Genetic Diversity and Evolution Quiz
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Questions and Answers

What is the main reason diverse behaviors and different pathways in multicellular organisms might necessitate a more complex genetic toolkit?

  • To facilitate precise positioning of DNA in cells
  • To allow for interactions potentially leading to co-evolution with symbiotic relationships (correct)
  • To break down cells mechanically for DNA extraction purposes
  • To ensure lateral gene transfer between different organisms
  • What is the primary purpose of using enzymes like proteinase K or lysozyme during DNA extraction from eukaryotic cells?

  • To remove soluble components
  • To disrupt the cell membrane (correct)
  • To facilitate lateral gene transfer
  • To isolate supermatant
  • What is the role of sodium acetate in DNA extraction when added to the precipitate?

  • Breaking down DNA mechanically
  • Dissolving the precipitate (correct)
  • Isolating supermatant
  • Detaching DNA from proteins
  • Why are restriction enzymes important in DNA manipulation processes?

    <p>To cut DNA at specific sites</p> Signup and view all the answers

    What method is typically used to separate released debris from soluble components during DNA extraction?

    <p>Centrifugation</p> Signup and view all the answers

    In which cellular compartment is DNA typically located in eukaryotic cells before extraction?

    <p>Nucleus</p> Signup and view all the answers

    What is the main characteristic of a palindrome sequence recognized by restriction enzymes?

    <p>It reads the same in the 5' to 3' direction on one strand and in the 3' to 5' direction on the complementary strand.</p> Signup and view all the answers

    How do DNA fragments with sticky ends interact with each other?

    <p>They can pair with a single-stranded overhang from another DNA piece cut by the same restriction enzyme.</p> Signup and view all the answers

    What is the purpose of methylation in relation to recognition by restriction enzymes?

    <p>To mark specific sequences in DNA for recognition by restriction enzymes.</p> Signup and view all the answers

    What is indicated by the three-letter code assigned to restriction enzymes?

    <p>The organism from which the enzyme was isolated.</p> Signup and view all the answers

    How do restriction enzymes contribute to the formation of recombinant DNA constructs?

    <p>By recognizing and cleaving specific DNA sequences to allow new fragment joining.</p> Signup and view all the answers

    What does it mean when a DNA fragment has blunt ends?

    <p>It lacks any exposed single-stranded regions.</p> Signup and view all the answers

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