Exercise 13: Muscular Tissue

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Questions and Answers

Which of the following defines the primary function of the I band in a sarcomere?

  • Contains only thin filaments and bisected by the Z line. (correct)
  • Bisects the H band and consists of myosin filaments only.
  • Anchors actin filaments and bisects the I band.
  • Contains both actin and myosin filaments.

What structural feature distinguishes the H band within a sarcomere?

  • Darker coloration compared to the A band
  • Bisected by the Z line
  • Composed only of thick filaments (correct)
  • Presence of both actin and myosin filaments

How do nebulin and a-actinin contribute to the structural integrity of the sarcomere?

  • Nebulin stabilizes actin filaments, while a-actinin anchors them to the Z line. (correct)
  • Nebulin regulates myosin length, while a-actinin caps actin filaments
  • Both nebulin and a-actinin are responsible for cross-linking myosin filaments
  • Nebulin anchors myosin filaments, while a-actinin stabilizes actin filaments

In a resting muscle, what is the role of tropomyosin in relation to actin and myosin interaction?

<p>Tropomyosin covers the myosin-binding site on actin, preventing interaction. (C)</p>
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What is the significance of titin's location spanning from the Z line to the M line in a sarcomere?

<p>Titin helps center the thick filament and prevents overstretching of the sarcomere (B)</p>
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Which of the following accurately describe the characteristics of G-actin and F-actin?

<p>G-actin has myosin-binding site and forms the F-actin filament. (D)</p>
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Which of the following distinguishes the function of troponin T in muscle contraction?

<p>Binding to tropomyosin, which regulates actin accessibility. (A)</p>
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How do the structural features of skeletal muscle contribute to its specific function?

<p>Multinucleation supports high protein production for contraction. (B)</p>
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What is the primary role of calcium ions in skeletal muscle contraction?

<p>To bind to troponin, causing a shift in tropomyosin and exposing the myosin-binding sites on actin. (A)</p>
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Where would you most likely find skeletal muscle tissue?

<p>Attached to bones for movement (C)</p>
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How does the arrangement of endomysium, perimysium, and epimysium support the function of skeletal muscle?

<p>It provides structural support, pathways for blood vessels/nerves, and force transmission. (A)</p>
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Which of the following features are characteristic of skeletal muscle tissue when observed under a light microscope?

<p>Elongated, cylindrical cells with peripheral nuclei and cross-striations (A)</p>
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Although skeletal muscle is primarily attached to bones, where else might you find skeletal muscle performing a specialized function?

<p>The tongue (C)</p>
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Which of the following accurately describes the arrangement of muscle layers in the upper part of the esophagus?

<p>It contains skeletal muscle in the upper region, transitioning to smooth muscle (A)</p>
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Unlike skeletal muscle, cardiac muscle exhibits autorhythmicity. What cellular feature supports this function?

<p>Specialized pacemaker cells (A)</p>
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What is the functional significance of intercalated discs in cardiac muscle tissue?

<p>They electrically couple cells, allowing coordinated contraction. (A)</p>
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Cardiac muscle cells contain more mitochondria than skeletal muscle cells. How does this adaptation support cardiac muscle function?

<p>Increase ATP production for continuous activity. (C)</p>
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Where is cardiac muscle primarily located?

<p>In the walls of the heart (myocardium) (C)</p>
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What histological feature would help you distinguish cardiac muscle under a microscope?

<p>Branching cells with centrally located nuclei and intercalated discs (D)</p>
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In addition to the walls of the heart, where else can cardiac muscle be located?

<p>The walls of the large vessels attached to the heart (C)</p>
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Unlike skeletal and cardiac muscle, smooth muscle lacks sarcomeres. How does this difference affect its microscopic appearance?

<p>Smooth muscle appears uniform and non-striated. (B)</p>
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Calcium initiates contraction differently in smooth muscle compared to skeletal muscle. What replaces the troponin complex in smooth muscle cells?

<p>Calmodulin (B)</p>
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Smooth muscle is present in various body systems. Which of the following is a prime location for smooth muscle tissue?

<p>Walls of blood vessels and airways (D)</p>
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How does the lack of T-tubules in smooth muscle cells influence their mechanism of contraction and calcium regulation?

<p>Calcium ions primarily enter the cell from the sarcoplasmic reticulum and extracellular space through caveolae. (B)</p>
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Compared to skeletal muscle, what type of innervation primarily controls smooth muscle contraction?

<p>Autonomic nervous system (B)</p>
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Within the digestive system, smooth muscle is essential for peristalsis. Which structural feature of hollow organs facilitates this function?

<p>Layers of muscle tissue in the muscularis externa (B)</p>
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In smooth muscle, what is the function of dense bodies?

<p>Attachment points for actin filaments (A)</p>
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Which of the following describes the shape and nuclear characteristics of smooth muscle cells?

<p>Fusiform (spindle-shaped) cells with a single, central nucleus (A)</p>
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Where might you find smooth muscle contributing to regulation and control?

<p>Regulates pressure and airflow in the blood vessels and airways (A)</p>
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Unlike skeletal muscle fibers, smooth muscle cells are capable of both hypertrophy and hyperplasia. How does this affect tissue repair?

<p>Smooth muscle exhibits very different mitotic activity than most muscle cell. (A)</p>
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Which event directly triggers the cross-bridge cycle in smooth muscle contraction?

<p>Phosphorylation of myosin light chain (MLC) (C)</p>
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How does the structural organization of contractile filaments differ between skeletal and smooth muscle?

<p>Skeletal muscle's filaments are arranged into sarcomeres smooth muscle has a crisscross meshwork anchored to dense bodies (D)</p>
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Where do calcium ions primarily come from for contraction in smooth muscle?

<p>Both sarcoplasmic reticulum and outside the cell (C)</p>
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Which statement describes how smooth muscle contraction is modulated compared to skeletal muscle?

<p>Smooth muscle contractions are involuntary skeletal muscle uses nerves for contraction. (B)</p>
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What are the key distinctions between skeletal and cardiac muscle regarding control, structure, and location?

<p>Involuntary cardiac muscle contraction, voluntary skeletal muscle contraction, location. (C)</p>
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Smooth muscle contraction is regulated by the autonomic nervous system and various chemical signals. How does this complex regulation relate to its function?

<p>It allows for fine-tuned, adaptable control (C)</p>
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The sinoatrial (SA) node initiates the heart's rhythmic contractions without external nerve stimulation. How does that innate control relate to the structure of SA node cells?

<p>SA cells spontaneously depolarize and fire signals which can travel to other cells. (A)</p>
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Skeletal muscle is primarily responsible for voluntary movements. If a patient has damaged muscles, what activities might they have trouble with?

<p>Moving appendages. (B)</p>
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What is the functional significance of the arrangement of actin filaments being anchored to the Z line within a sarcomere?

<p>Establishing the boundaries of a sarcomere and transmitting force (D)</p>
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How does the absence of the troponin complex in smooth muscle affect its contraction mechanism compared to skeletal muscle?

<p>It depends on calmodulin binding to calcium ions to initiate contraction. (B)</p>
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How does the arrangement of smooth muscle layers in the digestive tract contribute to peristalsis?

<p>The circular layer constricts the organ, while the longitudinal layer shortens it. (C)</p>
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Epinephrine can trigger smooth muscle relaxation in the airways. How does this process occur?

<p>By inhibiting the activity of myosin light chain kinase (MLCK) (D)</p>
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How does the presence of numerous mitochondria in cardiac muscle cells support their continuous contractile activity?

<p>By ensuring a constant supply of ATP for cross-bridge cycling (B)</p>
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How do intercalated discs facilitate coordinated contraction in cardiac muscle?

<p>By enabling rapid ion flow and electrical impulse propagation (D)</p>
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Which mechanism explains how cardiac muscle function is affected if T-tubules are larger compared to skeletal muscle?

<p>Quicker conduction of action potentials into the cell interior (D)</p>
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What mechanism primarily contributes to the autorhythmicity of cardiac muscle?

<p>Pacemaker cells with unstable resting membrane potentials (D)</p>
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Within skeletal muscle, what is the functional consequence of having nuclei located peripherally within the muscle fiber?

<p>Optimizing space for myofibrils and contractile proteins (B)</p>
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How does the abundance of sarcoplasmic reticulum (SR) in skeletal muscle cells relate to their function?

<p>By storing and releasing calcium ions to initiate contraction (C)</p>
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Consider a muscle biopsy showing fibers with centrally located nuclei. What condition is most likely indicated by this observation?

<p>Muscle regeneration after injury (A)</p>
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Which adaptation would you expect to see in skeletal muscle fibers of a marathon runner compared to a sprinter?

<p>Greater proportion of slow-twitch muscle fibers (B)</p>
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How do the structural properties of dense bodies in smooth muscle contribute to its contractile function?

<p>Dense bodies anchor actin filaments, enabling force transmission during contraction. (C)</p>
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Which aspect of smooth muscle structure allows certain organs to maintain prolonged contractions without fatigue?

<p>The latch bridge mechanism (D)</p>
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How do the structural and functional characteristics and location of skeletal muscle support functions such as facial expressions?

<p>Elongated, cylindrical cells with multiple peripheral nuclei provide the structural basis and voluntary control required for varied expressions. (C)</p>
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What is the purpose of titin's elasticity within the sarcomere?

<p>To provide structural support and prevent over-stretching (A)</p>
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How does a muscle injury influence the regenerative capacity of skeletal muscle tissue?

<p>Satellite cells are activated to differentiate and fuse, aiding in muscle fiber repair. (C)</p>
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The upper part of the esophagus contains skeletal muscle. How does this arrangement relate to its function?

<p>It enables voluntary control over swallowing. (B)</p>
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How would you describe the arrangement of the myofibrils relative to one another within a single skeletal muscle cell?

<p>Tightly packed and parallel, extending the length of the cell (A)</p>
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Why does skeletal muscle need a rich blood supply?

<p>To deliver oxygen and nutrients for ATP production (C)</p>
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Cardiac muscle relies on extracellular calcium ions and calcium ions released from the sarcoplasmic reticulum. How does that dual control relate to its function?

<p>It leads to stronger and more sustained contractions. (B)</p>
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What is the main function of the connective tissue in muscles?

<p>Attachment and force transmission (C)</p>
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How does the regulation of calcium involving calmodulin rather than troponin complex affect smooth muscle contraction?

<p>Involuntary responses (A)</p>
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Although skeletal muscle is primarily responsible for movement, it supports joint stabilization. How would a patient with damaged muscles in the knee be affected?

<p>Impaired regulation in joint stability (D)</p>
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What happens when calcium binds to troponin in skeletal muscle?

<p>Myosin and actin binding (D)</p>
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How are cardiac muscle cells connected?

<p>Intercalated disks (D)</p>
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Where is smooth muscle found?

<p>Lining blood vessels (A)</p>
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What do dense bodies, caveolae, and gap junctions in SMOOTH MUSCLE do?

<p>Force transmission, structural adaptations, intercellular communication (C)</p>
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In a resting muscle, how do tropomyosin and troponin interact to regulate muscle contraction?

<p>Tropomyosin blocks the active site on actin and prevents myosin form binding (B)</p>
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Which muscles function and allows individuals to perform facial expressions?

<p>Skeletal muscle (B)</p>
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Which one of the statements describes the esophagus muscle?

<p>The upper third of the esophagus consists of skeletal muscle. (C)</p>
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Smooth muscle is in the blood vessels because...

<p>Regulate blood pressure (D)</p>
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Main role of connective tissue in muscle?

<p>Support attachment and force transmission (D)</p>
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What are the functional outcomes for damaged skeletal muscle?

<p>Impair the joint stabilization and functionality. (C)</p>
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Which outcome is most likely observed in smooth muscle compared to skeletal muscle?

<p>Smooth muscle allows cells to undergo hypertrophy and hyperplasia, while skeletal muscle primarily undergoes hypertrophy. (A)</p>
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Cardiac muscle rely on

<p>Calcium ions from the sarcoplasmic reticulum and outside the cell. (C)</p>
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If somebody has cardiac muscle function problems, what activities would be affected?

<p>Heart contraction (D)</p>
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A sarcomere contains different zones such as the I band. How would you describe the I band?

<p>Contains ONLY thin filaments (B)</p>
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How does the arrangement of actin and myosin filaments contribute to the characteristic striated pattern observed in skeletal muscle?

<p>The precise organization of actin and myosin into sarcomeres produces repeating units of alternating density. (A)</p>
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What is the functional consequence of the larger T-tubules and abundant mitochondria observed in cardiac muscle compared to skeletal muscle?

<p>Enhanced oxygen delivery and sustained contractile activity. (B)</p>
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A pathologist observes a muscle biopsy with centrally located nuclei and branching fibers. Which muscle type is most likely represented in the sample?

<p>Cardiac muscle due to the combination of central nuclei and branching fibers. (B)</p>
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How does the autonomic nervous system influence smooth muscle contraction differently than its effect on skeletal muscle?

<p>It modulates contraction through chemical signals and involuntary control, contrasting with the voluntary control of skeletal muscle. (A)</p>
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During a muscle contraction, what is the collective role of accessory proteins such as nebulin, titin, and a-actinin within a sarcomere?

<p>To regulate the spacing, attachment, and alignment of myofilaments within the sarcomere to ensure structural integrity. (D)</p>
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Flashcards

Sarcomere

The region of a myofibril between two successive Z discs; the functional unit of muscle contraction.

A band

The entire length of the thick filaments, containing both actin and myosin filaments, appearing as a darker colored area under a microscope.

H band

Thinner band bisecting the A band, appearing as a lighter colored area under a microscope.

M line

The line that bisects the H band and appears as a darker colored line under a microscope.

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I band

Contains thin filaments, bisected by the Z line, and appears as a lighter colored area.

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Z line

Anchors actin filaments; bisects the I band; appears as a darker colored line.

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G-actin

Attaches to the Z line and extends to the A band, forming the F-actin filament and possessing a myosin-binding site.

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F-actin

A double-stranded helix with a plus end bound to the Z line, whose minus end extends towards the M line.

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Tropomyosin

A double helix of two polypeptides that runs in the groove between the F-actin molecules, covering myosin-binding sites in resting muscle.

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Troponin Complex

A complex that binds calcium (C), tropomyosin (T), and actin (I) in muscle.

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Tropomodulin

Attached to the minus end of the thin filament, it maintains and regulates the length of the actin filament.

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Nebulin

Elongated and inelastic protein attached to the Z lines, adding stability to thin filaments anchored by a-actinin in Z lines.

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Myosin

Rod shaped, motor protein composed of two heavy polypeptide chains and four light chains.

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Titin

From the Z line to the M line, helps center the thick filament in the middle between two Z lines, and prevents excessive stretching.

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Alpha-Actinin

An actin-binding protein that anchors thin filaments at the Z line and cross-links titin embedded in the Z line.

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Desmin

Connects the Z-lines of neighboring sarcomeres and attaches the Z lines to the sarcolemma via ankyrin.

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M Line Proteins

Proteins that attach titin to the thick filament and hold thick filaments at the M line.

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Dystrophin

Links the external lamina of muscle cell to actin filaments.

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Skeletal Muscle

Elongated, cylindrical cells with multinucleated, peripherally located nuclei, displaying cross striations.

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Skeletal Muscle Locations

Muscles attached to bones, skin (facial muscles), tongue, and the upper part of the Esophagus.

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Functions of Skeletal Muscle

Includes production of bodily movement, maintaining posture, stabilizing joints, and producing heat.

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Cardiac Muscle

Cells are shorter, show branching, have one centrally located nucleus, and are connected by intercalated discs, exhibiting autorhythmicity.

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Location of Cardiac Muscle

myocardium and walls of large vessels

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Cardiac muscle functions

Contraction, Pacemaker, Impulse propagation

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Smooth Muscle

Cells are fusiform, possess a single nucleus, lack striations; calcium ions from the sarcoplasmic reticulum and outside the cell; calmodulin instead of troponin.

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Location of Smooth Muscle

Integumentary system, blood vessels & airways, reproductive & glandular, digestive & urinary system.

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Smooth muscle functions.

Typical functions of Smooth muscle

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Study Notes

  • MT120225 Exercise 13 focuses on muscular tissue
  • The topic outline will cover sarcomere, skeletal muscle, cardiac muscle, smooth muscle, and laboratory activities
  • Upon completion of the exercise, students should be able to identify protein components and correlate myofibril components with their structural or functional properties

Sarcomere and Bands/Lines

  • The A band spans the entire length of the thick filaments and contains both actin and myosin filaments; it appears darker in color
  • The H band is a thinner band bisecting the A band and has a lighter colored area
  • The M line bisects the H band and looks darker in color
  • The I band contains thin filaments and is bisected by the Z line; it looks lighter colored
  • The Z line serves as an anchor for actin filaments and bisects the I band; it looks darker in color

Thin Filaments

  • Attach to the Z line and extend into the A band to the edge of the H band.
  • G-actin forms the F-actin filament and has a myosin-binding site
  • F-actin is a double-stranded helix.
  • The plus end of F-actin is bound to the Z line by alpha-actinin with nebulin
  • The minus end of F-actin extends toward the M line

Thin Filaments cont...

  • Tropomyosin is a double helix made of two polypeptides which runs in the groove between F-actin molecules
  • In resting muscle, tropomyosin covers the myosin binding site on the actin molecule
  • Troponin complex has three components
  • Troponin C binds calcium
  • Troponin T binds to tropomyosin
  • Troponin I binds to actin
  • Tropomodulin is attached to the minus end of the thin filament and maintains and regulates the length of the actin filament
  • Nebulin is an elongated and inelastic protein attached to the Z lines stabilizing the thin filaments.

Thick Filaments

  • Myosin is a rod-shaped motor protein that is composed of two heavy polypeptide chains and four light chains
  • It has two globular heads connected via lever arms with a long tail
  • Myosin exists as a monomer with one ELC and one RLC
  • The RLC stabilizes the lever arm
  • The head of Myosin has binding sites for ATP and actin
  • M line connects thick filaments

Accessory Proteins

  • Accessory proteins regulate the spacing, attachment, and alignment of the myofilaments
  • Titin runs from the Z line to the M line, helps center the thick filament between two Z lines, and prevents excessive stretching
  • Alpha-actinin-binding protein anchors thin filaments at or in the Z line and cross-links titin embedded in the Z line
  • Desmin connects the Z-lines of neighboring sarcomeres and attaches the Z lines to the sarcolemma via ankyrin
  • M line proteins attach titin to the thick filament and holds the thick filaments at the M line
  • Dystrophin links the external lamina of the muscle cell to actin filaments.

Skeletal Muscle

  • Characterized by elongated, cylindrical, multinucleated cells with nuclei on the periphery and has cross striations in longitudinal section
  • Calcium ions are from sarcoplasmic reticulum
  • Location includes muscles attached to bones, to the skin (facial muscles), in the tongue, and the upper esophagus
  • Functions include bodily movement, maintaining posture, stabilization of joints, and heat production

Cardiac Muscle

  • Characterized by shorter cells that show branching with one centrally located nucleus and show striations in longitudinal section
  • Cells are connected to each other by intercalated discs and have larger T-tubules and more abundant mitochondria
  • Sarcoplasmic reticulum is less developed
  • Sarcoplasm contains fatty droplets, glycogen particles, and lipofuscin granules
  • Calcium ions originate from the sarcoplasmic reticulum and outside the cell
  • Exhibits autorhythmicity and spontaneously generates stimuli

Cardiac Muscle Locations and Functions

  • Location occurs in the walls of the heart, specifically the myocardium
  • Cardiac muscle exists in the walls of the large vessels attached to the heart (aorta & pulmonary trunk)
  • Functions to contract
  • Cardiac muscle serves as a pacemaker and for impulse propagation
  • The layers of the heart wall are the simple squamous epithelium, the loose connective tissue, the epicardium, the myocardium and the endocardium

Purkinje Fibers

  • Modified cardiac muscle fibers, found near the endocardium of the heart

Smooth Muscle

  • Characterized by fusiform cells that are elongated and tapering at the ends, closely packed with a single centrally located nucleus, no t-tubules but has caveolae
  • There are no striations and no sarcomeres
  • Thin and thick filaments crisscross obliquely forming a network in the cell
  • Dense bodies appear in sarcoplasm and sarcolemma
  • Cells produce components of extracellular matrix
  • Calcium ions are from sarcoplasmic reticulum and outside the cell
  • Troponin complex is replaced by calmodulin

Location and Function of Smooth Muscle

  • Smooth muscle is located in the integumentary system causing arrector pili muscles that make "goose bumps"
  • Smooth muscle exists in blood vessels and airways, regulating pressure and airflow
  • Smooth muscle lives in reproductive and glandular systems to produce movements
  • Smooth muscle is in the digestive and urinary systems to form sphincters and produce contractions
  • Functions for contraction and the synthesis of proteoglycan, elastin, and precursors of collagen fibers

Structure of Hollow Organs

  • The typical structure of hollow organs is from the outer surface going inwards
  • Serosa
  • Subserosa
  • Muscularis-Thick muscle layers
  • Submucosa
  • Thin muscle layer
  • Connective Tissue
  • Epithelium

Sarcomere Diagram: Summary

  • A - A band
  • B - Troponin
  • C - α-actinin
  • D - Titin
  • E - Myosin
  • F - M Line
  • G - H Band
  • H - I Band
  1. Titin is a large elastic protein that forms a lattice and serve to anchor the thick filaments to the Z disk
  2. Myosin is organized into aggregates tail-to-tail to form the thick filaments of striated muscles
  3. The M line is the region in the sarcomere that bisects the isotropic band
  4. The A band contributes to the striated appearance of skeletal and cardiac muscle under a polarizing microscope
  5. The H band is the area within the A band that is devoid of actin
  6. α-actinin is the attachment site for the thick filaments
  7. The I Band is the area found on either side of the Z disc that contains the thin filaments and titin
  8. α-actinin is the main contractile protein of the thin filament

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