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Questions and Answers
Please identify each of the following as an atom, an element, an ion, a molecule, or a compound:
H2O = Molecule, Compound NH4+ = Ion NH3 = Molecule, Compound H2 = Molecule C6H12O6 = Molecule, Compound H3O+ = Ion F– = Ion Na = Element Ar = Element O22– = Ion
What is an atom?
The smallest particle or unit of an element.
What is a molecule?
A single structure made of two or more atoms.
What is a compound?
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What are diatomic elements?
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What type of compounds are made of a metal and a nonmetal?
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What is the name of the acid for HCl?
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What are polyatomic ions?
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The SI unit for length is the ______.
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The prefix 'Kilo-' represents ______.
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List one example of a strong acid.
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What is an allotrope?
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How many seconds are in a day?
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How many hours are in a year?
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How many kg are there in 7×10^25 μg?
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How many seconds are there in 5.2 × 10^18 ns?
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Please convert 20 km to cm.
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If you’re going 50 miles/hour, how many feet/second are you traveling?
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On average, hummingbirds fly at 30 miles/hour. Convert this speed to m/s.
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The Brooklyn Bridge is 277 feet tall. Convert this to meters.
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In a vacuum, light travels at a speed of 2.998 x 10^8 m/s. Convert this to miles per hour.
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What is the mass in kilograms of 18.5 gallons of gasoline, given that the density of gasoline is 0.70 g/mL?
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What is the density, in g/cm³, of a 1.5 kg cube whose edges are each 5 cm?
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After carrying out the following operation, the reported value should have how many significant figures? (6.943 cm – 5.81 cm) × 3.23 cm
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Which measurement below contains 3 significant figures?
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Which of the following has the same number of significant figures as the number 1.00310?
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What is the main knowledge gap addressed in the scientific article?
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What is the research question of the study?
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Which of the following increases levels of microbial contamination in makeup products? (Select all that apply)
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What analyses were used to address the research question?
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Beauty blenders were found to be the least contaminated makeup product.
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How were the results of the study displayed?
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What methods did the researchers suggest to avoid significant levels of contamination?
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The results of the study did not support the initial hypothesis.
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What did the authors cite to connect bacteria to makeup contamination?
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What important feature helps in identifying bacterial species?
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Which of the following is NOT a benefit of bacteria to humans?
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Which arrangement describes the cells of Streptococcus pyogenes?
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What is the primary reason for using a microscope in the study of microorganisms?
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Which of the following describes the nature of viruses in relation to the domains Eukarya and Bacteria?
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Which type of microscopy allows for the visualization of living specimens and intracellular structures without killing the organisms?
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What is the primary advantage of fluorescence microscopy in medical diagnostics?
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What distinguishes transmission electron microscopy (TEM) from scanning electron microscopy (SEM)?
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What happens to living organisms when they are affixed to slides for microscopy?
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What is the role of fluorochrome dye in fluorescence microscopy?
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What is the primary function of the iris diaphragm in a bright light microscope?
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Which of the following describes a binocular light microscope?
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What is the purpose of the objective lenses in a compound light microscope?
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Which of the following objective lenses provides the highest magnification?
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Where is the light source located in a bright light microscope?
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What function does the condenser serve in a bright light microscope?
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In a monocular light microscope, what characteristic does it have?
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What is the function of placing oil on the slide before using the 100X objective?
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What factor does NOT influence the resolution of a microscope?
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What is the highest resolution achievable with a bright light microscope?
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Which type of microscope is particularly useful for studying unstained, living specimens?
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What is the approximate cost of microscopes used in introductory microbiology laboratories?
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How does the numerical aperture of a lens influence microscopy?
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What happens to light as it passes from air into glass in microscopy?
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Which microscope type would NOT be ideal for bright field microscopy?
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Which statement about the bright light microscope is true?
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What technique is employed in dark-field microscopy?
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Study Notes
Atoms, Ions, and Molecules
- An atom is the smallest unit of an element.
- Ions are charged atoms formed by the addition or removal of electrons (e.g., H+ is a cation; H– is an anion).
- A molecule consists of two or more bonded atoms, which can be identical or different elements.
- A compound is a specific type of molecule that contains at least two different elements (e.g., H2O).
Classification of Chemical Species
- H2O: Both a molecule and a compound.
- NH4+: Ion (specifically a cation).
- NH3: Molecule (can also be categorized as a compound).
- H2: Molecule (specifically a diatomic molecule).
- C6H12O6: Molecule and compound.
- H3O+: Ion (specifically a cation).
- F–: Ion (specifically an anion).
- Na: Element (monatomic).
- Ar: Element (monatomic).
- O22–: Ion (specifically an anion).
Elements and Compounds
- Monatomic elements are single atoms of an element (e.g., Na, Cs).
- Diatomic elements exist as pairs of atoms (e.g., H2, N2, O2, F2, Cl2, Br2, I2).
- Allotropes are different structural forms of the same element (e.g., O2 and O3 for oxygen).
Types of Compounds
- Ionic compounds: Formed from metals and nonmetals (e.g., NaCl, CuCl2). NH4Cl is an exception that is ionic but lacks metals.
- Molecular compounds: Formed from two or more nonmetals (e.g., H2O, CO2).
Naming Ionic Compounds
- Name the metal followed by its oxidation state in Roman numerals.
- No Roman numerals for specific metals (e.g., Group I and II metals, Al, Zn, Cd, Ag).
- Nonmetals are named with an “ide” ending.
Polyatomic Ions
- Cations: NH4+
- Anions: Include acetate (CH3COO−), sulfate (SO42−), carbonate (CO32−), hydroxide (HO−), nitrate (NO3−), phosphate (PO43−), permanganate (MnO4−).
Naming Molecular Compounds
- Use numerical prefixes to denote the number of atoms (e.g., Mono, Di, Tri).
- Name the first element followed by the second with an “ide” ending.
Naming Acids
- Binary acids: Start with "hydro-" plus the second element's name ending in “ic acid” (e.g., HF - Hydrofluoric acid).
-
Oxyacids: Named based on the number of oxygen atoms present relative to a root element:
- Hypo-: Fewest oxygen atoms.
- -ous: One fewer than the most.
- -ic: Most oxygen atoms.
- Per-: One more than the most.
Strong Acids to Memorize
- Binary Acids: HCl, HBr, HI.
- Oxyacids: HClO3, HClO4, HNO3, H2SO4.
SI Units
- Common SI units include:
- Length: Meter (m)
- Time: Second (s)
- Temperature: Kelvin (K)
- Mass: Kilogram (kg)
- Mole: Amount of substance (mol)
- Electric current: Ampere (A)
Unit Prefixes
- Common prefixes:
- Tera (10^12), Giga (10^9), Mega (10^6), Kilo (10^3), Centi (10^-2), Milli (10^-3), Micro (10^-6), Nano (10^-9).
Dimensional Analysis Steps
- Write down the value you want to convert.
- Create parentheses for the conversion factors.
- Ensure units in the numerator and denominator cancel appropriately.
- Multiply out and simplify to achieve the desired units with appropriate significant figures.
Example Conversion
- To convert 8.41 × 10^6 L to gallons:
- Set up the conversion factors with L in the denominator and gal in the numerator.
- Use the known relationship 1 gal = 3.785 L to fill in numbers.
- Calculate to find the volume in gallons.### Unit Conversion and Calculations
- Ensure parentheses are correctly used in calculations to achieve a true statement.
- When multiplying units, cancel out starting units to reach desired ones.
- Significant figures must be applied in results, especially when calculations are done without a calculator.
Density and Conversion
- Density is defined as mass divided by volume, crucial for converting between mass and volume.
Time and Distance Questions
- Know conversions for time: seconds in a day (86,400 sec), hours in a year (approximately 8,760 hr).
- Understand mass conversions: 1 kg = 1,000,000 µg, and conversions between miles and kilometers (1 mile = 1.61 km).
- Be able to convert between different units of distance, such as kilometers to centimeters (1 km = 100,000 cm).
Significant Figures
- Significant figures indicate the precision of measurements; count from the first non-zero digit.
- Rules for significant figures:
- Zeroes between non-zero digits are significant (e.g., 1005 kg has four figures).
- Leading zeroes (e.g., 0.02 g) are not significant.
- Trailing zeroes are significant only if there is a decimal point (e.g., 0.0200 g has three figures).
Arithmetic with Significant Figures
- For addition/subtraction, the result should match the decimal places of the least precise measurement.
- For multiplication/division, the result should match the smallest number of significant figures among the values.
Precision vs. Accuracy
- Precision relates to the closeness of repeated measurements.
- Accuracy represents how close a measurement is to the actual value.
Percent Error
- Percent error compares the theoretical value to the actual measure and is expressed as:
- % error = ((actual - theoretical) / theoretical) × 100
Lab Safety
- Wear personal protective equipment (PPE) in the lab including lab coats, goggles, and gloves.
- Common sense should guide safety practices, avoiding risky behaviors.
Test Tube Heating
- Use appropriate holders to heat test tubes safely; avoid open flames when possible.
- Always add acid to water for dilution to prevent exothermic reactions causing spattering.
Weighing Reagents
- Always weigh reagents on weigh paper; tare the paper first for accurate measurements.
- Avoid contamination by not transferring reagents directly from containers into weigh paper.
Measuring Liquids
- Read liquid volumes at the bottom of the meniscus in graduated cylinders.
- Parallax errors occur if the measurement is viewed from an incorrect angle.
pH Meter Usage
- Calibrate the pH probe with standard buffers before use.
- Rinse and dry the probe between samples for accurate measurements.
Introduction
- Investigates levels of microbial contaminants in various makeup products.
- Research question: Assess microbial contamination in lipstick, lip gloss, eyeliners, mascaras, and beauty blenders, and associated health risks.
- Main arguments: Dropping products, using unsanitary or expired items elevates contamination risks in makeup.
Materials and Methods
- Analyzed 100 microliters from each makeup product type, inoculated on various media: NA, MSA, SMAC, SAB, VRBGA.
- Emphasized accurate measurements for product quantities and inoculation procedures.
- Two tables and two figures utilized to support data presentation and methodology.
Results
- Beauty blenders showed the highest level of microbial contamination among tested products.
- Significant microbial growth detected across all tested makeup products, presenting potential health risks.
- Results were illustrated through tables and figures, complemented by detailed explanations in the results and discussion sections.
- Figure captions clarified the comparison and information displayed, while table captions provided brief explanations of table contents.
Discussion
- Discussed specific bacterial types identified in contaminated makeup and related health concerns.
- Suggested prevention methods to mitigate contamination: avoiding makeup testers and not sharing products.
- Findings validated the initial hypothesis regarding contamination levels.
- Cited previous research connecting bacteria types and the importance of prior studies on makeup contamination reduction.
- Reiterated main findings and their implications on public health, reinforcing the need for consumer awareness regarding makeup safety practices.
Bacterial Species and Their Impact
- A small percentage of bacteria cause clinical diseases in humans.
- Bacteria play crucial roles in bioremediation, nutrient cycling, and producing substances like insulin.
- "Germs" is a common term that oversimplifies the diversity of microorganisms.
Microorganisms and Their Classification
- Microorganisms display various traits, such as morphology and movement, similar to human differences.
- Representative members of Eukarya and Bacteria will be studied, along with nonliving viruses.
- Understanding morphology and arrangements is essential for microbial identification.
Types of Bacterial Morphologies
- Bacteria typically exhibit three shapes: coccus (circular), bacillus (rod), and spirillum (spiral).
- Arrangement examples:
- Streptococcus pyogenes: chain-like (coccus).
- Staphylococcus aureus: grape-like clusters (coccus).
Microscopy Basics
- Observing microorganisms requires a microscope due to their small size.
- A bright light microscope uses visible light, with multiple objective lenses for varying magnification.
- Monocular microscope has one eyepiece; binocular microscope has two.
Operable Components of a Microscope
- Light passes from the base through a condenser onto the specimen held on a mechanical stage.
- An iris diaphragm controls light intensity; four objective lenses magnify the specimen at different levels (4X, 10X, 40X, and 100X).
- The 100X objective requires oil immersion to enhance clarity due to light refraction.
Understanding Resolution and Magnification
- Resolution is the ability to distinguish between two objects; optimal is approximately 0.2 μm with bright light microscopes.
- Electron microscopes provide higher resolution due to shorter electron wavelengths, surpassing light microscope limitations.
Specialized Microscopy Techniques
- Dark-field microscopy allows visualization of unstained living specimens against a dark background.
- Phase-contrast microscopy enhances visibility of cellular organelles and motility in living specimens.
- Fluorescence microscopy uses fluorochrome dyes to detect specific microorganisms by glowing under ultraviolet light.
Electron Microscopy Types
- Transmission Electron Microscope (TEM): Visualizes internal structures by directing electrons through the specimen.
- Scanning Electron Microscope (SEM): Highlights surface details using scattered electrons.
Microscope Care and Handling
- Always transport microscopes with care; support both the arm and base and keep it close to the body.
- Clean lenses before use with lens paper and 70% ethanol to maintain efficiency.
- Observing slides should involve both eyes open to reduce eye strain.
Post-Use Procedures
- After completing lab work, remove slides, clean lenses, and return the microscope to its designated position.
- Properly store the microscope with the arm facing forward in the cabinet to ensure safety and longevity.
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Description
This quiz offers a quick review of fundamental chemistry concepts, focusing on atoms, ions, and molecules. Explore the characteristics of these particles, how ions are formed, and their significance in chemical reactions.