Fecal Concentration Techniques

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What is the advantage of using ethyl acetate in the centrifugation technique described?

Improves the recovery of most parasites

Why is zinc sulfate centrifugal flotation technique preferred over other methods?

Removes more fecal debris from the preparation

What is a disadvantage of using zinc sulfate centrifugal flotation technique?

May miss some helminth eggs that are very dense

Why is Sheather's flotation method recommended for certain parasite types?

Preserves oocysts of Cryptosporidium effectively

Which type of eggs are not suited for Brine's flotation method?

Trematode eggs

Why might some helminth eggs be missed using the zinc sulfate centrifugal flotation method?

'Density' issue of helminth eggs

What is the purpose of concentrating feces?

To decrease the amount of background material in the preparation

What should be done before proceeding to fecal concentration?

Direct examination

What is a key difference between concentrated preparations and direct microscopy?

Motile protozoan trophozoites are not found in concentrated preparations

Why do parasites settle at the bottom of the tube in the sedimentation method?

They have a higher specific gravity

Which concentration technique is the method of choice if the specimen is from an animal source?

Acid ether concentration technique

Why is HCl used in the acid ether concentration technique?

As a clearing agent

Learn about the purpose of concentrating feces to increase the chances of finding protozoan cysts, helminth eggs, and larvae. Understand the importance of direct examination before using concentration techniques and how they help detect more organisms compared to direct microscopy.

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