General Microbiology Quiz

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Questions and Answers

Which staining method is used to detect acid-fast bacteria?

  • Ziehl-Neelsen staining (correct)
  • Negative staining
  • Simple staining
  • Gram's staining

What is the primary function of pili in a bacterial cell?

  • Protection from desiccation
  • DNA transfer (correct)
  • Cellular movement
  • Metabolic processes

Which chemical agent is commonly used for disinfection of medical equipment?

  • Dyes
  • Ethylene oxide (correct)
  • Alcohol
  • Halogens

What is the primary purpose of using agar in culture media?

<p>To provide nutrients for bacterial growth (A)</p> Signup and view all the answers

Which physical agent is commonly used for sterilization in the food industry?

<p>Irradiation (D)</p> Signup and view all the answers

Flashcards

Acid-fast bacteria stain

A method of staining bacteria that resist decolorization by acid-alcohol.

Pili function in bacteria

Facilitating genetic transfer via conjugation.

Ethylene oxide use

A powerful sterilant used for medical equipment disinfection.

Agar's role in culture media

A solid substance to create a growing surface for bacteria.

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Food industry sterilization agent

Irradiation is used for food preservation and sterilization.

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Study Notes

Staining Method for Acid-Fast Bacteria

  • The Ziehl-Neelsen stain is commonly used to identify acid-fast bacteria, particularly Mycobacterium species.
  • This staining method highlights the resistance of these bacteria to decolorization by acids.

Function of Pili in Bacterial Cells

  • Pili, also known as fimbriae, facilitate adhesion of bacteria to surfaces or other cells.
  • They play a crucial role in the formation of biofilms and can assist in genetic exchange through conjugation.

Chemical Agent for Disinfection of Medical Equipment

  • Glutaraldehyde is frequently employed as a disinfecting agent for medical instruments and equipment.
  • It is effective against a broad range of bacteria, viruses, and fungi due to its potent aldehyde properties.

Purpose of Agar in Culture Media

  • Agar serves as a gelatinous base in culture media, providing a stable environment for microbial growth.
  • It allows for the differential culture of microorganisms by enabling the isolation of specific species while inhibiting others.

Physical Agent for Sterilization in the Food Industry

  • High-temperature steam, known as autoclaving, is widely used for sterilization in food processing.
  • This method ensures the destruction of microbial life by applying heat and pressure, preserving food quality and safety.

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