5 Questions
What is the function of transfer RNA (tRNA) in translation?
Carries a specific amino acid and has an anticodon for mRNA recognition
What is the role of the codon chart in gene expression?
It maps mRNA codons to specific amino acids
What is the primary function of organic extraction in RNA isolation?
Removing proteins, lipids, and DNA from the RNA sample
Which statement accurately describes Trizol reagent in RNA isolation?
It is a mono-phasic solution that lyses cells and dissolves cell components while maintaining RNA integrity
What is the role of ribosomes in gene expression?
They facilitate the translation of mRNA codons into specific amino acid sequences
Study Notes
Translation and Gene Expression
- Transfer RNA (tRNA) brings amino acids to the ribosome to be incorporated into a growing polypeptide chain during protein synthesis.
RNA Isolation
- Organic extraction is used to separate RNA from other cellular components, such as proteins and DNA, to obtain a pure RNA sample.
- Trizol reagent is a monophasic solution of phenol and guanidine isothiocyanate that denatures proteins and solubilizes lipids, allowing for the separation of RNA from other cellular components.
Codon Chart
- A codon chart, also known as the genetic code table, is a reference guide that shows how nucleotide triplets (codons) correspond to specific amino acids during translation.
Ribosomes
- Ribosomes are the site of protein synthesis, where mRNA is translated into a polypeptide chain, and they play a central role in gene expression.
Test your knowledge of gene expression and translation processes, including the relationship between mRNA codons and amino acids, and the role of ribosomes and tRNA molecules in translation.
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