Expression System Selection and Protein Production

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Which organism is preferred for the expression of large proteins (>100 kD)?

Eukaryotes

What is the problem in plants with the beta-carotene pathway?

Lack of phytoene synthase

What is the solution to the problem of rice lacking enzymes in the beta-carotene pathway?

Adding daffodil gene

What is the main purpose of developing Golden Rice?

To reduce childhood malnutrition

Which organizations developed Golden Rice?

Both DA-PhilRice and IRRI

What are some benefits of genetically modified crops?

All of the above

What is the role of the daffodil gene in Golden Rice?

It performs both phytoene desaturase and ξ-carotene desaturase functions

What is beta-carotene converted into by the body?

Vitamin A

What is the name given to rice enriched with additional levels of beta-carotene?

Golden Rice

Who will be the first to cultivate Golden Rice?

Farmers in the Philippines

Which of the following benefits can be attributed to genetically modified animals?

Increased resistance, productivity, hardiness, and feed efficiency

What is one potential human health impact associated with genetically modified products?

Allergens transfer

Which of the following is a potential environmental impact of genetically modified products?

Loss of flora and fauna biodiversity

What is one benefit of genetically modified animals for society?

Increased food security for growing populations

What is one potential concern associated with genetically modified products?

Unintended transfer of transgenes through crosspollination

Which of the following is a benefit of genetically modified products for the environment?

Conservation of soil, water, and energy

What is one potential impact of genetically modified products on access and intellectual property?

Biopiracy—foreign exploitation of natural resources

Which of the following is a potential concern associated with genetically modified animals?

Unknown effects on other organisms (e.g., soil microbes)

What is one potential benefit of genetically modified products for animals?

Improved animal health and diagnostic methods

Which of the following is a potential concern associated with genetically modified products for society?

Genetically modified products: controversies

Which type of protein is best suited for expression in a eukaryote?

Large proteins

What is the main purpose of developing Golden Rice?

To reduce childhood malnutrition

What is the role of the daffodil gene in Golden Rice?

To produce beta-carotene

What is the problem in plants with the beta-carotene pathway?

Lack of enzymes

Which organizations developed Golden Rice?

Both DA-PhilRice and IRRI

What is beta-carotene converted into by the body?

Vitamin A

What are some benefits of genetically modified crops?

All of the above

Which of the following is a potential concern associated with genetically modified products for society?

Domination of world food production by a few companies

What is one potential human health impact associated with genetically modified products?

Transfer of antibiotic resistance markers

Which of the following is a potential environmental impact of genetically modified products?

Unintended transfer of transgenes through crosspollination

What is one benefit of genetically modified animals for society?

Increased food security for growing populations

What is the main purpose of developing Golden Rice?

Increased food security for growing populations

What is one potential concern associated with genetically modified animals?

Transfer of antibiotic resistance markers

What is one potential benefit of genetically modified products for animals?

Increased resistance, productivity, hardiness, and feed efficiency

What is one potential impact of genetically modified products on access and intellectual property?

Domination of world food production by a few companies

Which organization developed Golden Rice?

International Rice Research Institute (IRRI)

What is the main purpose of developing Golden Rice?

To reduce childhood malnutrition

What is the problem in plants with the beta-carotene pathway?

Lack of enzymes

Which organism is preferred for the expression of large proteins (>100 kD)?

Eukaryote

What is beta-carotene converted into by the body?

Vitamin A

What is one potential concern associated with genetically modified products?

Environmental impact

What is one potential benefit of genetically modified products for animals?

Increased nutrients

Which of the following is a potential concern associated with genetically modified products for society?

Controversies surrounding genetically modified products

What is one potential environmental impact of genetically modified products?

Unintended transfer of transgenes through crosspollination

What is one potential impact of genetically modified products on access and intellectual property?

Biopiracy - foreign exploitation of natural resources

What is one potential human health impact associated with genetically modified products?

Allergens transfer of antibiotic resistance markers

What is one benefit of genetically modified animals for society?

Increased food security for growing populations

What is the main purpose of developing Golden Rice?

Increased food security for growing populations

What is the problem in plants with the beta-carotene pathway?

Loss of flora and fauna biodiversity

Which organizations developed Golden Rice?

International Rice Research Institute (IRRI) and Swiss Federal Institute of Technology (ETH)

Test your knowledge on expression system selection and the factors to consider when choosing the right system for producing proteins of different sizes. Learn about the advantages of using eukaryotes for large proteins and explore the potential of improving vitamin A content in crops. Challenge yourself with questions on enzyme deficiencies in rice and the pathway for producing β-carotene.

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