General Study Notes Overview

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Questions and Answers

What is genomic imprinting?

Epigenetic silencing of one X chromosome in females.

Modification of DNA sequences through environmental factors.

Heritable changes in gene expression based on the parent of origin. (correct)

A type of recombinant DNA technology application.

Transgenerational effects through paramutation involve modifications to the DNA sequence.

False

Name one application of recombinant DNA technology.

Gene Therapy

In female mammals, one X chromosome is epigenetically silenced in each cell to balance gene expression with ______.

males Signup and view all the answers

Match the following concepts with their definitions:

Restriction endonucleases = Enzymes that cut DNA at specific sites. DNA methylation patterns = Chemical modifications that can affect gene expression. Histone acetylation = Modification that increases gene expression. Recombinant DNA = DNA formed by combining genetic material from different sources. Signup and view all the answers

What is the role of insulators in relation to enhancers?

Insulators block interaction between enhancers and promoters. Signup and view all the answers

Repressors bind to enhancer regions to increase transcription.

False Signup and view all the answers

What are the different functions provided by protein domains?

DNA binding, activation, interaction with other proteins Signup and view all the answers

The process that produces different mRNA isoforms from the same gene is called ______.

alternative splicing Signup and view all the answers

Match the RNA types to their characteristics:

miRNA = Endogenous, imperfect base pairing, represses translation siRNA = Exogenous, perfect base pairing, cleaves target mRNA sncRNA = Regulates gene expression post-transcriptionally lncRNA = Regulates chromatin state and transcription Signup and view all the answers

What initiates the recruitment of RNA polymerase II for transcription?

Formation of a pre-initiation complex with general transcription factors Signup and view all the answers

Nonsense-mediated decay targets mRNAs with premature stop codons.

True Signup and view all the answers

SncRNAs are processed by ______ into small fragments.

Dicer Signup and view all the answers

Which label is used to visualize specific DNA/RNA sequences in chromosomes or cells?

Fluorescent label Signup and view all the answers

The main difference between a Southern blot and a Northern blot is that Southern blots analyze RNA while Northern blots analyze DNA.

False Signup and view all the answers

What technology uses chain-terminating dideoxynucleotides to determine DNA sequences?

Sanger Sequencing Signup and view all the answers

FISH stands for __________ in situ hybridization.

fluorescence Signup and view all the answers

Match the following genetic engineering techniques with their descriptions:

Knock-out (KO) = Delete part of a gene causing loss-of-function mutation Conditional KO = Induces KO only under specific conditions Transgenic or Knock-In (KI) = Alters gene sequence to change the gene CRISPR = Engineered nuclease technology for genome editing Signup and view all the answers

What is one application of DNA sequencing?

Paternity tests Signup and view all the answers

The Cre-lox system allows for the precise control of gene knockouts in specific tissues.

True Signup and view all the answers

Name one of the engineered nucleases used in genetic engineering.

CRISPR Signup and view all the answers

What gene is primarily responsible for determining maleness?

SRY gene Signup and view all the answers

Individuals with Klinefelter syndrome have an extra Y chromosome.

False Signup and view all the answers

What happens to the gonadal ridges in the presence of a Y chromosome?

They develop into testes. Signup and view all the answers

Individuals with a karyotype of 47, XXY are diagnosed with __________ syndrome.

Klinefelter Signup and view all the answers

Match the following terms with their descriptions:

SRY gene = Determines male development Klinefelter syndrome = 47, XXY karyotype Turner syndrome = 45, X0 karyotype Pseudoautosomal regions = Allow X and Y recombination Signup and view all the answers

Which of the following describes sexual dimorphism?

The differences between male and female traits beyond reproduction Signup and view all the answers

All human embryos start as distinctly male or female until differentiation occurs.

False Signup and view all the answers

What is the primary role of the hormones secreted by testes during sexual differentiation?

To drive male sexual differentiation and inhibit female reproductive organ development. Signup and view all the answers

What is one of the key modifications scientists made to the CRISPR-Cas9 system for their in vitro research?

They combined tracrRNA and crRNA into a single guide RNA (gRNA). Signup and view all the answers

CRISPR-Cas9 is less efficient than previous genome editing technologies.

False Signup and view all the answers

What are the two main repair mechanisms that CRISPR-Cas9 utilizes?

Non-Homologous End Joining (NHEJ) and Homology-Directed Repair (HDR). Signup and view all the answers

The CRISPR-Cas9 system requires a ____ protein adapted for eukaryotic conditions.

Cas9 Signup and view all the answers

Match the following aspects of CRISPR-Cas9 with their purposes:

Cas9 Protein = Bacterial protein adapted for eukaryotic conditions gRNA = Custom-designed to target DNA Delivery System = Methods for gene insertion Repair Template = Used for homology-directed repair (HDR) Signup and view all the answers

Which of the following is NOT a characteristic of Non-Homologous End Joining (NHEJ)?

Requires a repair template Signup and view all the answers

Homology-Directed Repair (HDR) is quicker than Non-Homologous End Joining (NHEJ).

False Signup and view all the answers

Name one advancement made by scientists to improve the CRISPR-Cas9 system for precise editing.

Engineered Cas9 variants with nickase activity. Signup and view all the answers

What is the primary function of the crRNA in the CRISPR-Cas system?

To guide Cas proteins to target DNA Signup and view all the answers

The CRISPR system cannot remember previous viral infections.

False Signup and view all the answers

Name one bacterial species associated with Type I CRISPR-Cas systems.

E. coli Signup and view all the answers

The PAM sequence is necessary for Cas9 to recognize and bind to the DNA, specifically it is a sequence of _____ in the form of 5'-NGG-3'.

nucleotides Signup and view all the answers

Match the type of CRISPR-Cas system to its associated characteristic:

Type I = Involves Cascade complex Type II = Used for genome editing with Cas9 Type III = Contains Cas10 complex Signup and view all the answers

What are the three main steps of the CRISPR-Cas mechanism?

Spacer Acquisition, crRNA Biogenesis, Target Interference Signup and view all the answers

TracrRNA is important for connecting crRNA to Cas9.

True Signup and view all the answers

What is the role of the crRNA in targeting foreign DNA?

It contains a sequence complementary to the target DNA. Signup and view all the answers

Study Notes