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Questions and Answers
What is the function of transfer RNA (tRNA) in translation?
What is the function of transfer RNA (tRNA) in translation?
- Provides the template for protein synthesis
- Facilitates the formation of peptide bonds between amino acids
- Carries a specific amino acid and has an anticodon for mRNA recognition (correct)
- Transcribes DNA into mRNA for translation
What is the role of the codon chart in gene expression?
What is the role of the codon chart in gene expression?
- It maps mRNA codons to specific amino acids (correct)
- It regulates the expression of genes in the cell
- It assists in the transcription of tRNA molecules
- It determines the sequence of DNA nucleotides
What is the primary function of organic extraction in RNA isolation?
What is the primary function of organic extraction in RNA isolation?
- Enhancing the stability of RNA molecules
- Removing proteins, lipids, and DNA from the RNA sample (correct)
- Amplifying the RNA for downstream applications
- Converting RNA into complementary DNA (cDNA)
Which statement accurately describes Trizol reagent in RNA isolation?
Which statement accurately describes Trizol reagent in RNA isolation?
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What is the role of ribosomes in gene expression?
What is the role of ribosomes in gene expression?
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Study Notes
Translation and Gene Expression
- Transfer RNA (tRNA) brings amino acids to the ribosome to be incorporated into a growing polypeptide chain during protein synthesis.
RNA Isolation
- Organic extraction is used to separate RNA from other cellular components, such as proteins and DNA, to obtain a pure RNA sample.
- Trizol reagent is a monophasic solution of phenol and guanidine isothiocyanate that denatures proteins and solubilizes lipids, allowing for the separation of RNA from other cellular components.
Codon Chart
- A codon chart, also known as the genetic code table, is a reference guide that shows how nucleotide triplets (codons) correspond to specific amino acids during translation.
Ribosomes
- Ribosomes are the site of protein synthesis, where mRNA is translated into a polypeptide chain, and they play a central role in gene expression.
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