Gas-Liquid Chromatography (GLC) Lecture 9
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Questions and Answers

What is the purpose of the carrier gas in gas-liquid chromatography (GLC)?

  • To carry the sample components through the apparatus (correct)
  • To exhaust the gas to the atmosphere
  • To pack the column with a porous solid
  • To heat the column, injector, and detector

    • Which gases are commonly used as carrier gases in gas-liquid chromatography (GLC)?

  • Nitrous oxide, sulfur dioxide, chlorine, ammonia, hydrogen sulfide
  • Helium, nitrogen, argon, hydrogen, carbon dioxide (correct)
  • Oxygen, krypton, neon, radon, xenon
  • Methane, ethane, propane, butane, pentane

    • How are the sample components separated in gas-liquid chromatography (GLC)?

  • As they pass through the column (correct)
  • By heating the column
  • As they pass through the mixing chamber
  • By passing through a flow meter

    • What is the function of the stationary phase in gas-liquid chromatography (GLC)?

    <p>To separate the sample components</p> Signup and view all the answers

    What is the purpose of the rubber stopper at the top of the column?

    <p>To introduce the sample at the top of the column</p> Signup and view all the answers

    What is the importance of keeping the injection point unit at a higher temperature than the rest of the column?

    <p>To ensure immediate evaporation of the liquid sample once it enters the column</p> Signup and view all the answers

    What are the two distinct types of columns commonly used in chromatography?

    <p>Packed and open tubular (capillary)</p> Signup and view all the answers

    What are the characteristics of an ideal solid support for chromatography?

    <p>High specific surface, chemically inert, and wettable by the liquid phase</p> Signup and view all the answers

    What is the purpose of the soap film flow-meter in chromatography?

    <p>To measure the flow rate of the sample</p> Signup and view all the answers

    Why is it important for the injection point unit to be kept at a higher temperature than the rest of the column?

    <p>To ensure immediate evaporation of the liquid sample</p> Signup and view all the answers

    What is the function of the rubber stopper at the top of the column?

    <p>To introduce the sample at the top of the column</p> Signup and view all the answers

    What are the characteristics of an ideal solid support for chromatography?

    <p>High specific surface and chemically inert</p> Signup and view all the answers

    What is the function of the carrier gas in gas-liquid chromatography (GLC)?

    <p>To provide transportation for the sample components through the apparatus</p> Signup and view all the answers

    What are the most commonly used gases as carrier gases in gas-liquid chromatography (GLC)?

    <p>Helium, nitrogen, argon, hydrogen</p> Signup and view all the answers

    Why is it important for the stationary phase to be a non-volatile liquid in gas-liquid chromatography (GLC)?

    <p>To ensure the sample components are not carried away by the carrier gas</p> Signup and view all the answers

    What is the purpose of the thermo stated oven in gas-liquid chromatography (GLC)?

    <p>To provide controlled temperature for the column, injector, and detector</p> Signup and view all the answers

    What is the function of the soap film flow-meter in gas-liquid chromatography (GLC)?

    <p>To measure the flow rate of the carrier gas as it passes through the column</p> Signup and view all the answers

    Why is it important for the stationary phase to be a non-volatile liquid in gas-liquid chromatography (GLC)?

    <p>To prevent evaporation of the stationary phase and ensure its stability</p> Signup and view all the answers

    What are the main characteristics of an ideal solid support for gas-liquid chromatography (GLC)?

    <p>Chemically inert and high surface area</p> Signup and view all the answers

    Why is a thermo stated oven provided for the column, injector, and detector in gas-liquid chromatography (GLC)?

    <p>To maintain a constant temperature for efficient separation and detection</p> Signup and view all the answers

    What is the purpose of the rubber stopper at the top of the column in chromatography?

    <p>To create a seal for introducing the sample at the top of the column</p> Signup and view all the answers

    Why is it important for the injection point unit to be kept at a higher temperature than the rest of the column in chromatography?

    <p>To ensure the immediate evaporation of the liquid sample</p> Signup and view all the answers

    What is the function of the soap film flow-meter in chromatography?

    <p>To measure the flow rate</p> Signup and view all the answers

    What are the characteristics of an ideal solid support for chromatography?

    <p>High specific surface, chemically inert, wetable surface</p> Signup and view all the answers

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