FmlH and UTI Pathogenesis

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What is the role of FmlH in UTI pathogenesis?

FmlH is involved in UTI pathogenesis.

What strategy was used to develop high-affinity galactoside antagonists of FmlH?

An X-ray structure-guided medicinal chemistry approach was used.

What was the first step in developing galactoside antagonists of FmlH?

Screening a select library of galactosides through multiplex ELISA arrays for initial lead compound identification.

What type of assay was developed to detect binding in the competition assay?

An ELISA-based competition assay was developed. Signup and view all the answers

What glycan receptors does FmlH bind to?

FmlH binds to surface glycan receptors containing terminal Gal, GalNAc, or TF residues. Signup and view all the answers

What approach was used to identify the early lead inhibitor of the F9 Pilus Adhesin FmlH?

Multiplex ELISA arrays were used to screen a select library of galactosides for initial lead compound identification. Signup and view all the answers

What was the iterative process involved in developing high-affinity galactoside antagonists of FmlH?

The iterative process involved cocrystal structure determination, virtual screening, structure-based ligand design, and in vitro biochemical characterization. Signup and view all the answers

What was the purpose of evaluating the top lead compound in a mouse model of UTI?

The purpose was to evaluate the efficacy of the lead compound in a relevant infection model. Signup and view all the answers

What was the goal of adapting Gal, GalNAc, and TF to function as soluble, competitive inhibitors of FmlH?

The goal was to develop galactoside antagonists that could competitively inhibit the binding of FmlH to surface glycan receptors. Signup and view all the answers

What type of assay was developed to detect binding in the competition assay?

An ELISA-based competition assay was developed to detect binding. Signup and view all the answers

FmlH and UTI Pathogenesis

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Questions and Answers

What is the role of FmlH in UTI pathogenesis?

What strategy was used to develop high-affinity galactoside antagonists of FmlH?

What was the first step in developing galactoside antagonists of FmlH?

What type of assay was developed to detect binding in the competition assay?

What glycan receptors does FmlH bind to?

What approach was used to identify the early lead inhibitor of the F9 Pilus Adhesin FmlH?

What was the iterative process involved in developing high-affinity galactoside antagonists of FmlH?

What was the purpose of evaluating the top lead compound in a mouse model of UTI?

What was the goal of adapting Gal, GalNAc, and TF to function as soluble, competitive inhibitors of FmlH?

What type of assay was developed to detect binding in the competition assay?

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