Exploring the Basics of Sanger Sequencing
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Questions and Answers

What is the role of ddNTPs in Sanger sequencing?

  • They enhance the activity of DNA polymerase
  • They act as chain-terminating inhibitors (correct)
  • They promote the growth of DNA double strand
  • They determine the sequence of nucleotides in DNA

    • What is the structure of a dNTP?

  • Ribose and one less oxygen
  • Deoxyribose and two oxygens
  • Deoxyribose, base, and triphosphate (correct)
  • Ribose, base, and triphosphate

    • What does ddNTP stand for?

  • Dideoxyribonucleotide triphosphate
  • Deoxyribonucleoside triphosphate
  • Dideoxyribonucleoside triphosphate (correct)
  • Deoxyribonucleotide triphosphate

    • What is the function of DNA polymerase in Sanger sequencing?

    <p>To add new bases to a growing DNA strand</p> Signup and view all the answers

    What did Frederick Sanger aim to determine with his sequencing method?

    <p>The sequence of nucleotides in a piece of DNA</p> Signup and view all the answers

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