Gene Regulation and Lac Operon Quiz
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Questions and Answers

What prevents transcription in negative regulation of gene expression?

  • Activated repressor protein
  • Bound activator protein
  • Catabolite Activating Protein (CAP)
  • Bound repressor protein (correct)
  • What activates transcription in the Lac operon when lactose is present?

  • Activated repressor protein
  • Catabolite Activating Protein (CAP)
  • Bound activator protein (correct)
  • Cleavage of glucose
  • What senses low levels of glucose and activates the Lac operon?

  • Mediator protein complex
  • Activated repressor protein
  • Bound repressor protein
  • Catabolite Activating Protein (CAP) (correct)
  • What is needed to establish interactions between different components in Eukaryotic gene control?

    <p>DNA looping</p> Signup and view all the answers

    Which type of proteins bind mostly as complexes rather than single proteins in Eukaryotic gene regulation?

    <p>Mediator proteins</p> Signup and view all the answers

    What is the main purpose of Chromatin Remodeling Complex?

    <p>To open up chromatin structure</p> Signup and view all the answers

    What is the role of Histone Deacetylase in gene expression regulation?

    <p>It removes acetylation groups from histones</p> Signup and view all the answers

    What is the function of a Histonemodifyingcomplex?

    <p>Modifying histone proteins</p> Signup and view all the answers

    What does the recruitment of Histone methy transferase result in?

    <p>Chromatin compaction</p> Signup and view all the answers

    Why is Chromatin Remodeling Complex important for gene expression?

    <p>To regulate chromatin accessibility</p> Signup and view all the answers

    What does Chromatin immunoprecipitation aim to show?

    <p>The location where a protein specifically binds to a chromosome</p> Signup and view all the answers

    What is the purpose of adding a cross-linking agent like formaldehyde in Chromatin immunoprecipitation?

    <p>To freeze the protein-DNA interaction in living cells</p> Signup and view all the answers

    What is a key difference between Prokaryotic Gene Expression and Eukaryotic Gene Expression?

    <p>Prokaryotic genes are polycistronic, while Eukaryotic genes are monocistronic</p> Signup and view all the answers

    What is the main purpose of an operon in Prokaryotic Gene Expression?

    <p>To coordinate the expression of multiple genes involved in a related function</p> Signup and view all the answers

    Why does Eukaryotic Gene Expression involve switching genes ON or OFF?

    <p>To regulate the expression of single genes to respond to different cellular needs</p> Signup and view all the answers

    What is the main difference between how cells differentiate gene expression?

    <p>Protein patterns</p> Signup and view all the answers

    Which process is used to separate proteins based on their charge or molecular weight?

    <p>Gel electrophoresis</p> Signup and view all the answers

    In Eukaryotic gene expression, mRNA degradation control is a mechanism for regulating gene expression by:

    <p>Facilitating mRNA breakdown</p> Signup and view all the answers

    In prokaryotic gene expression, what is the role of the promoter region?

    <p>Binding to transcription factors</p> Signup and view all the answers

    What is the main function of DNA-binding proteins in transcriptional regulation?

    <p>Regulating transcription initiation</p> Signup and view all the answers

    What kind of proteins bind to the surface of DNA without unwinding it?

    <p>Transcription factors</p> Signup and view all the answers

    Which motif is known for mediating dimerization in DNA binding proteins?

    <p>Leucine zipper</p> Signup and view all the answers

    What kind of assay detects sequence-specific DNA binding proteins?

    <p>Gel mobility shift assay</p> Signup and view all the answers

    What type of DNA will migrate quickly through a gel in the gel mobility shift assay?

    <p>Negatively charged DNA</p> Signup and view all the answers

    What does DNase footprinting determine about DNA binding proteins?

    <p>Sequence specificity</p> Signup and view all the answers

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