Podcast
Questions and Answers
Which staining method is used to detect acid-fast bacteria?
Which staining method is used to detect acid-fast bacteria?
- Ziehl-Neelsen staining (correct)
- Negative staining
- Simple staining
- Gram's staining
What is the primary function of pili in a bacterial cell?
What is the primary function of pili in a bacterial cell?
- Protection from desiccation
- DNA transfer (correct)
- Cellular movement
- Metabolic processes
Which chemical agent is commonly used for disinfection of medical equipment?
Which chemical agent is commonly used for disinfection of medical equipment?
- Dyes
- Ethylene oxide (correct)
- Alcohol
- Halogens
What is the primary purpose of using agar in culture media?
What is the primary purpose of using agar in culture media?
Which physical agent is commonly used for sterilization in the food industry?
Which physical agent is commonly used for sterilization in the food industry?
Flashcards
Acid-fast bacteria stain
Acid-fast bacteria stain
A method of staining bacteria that resist decolorization by acid-alcohol.
Pili function in bacteria
Pili function in bacteria
Facilitating genetic transfer via conjugation.
Ethylene oxide use
Ethylene oxide use
A powerful sterilant used for medical equipment disinfection.
Agar's role in culture media
Agar's role in culture media
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Food industry sterilization agent
Food industry sterilization agent
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Study Notes
Staining Method for Acid-Fast Bacteria
- The Ziehl-Neelsen stain is commonly used to identify acid-fast bacteria, particularly Mycobacterium species.
- This staining method highlights the resistance of these bacteria to decolorization by acids.
Function of Pili in Bacterial Cells
- Pili, also known as fimbriae, facilitate adhesion of bacteria to surfaces or other cells.
- They play a crucial role in the formation of biofilms and can assist in genetic exchange through conjugation.
Chemical Agent for Disinfection of Medical Equipment
- Glutaraldehyde is frequently employed as a disinfecting agent for medical instruments and equipment.
- It is effective against a broad range of bacteria, viruses, and fungi due to its potent aldehyde properties.
Purpose of Agar in Culture Media
- Agar serves as a gelatinous base in culture media, providing a stable environment for microbial growth.
- It allows for the differential culture of microorganisms by enabling the isolation of specific species while inhibiting others.
Physical Agent for Sterilization in the Food Industry
- High-temperature steam, known as autoclaving, is widely used for sterilization in food processing.
- This method ensures the destruction of microbial life by applying heat and pressure, preserving food quality and safety.
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