24 Questions
What is the purpose of the carrier gas in gas-liquid chromatography (GLC)?
To carry the sample components through the apparatus
Which gases are commonly used as carrier gases in gas-liquid chromatography (GLC)?
Helium, nitrogen, argon, hydrogen, carbon dioxide
How are the sample components separated in gas-liquid chromatography (GLC)?
As they pass through the column
What is the function of the stationary phase in gas-liquid chromatography (GLC)?
To separate the sample components
What is the purpose of the rubber stopper at the top of the column?
To introduce the sample at the top of the column
What is the importance of keeping the injection point unit at a higher temperature than the rest of the column?
To ensure immediate evaporation of the liquid sample once it enters the column
What are the two distinct types of columns commonly used in chromatography?
Packed and open tubular (capillary)
What are the characteristics of an ideal solid support for chromatography?
High specific surface, chemically inert, and wettable by the liquid phase
What is the purpose of the soap film flow-meter in chromatography?
To measure the flow rate of the sample
Why is it important for the injection point unit to be kept at a higher temperature than the rest of the column?
To ensure immediate evaporation of the liquid sample
What is the function of the rubber stopper at the top of the column?
To introduce the sample at the top of the column
What are the characteristics of an ideal solid support for chromatography?
High specific surface and chemically inert
What is the function of the carrier gas in gas-liquid chromatography (GLC)?
To provide transportation for the sample components through the apparatus
What are the most commonly used gases as carrier gases in gas-liquid chromatography (GLC)?
Helium, nitrogen, argon, hydrogen
Why is it important for the stationary phase to be a non-volatile liquid in gas-liquid chromatography (GLC)?
To ensure the sample components are not carried away by the carrier gas
What is the purpose of the thermo stated oven in gas-liquid chromatography (GLC)?
To provide controlled temperature for the column, injector, and detector
What is the function of the soap film flow-meter in gas-liquid chromatography (GLC)?
To measure the flow rate of the carrier gas as it passes through the column
Why is it important for the stationary phase to be a non-volatile liquid in gas-liquid chromatography (GLC)?
To prevent evaporation of the stationary phase and ensure its stability
What are the main characteristics of an ideal solid support for gas-liquid chromatography (GLC)?
Chemically inert and high surface area
Why is a thermo stated oven provided for the column, injector, and detector in gas-liquid chromatography (GLC)?
To maintain a constant temperature for efficient separation and detection
What is the purpose of the rubber stopper at the top of the column in chromatography?
To create a seal for introducing the sample at the top of the column
Why is it important for the injection point unit to be kept at a higher temperature than the rest of the column in chromatography?
To ensure the immediate evaporation of the liquid sample
What is the function of the soap film flow-meter in chromatography?
To measure the flow rate
What are the characteristics of an ideal solid support for chromatography?
High specific surface, chemically inert, wetable surface
Learn about gas-liquid chromatography, a partition type of chromatography involving a liquid stationary phase and a gas mobile phase. Understand the process of sample injection and separation in the column.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
