25 Questions
What prevents transcription in negative regulation of gene expression?
Bound repressor protein
What activates transcription in the Lac operon when lactose is present?
Bound activator protein
What senses low levels of glucose and activates the Lac operon?
Catabolite Activating Protein (CAP)
What is needed to establish interactions between different components in Eukaryotic gene control?
DNA looping
Which type of proteins bind mostly as complexes rather than single proteins in Eukaryotic gene regulation?
Mediator proteins
What is the main purpose of Chromatin Remodeling Complex?
To open up chromatin structure
What is the role of Histone Deacetylase in gene expression regulation?
It removes acetylation groups from histones
What is the function of a Histonemodifyingcomplex?
Modifying histone proteins
What does the recruitment of Histone methy transferase result in?
Chromatin compaction
Why is Chromatin Remodeling Complex important for gene expression?
To regulate chromatin accessibility
What does Chromatin immunoprecipitation aim to show?
The location where a protein specifically binds to a chromosome
What is the purpose of adding a cross-linking agent like formaldehyde in Chromatin immunoprecipitation?
To freeze the protein-DNA interaction in living cells
What is a key difference between Prokaryotic Gene Expression and Eukaryotic Gene Expression?
Prokaryotic genes are polycistronic, while Eukaryotic genes are monocistronic
What is the main purpose of an operon in Prokaryotic Gene Expression?
To coordinate the expression of multiple genes involved in a related function
Why does Eukaryotic Gene Expression involve switching genes ON or OFF?
To regulate the expression of single genes to respond to different cellular needs
What is the main difference between how cells differentiate gene expression?
Protein patterns
Which process is used to separate proteins based on their charge or molecular weight?
Gel electrophoresis
In Eukaryotic gene expression, mRNA degradation control is a mechanism for regulating gene expression by:
Facilitating mRNA breakdown
In prokaryotic gene expression, what is the role of the promoter region?
Binding to transcription factors
What is the main function of DNA-binding proteins in transcriptional regulation?
Regulating transcription initiation
What kind of proteins bind to the surface of DNA without unwinding it?
Transcription factors
Which motif is known for mediating dimerization in DNA binding proteins?
Leucine zipper
What kind of assay detects sequence-specific DNA binding proteins?
Gel mobility shift assay
What type of DNA will migrate quickly through a gel in the gel mobility shift assay?
Negatively charged DNA
What does DNase footprinting determine about DNA binding proteins?
Sequence specificity
Test your knowledge on gene regulation and the lac operon. Learn about the mechanisms of negative and positive regulation, as well as how the lac operon controls the breakdown of lactose into glucose and galactose.
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