Expression System Selection and Protein Production
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Questions and Answers

Which organism is preferred for the expression of large proteins (>100 kD)?

  • Fungi
  • Bacteria
  • Viruses
  • Eukaryotes (correct)
  • What is the problem in plants with the beta-carotene pathway?

  • Lack of ξ-carotene desaturase
  • Lack of phytoene synthase (correct)
  • Lack of lycopene-beta-cyclase
  • Lack of geranylgeranyl diphosphate
  • What is the solution to the problem of rice lacking enzymes in the beta-carotene pathway?

  • Adding phytoene desaturase
  • Adding ξ-carotene desaturase
  • Adding daffodil gene (correct)
  • Adding lycopene-beta-cyclase
  • What is the main purpose of developing Golden Rice?

    <p>To reduce childhood malnutrition</p> Signup and view all the answers

    Which organizations developed Golden Rice?

    <p>Both DA-PhilRice and IRRI</p> Signup and view all the answers

    What are some benefits of genetically modified crops?

    <p>All of the above</p> Signup and view all the answers

    What is the role of the daffodil gene in Golden Rice?

    <p>It performs both phytoene desaturase and ξ-carotene desaturase functions</p> Signup and view all the answers

    What is beta-carotene converted into by the body?

    <p>Vitamin A</p> Signup and view all the answers

    What is the name given to rice enriched with additional levels of beta-carotene?

    <p>Golden Rice</p> Signup and view all the answers

    Who will be the first to cultivate Golden Rice?

    <p>Farmers in the Philippines</p> Signup and view all the answers

    Which of the following benefits can be attributed to genetically modified animals?

    <p>Increased resistance, productivity, hardiness, and feed efficiency</p> Signup and view all the answers

    What is one potential human health impact associated with genetically modified products?

    <p>Allergens transfer</p> Signup and view all the answers

    Which of the following is a potential environmental impact of genetically modified products?

    <p>Loss of flora and fauna biodiversity</p> Signup and view all the answers

    What is one benefit of genetically modified animals for society?

    <p>Increased food security for growing populations</p> Signup and view all the answers

    What is one potential concern associated with genetically modified products?

    <p>Unintended transfer of transgenes through crosspollination</p> Signup and view all the answers

    Which of the following is a benefit of genetically modified products for the environment?

    <p>Conservation of soil, water, and energy</p> Signup and view all the answers

    What is one potential impact of genetically modified products on access and intellectual property?

    <p>Biopiracy—foreign exploitation of natural resources</p> Signup and view all the answers

    Which of the following is a potential concern associated with genetically modified animals?

    <p>Unknown effects on other organisms (e.g., soil microbes)</p> Signup and view all the answers

    What is one potential benefit of genetically modified products for animals?

    <p>Improved animal health and diagnostic methods</p> Signup and view all the answers

    Which of the following is a potential concern associated with genetically modified products for society?

    <p>Genetically modified products: controversies</p> Signup and view all the answers

    Which type of protein is best suited for expression in a eukaryote?

    <p>Large proteins</p> Signup and view all the answers

    What is the main purpose of developing Golden Rice?

    <p>To reduce childhood malnutrition</p> Signup and view all the answers

    What is the role of the daffodil gene in Golden Rice?

    <p>To produce beta-carotene</p> Signup and view all the answers

    What is the problem in plants with the beta-carotene pathway?

    <p>Lack of enzymes</p> Signup and view all the answers

    Which organizations developed Golden Rice?

    <p>Both DA-PhilRice and IRRI</p> Signup and view all the answers

    What is beta-carotene converted into by the body?

    <p>Vitamin A</p> Signup and view all the answers

    What are some benefits of genetically modified crops?

    <p>All of the above</p> Signup and view all the answers

    Which of the following is a potential concern associated with genetically modified products for society?

    <p>Domination of world food production by a few companies</p> Signup and view all the answers

    What is one potential human health impact associated with genetically modified products?

    <p>Transfer of antibiotic resistance markers</p> Signup and view all the answers

    Which of the following is a potential environmental impact of genetically modified products?

    <p>Unintended transfer of transgenes through crosspollination</p> Signup and view all the answers

    What is one benefit of genetically modified animals for society?

    <p>Increased food security for growing populations</p> Signup and view all the answers

    What is the main purpose of developing Golden Rice?

    <p>Increased food security for growing populations</p> Signup and view all the answers

    What is one potential concern associated with genetically modified animals?

    <p>Transfer of antibiotic resistance markers</p> Signup and view all the answers

    What is one potential benefit of genetically modified products for animals?

    <p>Increased resistance, productivity, hardiness, and feed efficiency</p> Signup and view all the answers

    What is one potential impact of genetically modified products on access and intellectual property?

    <p>Domination of world food production by a few companies</p> Signup and view all the answers

    Which organization developed Golden Rice?

    <p>International Rice Research Institute (IRRI)</p> Signup and view all the answers

    What is the main purpose of developing Golden Rice?

    <p>To reduce childhood malnutrition</p> Signup and view all the answers

    What is the problem in plants with the beta-carotene pathway?

    <p>Lack of enzymes</p> Signup and view all the answers

    Which organism is preferred for the expression of large proteins (>100 kD)?

    <p>Eukaryote</p> Signup and view all the answers

    What is beta-carotene converted into by the body?

    <p>Vitamin A</p> Signup and view all the answers

    What is one potential concern associated with genetically modified products?

    <p>Environmental impact</p> Signup and view all the answers

    What is one potential benefit of genetically modified products for animals?

    <p>Increased nutrients</p> Signup and view all the answers

    Which of the following is a potential concern associated with genetically modified products for society?

    <p>Controversies surrounding genetically modified products</p> Signup and view all the answers

    What is one potential environmental impact of genetically modified products?

    <p>Unintended transfer of transgenes through crosspollination</p> Signup and view all the answers

    What is one potential impact of genetically modified products on access and intellectual property?

    <p>Biopiracy - foreign exploitation of natural resources</p> Signup and view all the answers

    What is one potential human health impact associated with genetically modified products?

    <p>Allergens transfer of antibiotic resistance markers</p> Signup and view all the answers

    What is one benefit of genetically modified animals for society?

    <p>Increased food security for growing populations</p> Signup and view all the answers

    What is the main purpose of developing Golden Rice?

    <p>Increased food security for growing populations</p> Signup and view all the answers

    What is the problem in plants with the beta-carotene pathway?

    <p>Loss of flora and fauna biodiversity</p> Signup and view all the answers

    Which organizations developed Golden Rice?

    <p>International Rice Research Institute (IRRI) and Swiss Federal Institute of Technology (ETH)</p> Signup and view all the answers

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