10 Questions
What types of restriction enzymes were used to digest the liver DNA from mouse 23-2?
Restriction enzymes that cut pMK twice (Bst EII, Eco RI, Pvu II), once (Bam HI, Bgl II, Kpn I), or not at all (Hind III, Xba I).
What was used as a probe in the hybridization process after electrophoresis?
A pMK(-EK) nick-translated probe.
What are the major bands in the Pvu II digests of liver, testis, and muscle DNA from mouse 23-2?
The major bands are restriction fragments predicted from the endogenous MT-I gene and from cleavage within the repeats of pMK.
What is illustrated in Figure 4 of the text?
Models for Integration of pMK DNA into Mouse DNA.
How was the major bands inset obtained in the experiment?
The major bands inset was obtained after a 14 hr exposure compared to 60 hr for the main figure.
Which of the following is a likely reason for using phage λ DNA digested with Hind III as a size marker?
It provides a well-characterized set of fragment sizes for comparison.
What is the likely purpose of using the MT-I gene plasmid m1pEE3.8 as a size marker?
To provide a set of restriction fragments with known sizes for size estimation.
Based on the text, what technique was likely used to determine the mRNA levels of the MT-I gene?
Northern blotting
Which of the following statements is most likely true based on the information provided?
The MT-I gene encodes a protein involved in metal ion homeostasis.
Which of the following techniques was likely used to introduce the Xenopus 5S RNA gene into mouse oocytes?
Microinjection
This quiz covers a genetic experiment to estimate the number of MK genes per cell using densitometry and hybridization. The experiment involves comparing the intensity of MK and MT-I gene bands in mice and conducting control experiments with equal molar amounts of genes.
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