Gene Expression Regulation in Prokaryotes and Eukaryotes

A mutant that is always on, expressing the lac genes regardless of lactose or glucose levels.

A group of genes that are transcribed together as a single mRNA.

A process where the expression of a gene depends on which parent it originated from.

A bacterial defense system that can be used to precisely target and edit genes.

Negative control silences gene expression. Positive control activates it. Both are ways to regulate genes.

Experiments that use DNAase to reveal the structure of chromatin, showing which parts are open or closed to transcription.

A mutant carrying two copies of the lac operon, showing regulation is dependent on repressor proteins and operator sequences.

High glucose levels repress the lac operon, because the cell prefers glucose as its energy source.

A technique used to isolate bacterial mutants based on their inability to grow under specific conditions. It involves transferring colonies from one plate to another using a sterile velvet pad.

A mutant gene that is always expressed, regardless of the presence of its inducer or repressor. It's like a switch that's always on.

Positive control activates gene expression in the presence of a specific molecule, while negative control inhibits gene expression in the presence of a repressor.

DNA sequences that can regulate gene expression by binding to transcription factors. They can be located far away from the promoter and can enhance gene transcription.