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Questions and Answers
What is the most crucial step in gram staining?
Which component of the Gram stain is a dry fixative that strengthens the affinity between the cell wall and the stain?
What provides rigidity to the cell wall in gram-positive organisms by attracting magnesium and calcium?
Why do Mycoplasma, Ureaplasma, and Acholeplasma usually show a gram-negative reaction despite not having a gram-negative cell wall?
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Why are spirals (spirochetes) difficult to stain using gram staining?
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What is the primary reason Mycobacterium and Nocardia spp. are difficult to perform a gram stain on?
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Which staining method is typically used for Mycobacterium and Nocardia spp. due to their acid-fast nature?
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In the context of gram staining, what is the significance of gram variability in gram-positive bacteria?
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What is the purpose of acid-fast staining?
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Which component is part of the Acid-Fast Stain (FHAM)?
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What happens to acid-fast bacteria during the decolorization step with Acid Alcohol?
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Which bacterium is known as a partially acid-fast organism?
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In the Ziehl-Neelsen Method, what is the primary stain used?
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What effect does the presence of mycolic acids have on acid-fast bacteria?
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