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Questions and Answers
What is the purpose of the Lac Z gene in the blue-white screening method?
What is the purpose of the Lac Z gene in the blue-white screening method?
- To produce the beta galactosidase enzyme for converting X-Gal into a blue compound (correct)
- To prevent the growth of bacterial colonies
- To allow the growth of bacterial colonies
- To disrupt the formation of the X-Gal compound
Why are plasmids used in gene cloning?
Why are plasmids used in gene cloning?
- They contain defined copy numbers that are highly regulated within a cell (correct)
- They disrupt the formation of beta galactosidase enzyme
- They contain a gene for producing X-Gal compound
- They prevent the growth of bacterial colonies
What is the role of the antibiotic resistance gene in plasmids?
What is the role of the antibiotic resistance gene in plasmids?
- It disrupts the production of beta galactosidase enzyme
- It allows cells containing the plasmid to grow in the presence of the antibiotic within the growth medium (correct)
- It inhibits the ligation process
- It prevents the transformation of recombinant DNA into bacterial cells
What is the function of restriction enzymes in gene cloning?
What is the function of restriction enzymes in gene cloning?
What is the purpose of the multiple cloning sites (MCS) in cloning plasmids?
What is the purpose of the multiple cloning sites (MCS) in cloning plasmids?
What is the purpose of transformation in gene cloning?
What is the purpose of transformation in gene cloning?
What method is used to reintroduce recombinant DNA into bacterial cells?
What method is used to reintroduce recombinant DNA into bacterial cells?
Why is blue-white screening used in gene cloning?
Why is blue-white screening used in gene cloning?
What is the main purpose of gene cloning?
What is the main purpose of gene cloning?
What is the role of a vector in gene cloning?
What is the role of a vector in gene cloning?
What is a vector containing foreign DNA called?
What is a vector containing foreign DNA called?
What are the characteristics of suitable vectors for gene cloning?
What are the characteristics of suitable vectors for gene cloning?
How can appropriate cells containing the vector be selected?
How can appropriate cells containing the vector be selected?
Why is it beneficial to have a way to detect cells containing only the recombinant DNA?
Why is it beneficial to have a way to detect cells containing only the recombinant DNA?
Why is gene cloning useful in further studying the function of a gene or protein?
Why is gene cloning useful in further studying the function of a gene or protein?
Sticky ends are less efficient for ligation compared to blunt ends in gene cloning.
Sticky ends are less efficient for ligation compared to blunt ends in gene cloning.
The Lac Z gene disruption causes bacterial colonies to turn blue in the blue-white screening method.
The Lac Z gene disruption causes bacterial colonies to turn blue in the blue-white screening method.
The antibiotic resistance gene in plasmids allows bacteria to survive in the presence of antibiotics.
The antibiotic resistance gene in plasmids allows bacteria to survive in the presence of antibiotics.
The process of gene cloning involves cutting the plasmid DNA, ligating foreign DNA, transforming the recombinant plasmid into bacteria, and then selecting for the presence of the recombinant plasmid.
The process of gene cloning involves cutting the plasmid DNA, ligating foreign DNA, transforming the recombinant plasmid into bacteria, and then selecting for the presence of the recombinant plasmid.
The blue-white screening method allows all colonies to be white if they contain the recombinant DNA.
The blue-white screening method allows all colonies to be white if they contain the recombinant DNA.
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