FmlH and UTI Pathogenesis
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Questions and Answers

What is the role of FmlH in UTI pathogenesis?

FmlH is involved in UTI pathogenesis.

What strategy was used to develop high-affinity galactoside antagonists of FmlH?

An X-ray structure-guided medicinal chemistry approach was used.

What was the first step in developing galactoside antagonists of FmlH?

Screening a select library of galactosides through multiplex ELISA arrays for initial lead compound identification.

What type of assay was developed to detect binding in the competition assay?

<p>An ELISA-based competition assay was developed.</p> Signup and view all the answers

What glycan receptors does FmlH bind to?

<p>FmlH binds to surface glycan receptors containing terminal Gal, GalNAc, or TF residues.</p> Signup and view all the answers

What approach was used to identify the early lead inhibitor of the F9 Pilus Adhesin FmlH?

<p>Multiplex ELISA arrays were used to screen a select library of galactosides for initial lead compound identification.</p> Signup and view all the answers

What was the iterative process involved in developing high-affinity galactoside antagonists of FmlH?

<p>The iterative process involved cocrystal structure determination, virtual screening, structure-based ligand design, and in vitro biochemical characterization.</p> Signup and view all the answers

What was the purpose of evaluating the top lead compound in a mouse model of UTI?

<p>The purpose was to evaluate the efficacy of the lead compound in a relevant infection model.</p> Signup and view all the answers

What was the goal of adapting Gal, GalNAc, and TF to function as soluble, competitive inhibitors of FmlH?

<p>The goal was to develop galactoside antagonists that could competitively inhibit the binding of FmlH to surface glycan receptors.</p> Signup and view all the answers

What type of assay was developed to detect binding in the competition assay?

<p>An ELISA-based competition assay was developed to detect binding.</p> Signup and view all the answers

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