientific research project to map all genes of the human genome.

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    Gene Therapy

    An experimental technique to treat or prevent disease by altering genes.

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    Dolly the Sheep

    The first mammal to be cloned from a somatic cell, born in 1997.

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    Recombinant Interferon Gamma

    A therapeutic protein produced using recombinant DNA technology, used in treatments.

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    Adenosine Deaminase Deficiency (ADA)

    A genetic disorder treated with the first authorized gene therapy in the 1990s.

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    Vectorless Gene Transfer

    A method that uses physical or chemical means to transfer DNA into cells without vectors.

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    Protoplast Fusion

    The process of fusing the protoplasts of different plant species to create hybrids.

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    Microinjection

    A technique to inject DNA directly into cells using a fine needle.

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    Transduction

    Introducing engineered bacteriophages into cells to create recombinant DNA.

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    Cyanobacteria in Industry

    Modified to produce plastics and fuels as byproducts of photosynthesis.

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    Recombinant Insulin

    Insulin produced through recombinant DNA technology for diabetic patients.

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    Pharmacogenomics

    The study of how genes affect responses to drugs.

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    Restriction Enzymes

    Molecular scissors used to cut DNA at specific sequences for gene manipulation.

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    DNA Ligase

    An enzyme that joins two pieces of DNA together during genetic engineering.

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    Isolation of Genetic Material

    The first step in recombinant DNA technology where desired DNA is purified.

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    Amplifying the Gene

    The process of creating multiple copies of a specific DNA segment using PCR.

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    Ligation

    The step that involves joining cut DNA fragments with a vector using DNA ligase.

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    Transformation

    The process of inserting recombinant DNA into a host cell.

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    Electroporation

    A method to introduce DNA into cells using an electric field for enhanced permeability.

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    Mendel's Pea Experiments

    Mendel presented his genetic findings using pea plants in 1864-1865.

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    Autoclave Development

    An invention in 1884 by Chamberland for sterilization in labs.

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    X-ray Discovery

    W. Roentgen discovered X-rays in 1895, crucial for imaging.

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    Molecular Biology Term

    Coined by William Astbury in 1945, studying macromolecule structures.

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    Hershey-Chase Experiment

    The 1951 experiment showing DNA is hereditary material.

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    Restriction Enzymes Identification

    Werner Arber identified these enzymes in the late 1960s for DNA cleavage.

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    Reverse Transcriptase

    Identified independently by Temin and Baltimore in 1970; crucial for recombinant DNA.

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    First Recombinant DNA

    Produced in 1972 at Boyer Laboratory, marking a milestone in genetics.

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    Study Notes

    Assignment Instructions

    • Create a one-page document.
    • List at least five genetically engineered products in Medicine, Agriculture, Industry or Environment.
    • Choose one product and explain its engineering, benefits and drawbacks.

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    Recombinant DNA PDF

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    Explore various genetically engineered products across medicine, agriculture, industry, and environment. This quiz provides an overview of at least five products and asks you to delve deeper into one, discussing its engineering, benefits, and drawbacks. Test your knowledge and understanding of genetic engineering in various fields.

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