Gene Structure and Expression Quiz
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Questions and Answers

What distinguishes the transcription process in prokaryotes from eukaryotes?

  • Eukaryotes utilize RNA polymerase I, II, and III. (correct)
  • Eukaryotes have operons that group related genes.
  • Prokaryotes use ribosomes to initiate transcription.
  • Transcription occurs in the nucleus in prokaryotes.
  • Which correctly describes how transcription is initiated in eukaryotes?

  • Transcription begins automatically without any proteins.
  • Ribosomes directly bind to DNA to start transcription.
  • Promoter regions are not involved in transcription initiation.
  • Transcription factors bind to the promoter region. (correct)
  • Which statement accurately describes the function of RNA polymerases in eukaryotes?

  • They play different roles in transcribing various types of RNA. (correct)
  • They are exclusively responsible for synthesizing ribosomal RNA.
  • They can only synthesize RNA when bound to ribosomal proteins.
  • They can synthesize both DNA and RNA strands.
  • What must happen during transcription mechanisms before RNA is synthesized?

    <p>The DNA strand must be unwound and a complementary RNA strand formed.</p> Signup and view all the answers

    How is gene organization typically structured in prokaryotes?

    <p>Genes are organized into operons for related functions.</p> Signup and view all the answers

    What is the relationship between genes and enzymes as observed in Beadle and Tatum's experiments?

    <p>Genes directly encode proteins that catalyze reactions.</p> Signup and view all the answers

    What evidence did Garrod provide regarding the link between genetics and metabolic disorders?

    <p>Identified that certain metabolic disorders are inherited and linked to gene alterations.</p> Signup and view all the answers

    Which statement best explains the effect of X-rays in Beadle and Tatum's study on Neurospora crassa?

    <p>X-rays induced mutagenesis, impacting specific genes encoding enzymes.</p> Signup and view all the answers

    What was the purpose of comparing wild type and auxotrophic mutants in Beadle and Tatum's research?

    <p>To identify the steps in the arginine synthesis pathway and their gene associations.</p> Signup and view all the answers

    Which conclusion can be drawn from the argF and ArgE mutants' behavior in the arginine synthesis pathway?

    <p>Each mutant has a unique defect in the enzyme synthesis path.</p> Signup and view all the answers

    What is the primary function of the promoter in a gene?

    <p>To initiate transcription by providing a binding site for RNA polymerase</p> Signup and view all the answers

    How do prokaryotic and eukaryotic transcription processes differ regarding RNA polymerase binding?

    <p>Eukaryotic RNA polymerase requires transcription factors to bind to DNA</p> Signup and view all the answers

    Which codon serves as the start codon for translation?

    <p>AUG</p> Signup and view all the answers

    What is the role of RNA polymerase during transcription?

    <p>To add RNA nucleotides sequentially according to the DNA template</p> Signup and view all the answers

    What defines the termination of transcription in a gene?

    <p>The terminator signals the end of transcription</p> Signup and view all the answers

    How does the genetic code's universality affect coding for amino acids?

    <p>The same codons specify the same amino acids across all organisms</p> Signup and view all the answers

    What does redundancy in the genetic code refer to?

    <p>Some amino acids are specified by multiple different codons</p> Signup and view all the answers

    What is the direction of RNA synthesis during transcription?

    <p>5’ to 3’ direction</p> Signup and view all the answers

    Which of the following is NOT a stop codon?

    <p>AUG</p> Signup and view all the answers

    Study Notes

    Gene Structure and Expression

    • Genes provide instructions for building proteins through transcription and translation.
    • Proteins carry out many cellular functions.

    How Genes Encode Proteins

    • Garrod (1896) studied alkaptonuria, a metabolic disorder, linking it to a gene alteration.

    • Beadle and Tatum (1940s) investigated orange bread mold (Neurospora crassa) to further explore the relationship between genes and enzymes.

    • Their hypothesis suggested a specific gene for each enzyme involved in a metabolic pathway.

    • Garrod's work demonstrated that alterations in genes result in metabolic disorders, and that these disorders are often inherited.

    • Beadle and Tatum's experiments supported that a single gene, codes for a single enzyme.

    Beadle and Tatum Experiment

    • The experiment utilized orange bread mold (Neurospora crassa).
    • Each step in a metabolic pathway (e.g., arginine synthesis) is regulated by a gene that encodes the specific enzyme.

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    Description

    Test your knowledge on gene structure and the process of protein expression. This quiz covers key experiments by Garrod and Beadle & Tatum, illustrating how genes encode proteins and the implications for metabolic disorders. Explore how these foundational studies shaped our understanding of genetics.

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