Exophiala Fungi Overview
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Questions and Answers

What temperature condition is specifically required for the growth of Exophiala jeanselmei?

  • 40°C to 42°C
  • 37°C and 40°C
  • 35°C to 37°C
  • Only at 37°C (correct)

Which characteristic distinguishes Exophiala dermatitidis from Exophiala jeanselmei?

  • Shape of conidiophore
  • Color of colonies (correct)
  • Presence of potassium nitrate
  • Growth temperature

What is the shape of the conidia produced by Exophiala species?

  • Rough and irregular
  • Cigar-shaped forms (correct)
  • Elongated and tapered
  • Round and smooth

Which method is useful for detecting melanization in fungal organisms?

<p>Fontana-Masson stain (D)</p> Signup and view all the answers

Which of the following is true for Exophiala dermatitidis?

<p>It can grow at temperatures up to 42°C. (B)</p> Signup and view all the answers

Which characteristic is associated with Exserohilum?

<p>Gray-green to dark-brown colonies (B)</p> Signup and view all the answers

What describes the appearance of Alternaria conidia?

<p>Large brown conidia resembling a drumstick (A)</p> Signup and view all the answers

What is a distinguishing feature of Curvularia?

<p>Golden brown, multi-celled, and curved conidia (C)</p> Signup and view all the answers

Which fungi feature is described as having bent conidiophores?

<p>Bipolaris (D)</p> Signup and view all the answers

Which feature is NOT characteristic of the diagnostic methods for mycoses?

<p>Golden-brown hyphae without septation (D)</p> Signup and view all the answers

Which of the following genera is identified with multiple celled and swollen central cell characteristics?

<p>Curvularia (A)</p> Signup and view all the answers

What feature is crucial in identifying exophiala species?

<p>Gray-green to dark-brown colonies (D)</p> Signup and view all the answers

Which description accurately completes the identification of Bipolaris?

<p>Protruding hilum with germ tubes at both ends (A)</p> Signup and view all the answers

Which species is known for having a cleistothecial structure in its sexual form?

<p>Pseudallescheria boydii (A)</p> Signup and view all the answers

What is the typical characteristic of the conidia produced by Cladophialophora?

<p>Lemoniform to fusiform and slightly verrucous (B)</p> Signup and view all the answers

Which of the following best describes the infection caused by eumycotic mycetomas?

<p>Localized and can lead to chronic granulomatous infection (B)</p> Signup and view all the answers

Which genus is associated with branching, single-celled conidiophores?

<p>Cladophialophora (C)</p> Signup and view all the answers

In which regions are eumycotic mycetomas most commonly found?

<p>Tropical and subtropical areas (B)</p> Signup and view all the answers

Which of the following conidia measurements is typical for Phialophora?

<p>3-5 by 1.5-3.5 micrometers (B)</p> Signup and view all the answers

Which characteristic is NOT associated with Rhinocladiella aquaspersa?

<p>Found exclusively in soil (A)</p> Signup and view all the answers

What type of infection is primarily caused by fungi in those with outdoor occupations?

<p>Subcutaneous and cutaneous infections (B)</p> Signup and view all the answers

Study Notes

Exophiala

  • Exophiala jeanselmei is a slow growing fungus that produces shiny brown, velvety colonies with age
  • It has elongated conidiophores with tapered tips, utilizes potassium nitrate, and grows only at 37°C

Exophiala dermatitidis

  • Slow grower
  • Produces shiny black colonies that appear velvety with age
  • Has elongated tubular annellophores
  • Does not use potassium nitrate
  • Grows at 37°C, 40°C, and 42°C

Conidia of Exophiala spp.

  • Conidia bud at the tips of annelides

Conidia of Exophiala dermatitidis

  • Yeast form: 2 to 4 μm in diameter, oval or elliptical in shape, often with a single bud
  • Cigar-shaped forms measuring 3 to 10 μm

Yeast & Mold Phases of S. dermatitidis

  • Colonies are gray-green to dark-brown

Dematiaceous Genera Inciting Subcutaneous Phaeohyphomycosis

  • Exserohilum: Gray-green to dark brown colonies
  • Alternaria: Golden-brown hyphae, large brown conidia resembling a drumstick, with horizontal and longitudinal septa

Cleistothecia Containing Ascospores

  • Appear as a drumstick with alternating dark and light horizontal and longitudinal septa

Curvularia

  • Geniculate conidiophores
  • Conidia: Golden-brown, multi-celled, curved, and have a swollen central cell

Bipolaris

  • Bent conidiophores
  • Oblong conidia arranged sympodially
  • Hilum protrudes slightly
  • Germ tubes present at one or both ends

Cladophialophora carrionii

  • Erect conidiophore
  • Single celled and branching

Rhinocladiella aquaspersa

  • Erect conidiophore
  • Dark in color, conidia located only on the upper portion near the tip of conidiophore
  • Elliptical, single-celled, and sympodially arranged

Phialophora vs. Cladophialophora

  • Phialophora: Conidia measure 3-5 by 1.5-3.5 micrometers, aggregated and clustered at the apices of the phialide
  • Cladophialophora: Lemoniform to fusiform, smooth, slightly verrucous, pale olivaceous

Phialophora verrucosa

  • Flask-shaped phialide
  • Collar-like structure (colorette)
  • Conidia

Eumycotic Mycetomas

  • Uncommon in the United States
  • Subcutaneous and cutaneous tissue infections
  • Swelling and exudates draining to the skin surface
  • Mostly found in tropical and subtropical areas
  • Infections occur in individuals with outdoor occupations
  • Chronic granulomatous infection, commonly involving the lower extremities
  • Dissemination may occur, but it is rare
  • Most common:
    • Pseudallescheria boydii (teleomorph): Sexual form, cleistotheicia
    • Scedosporium boydii(anamorph): Asexual form, single oval conidia

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Description

Explore the characteristics and growth conditions of the Exophiala genus, including Exophiala jeanselmei and Exophiala dermatitidis. This quiz covers their morphological features, conidia forms, and classification within dematiaceous fungi related to phaeohyphomycosis.

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