Fecal Concentration Techniques

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Questions and Answers

What is the advantage of using ethyl acetate in the centrifugation technique described?

  • Skims parasites from the top of the tube
  • Concentrates oocyst of Cryptosporidium
  • Improves the recovery of most parasites (correct)
  • Reduces fecal debris in the preparation

Why is zinc sulfate centrifugal flotation technique preferred over other methods?

  • Removes more fecal debris from the preparation (correct)
  • Is recommended for G.lamblia cyst & H.nana ova
  • Skims parasites from the top of the tube
  • Allows direct mixing with stool without centrifugation

What is a disadvantage of using zinc sulfate centrifugal flotation technique?

  • Floats Isospora oocysts effectively
  • May miss some helminth eggs that are very dense (correct)
  • Leads to shrunken Hookworm eggs
  • Does not require centrifugation step

Why is Sheather's flotation method recommended for certain parasite types?

<p>Preserves oocysts of Cryptosporidium effectively (B)</p> Signup and view all the answers

Which type of eggs are not suited for Brine's flotation method?

<p>Trematode eggs (C)</p> Signup and view all the answers

Why might some helminth eggs be missed using the zinc sulfate centrifugal flotation method?

<p>'Density' issue of helminth eggs (A)</p> Signup and view all the answers

What is the purpose of concentrating feces?

<p>To decrease the amount of background material in the preparation (A)</p> Signup and view all the answers

What should be done before proceeding to fecal concentration?

<p>Direct examination (A)</p> Signup and view all the answers

What is a key difference between concentrated preparations and direct microscopy?

<p>Motile protozoan trophozoites are not found in concentrated preparations (B)</p> Signup and view all the answers

Why do parasites settle at the bottom of the tube in the sedimentation method?

<p>They have a higher specific gravity (C)</p> Signup and view all the answers

Which concentration technique is the method of choice if the specimen is from an animal source?

<p>Acid ether concentration technique (D)</p> Signup and view all the answers

Why is HCl used in the acid ether concentration technique?

<p>As a clearing agent (B)</p> Signup and view all the answers

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