Explore the World of Interference Filters

Which type of photometer uses optical filters to isolate a narrow wavelength range of spectrum?

What is the purpose of a spectrophotometer?

In which regions of the spectrum does laboratory spectrophotometry typically involve light?

Which type of detector is 200 times more sensitive than the phototube?

What does the term 'blanking the instrument' refer to?

Which check should be performed to ensure the wavelength indicated on the control dial is the actual wavelength of light passed by the monochromator?

According to Beer's Law, which of the following conditions can cause a deviation from linearity of the absorbance versus concentration curve?

Which of the following is NOT a limitation of Beer's Law?

Which component of a spectrophotometer is responsible for measuring the amount of light transmittance?

Which type of light source provides intense radiation of a narrow wavelength?

Which type of filter produces monochromatic light based on the principle of constructive interference of waves?

Which type of filter offers linear dispersion of light over the entire UV and visible range?

Which type of filter gives a non-linear separation of bands and requires several wavelengths to be measured for proper wavelength calibration?

What is the bandpass of a monochromator?

Which type of cuvette is optimal for spectrophotometry due to its plane-parallel optical surfaces and less error from the lens effect, orientation, and refraction?

Which factor affects the sensitivity of spectrophotometry by increasing the sample size?

Which type of detector in spectrophotometry is 200 times more sensitive than a phototube and is used in instruments that are very sensitive to very low light levels?

What is the purpose of a detector in spectrophotometry?

According to Beer's Law, which of the following is true about the relationship between absorbance and concentration?

What is the mathematical relationship between absorbance (A), molar absorptivity (ε), concentration (c), and light path (b) according to Beer's Law?

What is the relationship between wavelength and energy according to Planck's formula?

What is the purpose of using a blank or reference solution in absorption spectroscopy?

Which type of filter offers linear dispersion of light over the entire UV and visible range?

Which type of filter gives a non-linear separation of bands and requires several wavelengths to be measured for proper wavelength calibration?

What is the bandpass of a monochromator?

What factors determine the bandpass of a monochromator?

Which type of detector in spectrophotometry is 200 times more sensitive than a phototube and is used in instruments that are very sensitive to very low light levels?

What is the purpose of a spectrophotometer?

Which check should be performed to ensure the wavelength indicated on the control dial is the actual wavelength of light passed by the monochromator?

Which of the following is the correct definition of photometry?

Which type of instrument is used to indirectly determine the amount of a compound present in a solution by shining a light of a specific wavelength through the solution and measuring how much was absorbed?

What is the mathematical relationship between absorbance (A), molar absorptivity (ε), concentration (c), and light path (b) according to Beer's Law?

Which of the following conditions can cause a deviation from Beer's Law, resulting in variations from linearity of the absorbance versus concentration curve?

Which type of light source in spectrophotometry provides intense radiation of a narrow wavelength?

Which type of filter in spectrophotometry produces monochromatic light based on the principle of constructive interference of waves?

What is the mathematical relationship between absorbance (A), molar absorptivity (ε), concentration (c), and light path (b) according to Beer's Law?

Which type of cuvette is recommended for spectrophotometry due to its plane-parallel optical surfaces and less error from the lens effect, orientation, and refraction?

What is the main advantage of using a photomultiplier tube (PMT) as a detector in spectrophotometry?

Which type of detector in spectrophotometry generates its own electromotive force and does not require an external voltage source?

Which factor affects the sensitivity of spectrophotometry by increasing the sample size?

According to Beer's Law, which of the following is true about the relationship between absorbance and concentration?

What is the mathematical relationship between absorbance (A), molar absorptivity (ε), concentration (c), and light path (b) according to Beer's Law?

What is the purpose of using a blank or reference solution in absorption spectroscopy?

What is the relationship between wavelength and energy according to Planck's formula?

Which of the following statements is true about electrochemical potential?

What is the role of a salt bridge in electrochemistry?

Which metal is more electronegative in the redox couple of Zinc and Copper?

Which of the following is NOT an application of potentiometry?

What is the main component of potentiometric techniques that measures the desired analyte(s)?

Which type of membrane is commonly used in ion-selective electrodes (ISEs)?

What is the purpose of a pO2 electrode in clinical chemistry?

Which of the following best describes the purpose of mass spectrometry?

What does a mass spectrometer measure?

What happens to a molecule that enters a mass spectrometer without a charge?

Which ionization method uses a corona discharge needle to initiate gas phase reactions that ionize compounds through a series of ion molecule reactions?

Which ionization method is considered 'hard' and fragments molecules to pure atoms at the atomic level?

Which ionization method uses a laser energy absorbing matrix to create ions?

Which mass analyzer is most commonly used and allows only ions of a single selected m/z value to pass through to the detector?

Which component is required in all mass spectrometers to prevent ions from colliding with each other or other molecules during analysis?

What is the unfragmented ion called when a molecular ion breaks into smaller pieces?

Which ionization technique in mass spectrometry results in the mass spectrum being dominated by fragment ions?

Which ionization technique in mass spectrometry results in very little fragmentation during the process?

Which of the following statements is true about time of flight (TOF) mass spectrometry?

What is the main purpose of MALDI-TOF in microbiology?

How does the time of flight (TOF) in a mass spectrometer vary with the mass of the ions?

Which type of instrument is commonly used for drug screening applications and can measure large numbers of analytes simultaneously in complex biological matrices?

What is the purpose of the second quadrupole (Q2) in a triple quad instrument?

Which type of mass spectrometry instrument is capable of identifying genetic diseases and diseases with proteins, and is often used in proteomics research?

What is the purpose of an electron multiplier in a mass spectrometer detector?

Which type of chromatography uses gas as the mobile phase?

What is the purpose of the stationary phase in gas-solid chromatography?

What is one disadvantage of gas-liquid chromatography?

Which type of liquid is commonly used as the stationary phase in partition chromatography?

What is the main purpose of affinity chromatography?

What is the most common material used for packing in high-performance liquid chromatography (HPLC)?

Which type of detector is commonly used in high-performance liquid chromatography (HPLC) to monitor the eluate?

Which type of chromatography uses a liquid mobile phase and a solid stationary phase?

Which type of chromatography uses a gas mobile phase and a solid stationary phase?

What is the measure of retention in thin-layer chromatography?

Which type of chromatography is primarily used for qualitative analysis?

Which type of chromatography is considered the gold standard for most testing due to its high sensitivity and specificity?

What is the term used to describe the average time or volume that is required for a chemical to pass through the column in chromatography?

Which phase in chromatography carries the sample components and travels through the system?

What is the purpose of a chromatograph in chromatography?

Which of the following is a clinical application of osmometry?

What is osmolality?

Which physical properties of a solution are measured in osmometry?

Which of the following is NOT a colligative property affected by the addition of a solute to a solvent?

What is the purpose of osmometry in a medical laboratory?

Which of the following is the major contributor to serum osmolality?

What is the formula to calculate serum osmolality in SI units?

Which of the following is NOT a benefit of laboratory automation?

What is the term used to describe the transport of a quantity of analyte or reagent from one specimen reaction into and contaminating a subsequent one?

Which type of chemistry analyzer uses continuous flow and pumps reagent non-stop through large amounts of plastic tubing?

Which component of automation is responsible for determining which tests need to be performed on a specimen?

Which method of specimen delivery should NOT be used for stat or irreplaceable specimens?

Which phase of handling specimens is one of the most error-prone?

Which type of devices are commonly used for point-of-care testing (POCT)?

What is the disadvantage of using a batch analyzer?

What is the advantage of using a random access analyzer?

What is the purpose of bar-coding in automated analyzers?

What is the purpose of laboratory information systems (LISs)?

Which term describes the overall stability of an antibody-antigen complex, based on affinity, the number of binding sites available, and the way the two molecules combine?

Which type of antigen has the strongest avidity due to having more binding sites?

What is the term used to describe the zone in a precipitation curve where there is an excess of antibodies?

Which type of immunoassay requires a separation step to physically separate free from bound analyte?

In which zone of antigen excess is there a gradual decrease in the amount of precipitation until finally no precipitation is observed?

Which particle method is a passive diffusion method in which a concentration gradient is established for a single reactant, usually the antigen?

Which label method uses radioactive isotopes of iodine and tritium as labels?

Which immunoassay technique uses a chemiluminescent compound to detect analytes?

Which immunoassay technique is a non-competitive sandwich assay?

Which immunoassay technique uses fluorescence to measure the concentration of analytes?

What is the most common error seen in sandwich immunoassays at very high antigen concentrations?