29 Questions
What is the main focus of genetic engineering?
Altering the genetic make-up of organisms
Which agricultural practice has significantly changed the genetic make-up of crops and livestock?
Selective breeding
What is one of the ways genetic engineering can enhance traits in an organism?
Disrupting the inhibition of the desired gene's expression
Which of the following activities is related to identifying desirable traits in plants and animals?
Selective Breeding or Classical Breeding
Why did humans unknowingly practice genetic engineering in ancient times?
To alter genetic make-up through selective breeding
Which technique involves introducing new traits into an organism using molecular methods?
Genetic Engineering
What is the name given to the molecule resulting from the combination of DNA from two different sources?
Recombinant DNA
Which type of cell is used as the host for the recombinant plasmid?
Bacterium
What is the purpose of the selection process after cloning?
To identify which cells contain the desired gene
What is determined by sequencing the gene?
The primary structure of the protein
What is the final step after the recombinant DNA process?
Introducing the recombinant plasmids to host organisms
What is the term used for a bacterium carrying the recombinant plasmid?
Recombinant bacterium
What is the purpose of using restriction enzymes in recombinant DNA technology?
To cut and cleave DNA at specific target sequences
Which of the following is commonly used as a vector in recombinant DNA technology?
Plasmids
What is the process of joining the gene of interest and the vector DNA called?
Ligation
Which of the following is NOT a characteristic of plasmids used as vectors in recombinant DNA technology?
They are part of the main cellular genome
What is the purpose of using a vector in recombinant DNA technology?
To propagate the recombinant DNA within a host organism
Classical breeding and recombinant DNA technology are both techniques used for:
Genetic engineering
What is the purpose of inserting a gene for pest resistance into a plant cell?
To make the plant resistant to pests
What technique is used to introduce DNA into plant tissues using a 'gene gun'?
Biolistic transformation
What is the purpose of the heat shock treatment in bacterial transformation?
To increase the pore sizes of the plasma membrane
Which of the following is a product that can be obtained through bacterial transformation?
Both b and c
What term is used to describe cells that have successfully taken up a plasmid and acquired new traits?
Transformed
Why is it important to control the temperature during the heat shock treatment?
To facilitate the entry of the plasmid DNA into the cells
What is the main difference between Electroporation and Heat Shock Treatment?
Electroporation uses an electric shock to expand membrane pores, while Heat Shock Treatment uses heat.
Which method is commonly used for inserting genes into mammalian cells?
Electroporation
From which book is information on Electroporation likely to be found?
Molecular Biology of the Cell by Alberts et al.
What is the purpose of expanding membrane pores in Electroporation?
To allow the insertion of genes into cells
Which grade guide is referenced for the course criteria in the text?
DepEd Guide on Grading System for Science Projects
Test your knowledge on the history and practice of genetic engineering in agriculture, including selective breeding and altering genetic make-up of organisms. Learn about how ancient humans have been engaging in genetic engineering for centuries.
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