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What types of restriction enzymes were used to digest the liver DNA from mouse 23-2?
What types of restriction enzymes were used to digest the liver DNA from mouse 23-2?
Restriction enzymes that cut pMK twice (Bst EII, Eco RI, Pvu II), once (Bam HI, Bgl II, Kpn I), or not at all (Hind III, Xba I).
What was used as a probe in the hybridization process after electrophoresis?
What was used as a probe in the hybridization process after electrophoresis?
A pMK(-EK) nick-translated probe.
What are the major bands in the Pvu II digests of liver, testis, and muscle DNA from mouse 23-2?
What are the major bands in the Pvu II digests of liver, testis, and muscle DNA from mouse 23-2?
The major bands are restriction fragments predicted from the endogenous MT-I gene and from cleavage within the repeats of pMK.
What is illustrated in Figure 4 of the text?
What is illustrated in Figure 4 of the text?
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How was the major bands inset obtained in the experiment?
How was the major bands inset obtained in the experiment?
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Which of the following is a likely reason for using phage λ DNA digested with Hind III as a size marker?
Which of the following is a likely reason for using phage λ DNA digested with Hind III as a size marker?
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What is the likely purpose of using the MT-I gene plasmid m1pEE3.8 as a size marker?
What is the likely purpose of using the MT-I gene plasmid m1pEE3.8 as a size marker?
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Based on the text, what technique was likely used to determine the mRNA levels of the MT-I gene?
Based on the text, what technique was likely used to determine the mRNA levels of the MT-I gene?
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Which of the following statements is most likely true based on the information provided?
Which of the following statements is most likely true based on the information provided?
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Which of the following techniques was likely used to introduce the Xenopus 5S RNA gene into mouse oocytes?
Which of the following techniques was likely used to introduce the Xenopus 5S RNA gene into mouse oocytes?
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