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Questions and Answers
What is the role of ddNTPs in Sanger sequencing?
What is the role of ddNTPs in Sanger sequencing?
- They enhance the activity of DNA polymerase
- They act as chain-terminating inhibitors (correct)
- They promote the growth of DNA double strand
- They determine the sequence of nucleotides in DNA
What is the structure of a dNTP?
What is the structure of a dNTP?
- Ribose and one less oxygen
- Deoxyribose and two oxygens
- Deoxyribose, base, and triphosphate (correct)
- Ribose, base, and triphosphate
What does ddNTP stand for?
What does ddNTP stand for?
- Dideoxyribonucleotide triphosphate
- Deoxyribonucleoside triphosphate
- Dideoxyribonucleoside triphosphate (correct)
- Deoxyribonucleotide triphosphate
What is the function of DNA polymerase in Sanger sequencing?
What is the function of DNA polymerase in Sanger sequencing?
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What did Frederick Sanger aim to determine with his sequencing method?
What did Frederick Sanger aim to determine with his sequencing method?
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