Gene Regulation and Lac Operon Quiz

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Questions and Answers

What prevents transcription in negative regulation of gene expression?

  • Activated repressor protein
  • Bound activator protein
  • Catabolite Activating Protein (CAP)
  • Bound repressor protein (correct)

What activates transcription in the Lac operon when lactose is present?

  • Activated repressor protein
  • Catabolite Activating Protein (CAP)
  • Bound activator protein (correct)
  • Cleavage of glucose

What senses low levels of glucose and activates the Lac operon?

  • Mediator protein complex
  • Activated repressor protein
  • Bound repressor protein
  • Catabolite Activating Protein (CAP) (correct)

What is needed to establish interactions between different components in Eukaryotic gene control?

<p>DNA looping (A)</p> Signup and view all the answers

Which type of proteins bind mostly as complexes rather than single proteins in Eukaryotic gene regulation?

<p>Mediator proteins (A)</p> Signup and view all the answers

What is the main purpose of Chromatin Remodeling Complex?

<p>To open up chromatin structure (B)</p> Signup and view all the answers

What is the role of Histone Deacetylase in gene expression regulation?

<p>It removes acetylation groups from histones (C)</p> Signup and view all the answers

What is the function of a Histonemodifyingcomplex?

<p>Modifying histone proteins (C)</p> Signup and view all the answers

What does the recruitment of Histone methy transferase result in?

<p>Chromatin compaction (C)</p> Signup and view all the answers

Why is Chromatin Remodeling Complex important for gene expression?

<p>To regulate chromatin accessibility (D)</p> Signup and view all the answers

What does Chromatin immunoprecipitation aim to show?

<p>The location where a protein specifically binds to a chromosome (D)</p> Signup and view all the answers

What is the purpose of adding a cross-linking agent like formaldehyde in Chromatin immunoprecipitation?

<p>To freeze the protein-DNA interaction in living cells (A)</p> Signup and view all the answers

What is a key difference between Prokaryotic Gene Expression and Eukaryotic Gene Expression?

<p>Prokaryotic genes are polycistronic, while Eukaryotic genes are monocistronic (D)</p> Signup and view all the answers

What is the main purpose of an operon in Prokaryotic Gene Expression?

<p>To coordinate the expression of multiple genes involved in a related function (A)</p> Signup and view all the answers

Why does Eukaryotic Gene Expression involve switching genes ON or OFF?

<p>To regulate the expression of single genes to respond to different cellular needs (C)</p> Signup and view all the answers

What is the main difference between how cells differentiate gene expression?

<p>Protein patterns (A)</p> Signup and view all the answers

Which process is used to separate proteins based on their charge or molecular weight?

<p>Gel electrophoresis (A)</p> Signup and view all the answers

In Eukaryotic gene expression, mRNA degradation control is a mechanism for regulating gene expression by:

<p>Facilitating mRNA breakdown (A)</p> Signup and view all the answers

In prokaryotic gene expression, what is the role of the promoter region?

<p>Binding to transcription factors (A)</p> Signup and view all the answers

What is the main function of DNA-binding proteins in transcriptional regulation?

<p>Regulating transcription initiation (A)</p> Signup and view all the answers

What kind of proteins bind to the surface of DNA without unwinding it?

<p>Transcription factors (B)</p> Signup and view all the answers

Which motif is known for mediating dimerization in DNA binding proteins?

<p>Leucine zipper (B)</p> Signup and view all the answers

What kind of assay detects sequence-specific DNA binding proteins?

<p>Gel mobility shift assay (A)</p> Signup and view all the answers

What type of DNA will migrate quickly through a gel in the gel mobility shift assay?

<p>Negatively charged DNA (D)</p> Signup and view all the answers

What does DNase footprinting determine about DNA binding proteins?

<p>Sequence specificity (B)</p> Signup and view all the answers

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