Gene Cloning and Its Applications
20 Questions
3 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the purpose of the Lac Z gene in the blue-white screening method?

  • To produce the beta galactosidase enzyme for converting X-Gal into a blue compound (correct)
  • To prevent the growth of bacterial colonies
  • To allow the growth of bacterial colonies
  • To disrupt the formation of the X-Gal compound

    • Why are plasmids used in gene cloning?

  • They contain defined copy numbers that are highly regulated within a cell (correct)
  • They disrupt the formation of beta galactosidase enzyme
  • They contain a gene for producing X-Gal compound
  • They prevent the growth of bacterial colonies

    • What is the role of the antibiotic resistance gene in plasmids?

  • It disrupts the production of beta galactosidase enzyme
  • It allows cells containing the plasmid to grow in the presence of the antibiotic within the growth medium (correct)
  • It inhibits the ligation process
  • It prevents the transformation of recombinant DNA into bacterial cells

    • What is the function of restriction enzymes in gene cloning?

    <p>They induce double-stranded cuts in DNA at specific sequences</p> Signup and view all the answers

    What is the purpose of the multiple cloning sites (MCS) in cloning plasmids?

    <p>To provide a group of unique restriction sites for inserting foreign DNA</p> Signup and view all the answers

    What is the purpose of transformation in gene cloning?

    <p>To introduce recombinant DNA into bacterial cells</p> Signup and view all the answers

    What method is used to reintroduce recombinant DNA into bacterial cells?

    <p>Heat shock</p> Signup and view all the answers

    Why is blue-white screening used in gene cloning?

    <p>To distinguish between non-recombinant and recombinant clones based on beta galactosidase activity</p> Signup and view all the answers

    What is the main purpose of gene cloning?

    <p>All of the above</p> Signup and view all the answers

    What is the role of a vector in gene cloning?

    <p>To carry the genetic material into another cell for replication or expression</p> Signup and view all the answers

    What is a vector containing foreign DNA called?

    <p>Recombinant DNA</p> Signup and view all the answers

    What are the characteristics of suitable vectors for gene cloning?

    <p>Independent from host chromosome, copied by host cell, maintaining the recombinant vector throughout the population of cells, allow for selection of cells containing the vector</p> Signup and view all the answers

    How can appropriate cells containing the vector be selected?

    <p>Antibiotic resistance for cells containing the vector and nutritional selection for those containing the recombinant vector</p> Signup and view all the answers

    Why is it beneficial to have a way to detect cells containing only the recombinant DNA?

    <p>To allow for selection of cells with specific genetic material</p> Signup and view all the answers

    Why is gene cloning useful in further studying the function of a gene or protein?

    <p>To make many identical copies of DNA to study gene or protein function</p> Signup and view all the answers

    Sticky ends are less efficient for ligation compared to blunt ends in gene cloning.

    <p>False</p> Signup and view all the answers

    The Lac Z gene disruption causes bacterial colonies to turn blue in the blue-white screening method.

    <p>False</p> Signup and view all the answers

    The antibiotic resistance gene in plasmids allows bacteria to survive in the presence of antibiotics.

    <p>True</p> Signup and view all the answers

    The process of gene cloning involves cutting the plasmid DNA, ligating foreign DNA, transforming the recombinant plasmid into bacteria, and then selecting for the presence of the recombinant plasmid.

    <p>True</p> Signup and view all the answers

    The blue-white screening method allows all colonies to be white if they contain the recombinant DNA.

    <p>False</p> Signup and view all the answers

    More Like This

    Biotechnology Principles and Processes Quiz
    10 questions

    Biotechnology Principles and Processes Quiz: Test Your Knowledge

    LogicalSynthesizer avatar
    LogicalSynthesizer
    Gene Cloning
    6 questions

    Gene Cloning

    LuminousOrientalism avatar
    LuminousOrientalism
    Recombinant DNA Technology Overview
    10 questions

    Recombinant DNA Technology Overview

    ExceptionalTheme avatar
    ExceptionalTheme
    Recombinant DNA Technology and Gene Cloning
    40 questions

    Recombinant DNA Technology and Gene Cloning

    HilariousMelodica avatar
    HilariousMelodica
    Use Quizgecko on...
    Browser
    Open
    Browser
    Browser