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12 Questions

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Fecal Concentration Techniques

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Questions and Answers

What is the advantage of using ethyl acetate in the centrifugation technique described?

Skims parasites from the top of the tube

Concentrates oocyst of Cryptosporidium

Improves the recovery of most parasites (correct)

Reduces fecal debris in the preparation

Why is zinc sulfate centrifugal flotation technique preferred over other methods?

Removes more fecal debris from the preparation (correct)

Is recommended for G.lamblia cyst & H.nana ova

Skims parasites from the top of the tube

Allows direct mixing with stool without centrifugation

What is a disadvantage of using zinc sulfate centrifugal flotation technique?

Floats Isospora oocysts effectively

May miss some helminth eggs that are very dense (correct)

Leads to shrunken Hookworm eggs

Does not require centrifugation step

Why is Sheather's flotation method recommended for certain parasite types?

Preserves oocysts of Cryptosporidium effectively Signup and view all the answers

Which type of eggs are not suited for Brine's flotation method?

Trematode eggs Signup and view all the answers

Why might some helminth eggs be missed using the zinc sulfate centrifugal flotation method?

'Density' issue of helminth eggs Signup and view all the answers

What is the purpose of concentrating feces?

To decrease the amount of background material in the preparation Signup and view all the answers

What should be done before proceeding to fecal concentration?

Direct examination Signup and view all the answers

What is a key difference between concentrated preparations and direct microscopy?

Motile protozoan trophozoites are not found in concentrated preparations Signup and view all the answers

Why do parasites settle at the bottom of the tube in the sedimentation method?

They have a higher specific gravity Signup and view all the answers

Which concentration technique is the method of choice if the specimen is from an animal source?

Acid ether concentration technique Signup and view all the answers

Why is HCl used in the acid ether concentration technique?

As a clearing agent Signup and view all the answers