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Questions and Answers

What is the primary function of the plasma membrane?

  • To facilitate chemical reactions
  • To control traffic into and out of the cell (correct)
  • To store genetic information
  • To produce energy for the cell

Which statement best describes the Fluid Mosaic Model?

  • The membrane consists of a uniform layer of phospholipids only.
  • The membrane is static and does not allow for movement.
  • The membrane is a mosaic of proteins embedded in a fluid bilayer. (correct)
  • The membrane is a rigid structure with fixed proteins.

What are phospholipids primarily known for in the plasma membrane?

  • Serving as receptors for signaling molecules
  • Being the most abundant lipid in the membrane (correct)
  • Facilitating active transport across the membrane
  • Providing structural stability

Which of the following mechanisms does NOT contribute to selective permeability of the plasma membrane?

<p>High temperatures stabilizing the membrane (C)</p> Signup and view all the answers

What is a characteristic feature of membrane proteins?

<p>They serve specific functions such as transport and signaling. (B)</p> Signup and view all the answers

How do hydrophobic interactions contribute to plasma membrane structure?

<p>They hold the phospholipid bilayer together. (D)</p> Signup and view all the answers

Which component of the plasma membrane is responsible for selective transport of molecules?

<p>Membrane proteins (C)</p> Signup and view all the answers

What effect do temperature changes have on membrane fluidity?

<p>Increased temperature can enhance membrane fluidity. (C)</p> Signup and view all the answers

What role do membrane carbohydrates play in cell recognition?

<p>They assist in cell-cell recognition. (A)</p> Signup and view all the answers

Which of the following best describes glycoproteins?

<p>Proteins that have carbohydrates covalently bonded to them. (D)</p> Signup and view all the answers

What mechanism is involved in the rejection of foreign cells by the immune system?

<p>Cell-cell recognition. (C)</p> Signup and view all the answers

Which statement is true about gap junctions?

<p>They allow the passage of molecules directly between adjacent cells. (D)</p> Signup and view all the answers

What property of the cell membrane primarily limits the passage of many molecules?

<p>The hydrophobic core of the lipid bilayer. (B)</p> Signup and view all the answers

How do large uncharged polar molecules generally cross the cell membrane?

<p>They require specific transport proteins. (B)</p> Signup and view all the answers

What is the function of microfilaments in relation to membrane proteins?

<p>They stabilize the location of certain membrane proteins. (D)</p> Signup and view all the answers

What happens to a cell placed in a hypotonic solution?

<p>Water enters the cell and it may burst. (C)</p> Signup and view all the answers

Which of these is NOT a characteristic of cell-cell recognition?

<p>It prevents the merging of similar cells. (D)</p> Signup and view all the answers

In which type of solution does a cell maintain its volume without net movement of water?

<p>Isotonic solution (C)</p> Signup and view all the answers

What is the primary effect of placing a plant cell in a hypertonic solution?

<p>The plasma membrane pulls away from the cell wall. (A)</p> Signup and view all the answers

What is the definition of tonicity?

<p>The ability of a solution to cause a cell to gain or lose water. (A)</p> Signup and view all the answers

What occurs when an animal cell is placed in an isotonic environment?

<p>There is no net movement of water. (D)</p> Signup and view all the answers

What primarily happens to an animal cell in a hypertonic solution?

<p>The cell may shrivel and die. (A)</p> Signup and view all the answers

How does a plant cell respond to a hypotonic environment?

<p>The cell becomes turgid and firm. (C)</p> Signup and view all the answers

What is the result of increasing salinity in a lake for animal cells?

<p>Animal cells lose water to their environment. (A)</p> Signup and view all the answers

What is the main function of transport proteins in facilitated diffusion?

<p>To help solutes cross membranes while following their concentration gradient (C)</p> Signup and view all the answers

Which type of transport protein provides specific passageways for molecules and ions?

<p>Channel protein (C)</p> Signup and view all the answers

How do aquaporins function in plant and animal cells?

<p>By facilitating rapid diffusion of water molecules across the membrane (C)</p> Signup and view all the answers

What distinguishes gated ion channels from non-gated ion channels?

<p>Gated channels open or close in response to stimuli, non-gated do not (C)</p> Signup and view all the answers

Which stimulus can cause a gated ion channel to open or close?

<p>Chemical ligand binding (B)</p> Signup and view all the answers

Which characteristic is true of non-gated or leak channels?

<p>They are always open and facilitate ion permeability (C)</p> Signup and view all the answers

What process is plasmolysis associated with in plant cells?

<p>Cell contraction and wilting (A)</p> Signup and view all the answers

What is the primary role of carrier proteins in facilitated diffusion?

<p>To bind specific molecules and transport them across the membrane (B)</p> Signup and view all the answers

What triggers the opening of mechanically-gated ion channels?

<p>Physical deformation (C)</p> Signup and view all the answers

What is the primary defect in the chloride channels of individuals with cystic fibrosis?

<p>Defective or absent chloride transport (B)</p> Signup and view all the answers

What effect does the high concentration of intracellular Cl- have in cystic fibrosis?

<p>Inhibits mucus clearance by cilia (C)</p> Signup and view all the answers

Which of the following accurately describes the mechanism of carrier proteins?

<p>They bind solutes and undergo a shape change. (C)</p> Signup and view all the answers

What is the primary consequence of defective carrier proteins in cystinuria?

<p>Accumulation of cystine in urine (B)</p> Signup and view all the answers

What common complication arises from the accumulation of thick mucus in cystic fibrosis patients?

<p>Respiratory infections (A)</p> Signup and view all the answers

How does extracellular Cl- contribute to mucus quality in cystic fibrosis?

<p>It attracts water to maintain mucus consistency. (B)</p> Signup and view all the answers

What is a common symptom of cystinuria due to the accumulation of cystine?

<p>Formation of kidney stones (B)</p> Signup and view all the answers

What is the main purpose of active transport in cells?

<p>To enable cells to maintain specific internal concentrations of solutes. (D)</p> Signup and view all the answers

Which of the following accurately describes the sodium-potassium pump?

<p>It exchanges Na+ for K+ against their concentration gradients. (A)</p> Signup and view all the answers

What is generated across a membrane by an electrogenic pump?

<p>An electrochemical gradient. (A)</p> Signup and view all the answers

Which component is crucial for the functioning of cotransport systems?

<p>The movement of one solute down its concentration gradient. (C)</p> Signup and view all the answers

What role does ATP play in active transport?

<p>It directly powers the transport of solutes across the membrane. (D)</p> Signup and view all the answers

What happens when the proton pump operates in plant cells?

<p>H+ ions accumulate outside the cell. (C)</p> Signup and view all the answers

What is a key benefit of having a high concentration of ions outside the cell in the context of cotransport?

<p>It drives the uptake of nutrients against their gradients. (D)</p> Signup and view all the answers

Which of the following is an example of an electrogenic pump?

<p>The sodium-potassium pump. (A)</p> Signup and view all the answers

Flashcards

Plasma membrane function

Separates the cell from its surroundings, controlling traffic of substances in and out, and exhibiting selective permeability.

Phospholipids

The most abundant lipid in the plasma membrane, an amphipathic molecule with both hydrophilic and hydrophobic regions.

Fluid Mosaic Model

A model describing the plasma membrane as a mosaic of proteins embedded in a fluid phospholipid bilayer.

Selective permeability

The ability of the plasma membrane to regulate what enters and leaves the cell.

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Membrane fluidity

The ability of the membrane to change shape and allow movement of components.

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Hydrophobic region

The part of a molecule that repels water.

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Hydrophilic region

The part of a molecule that attracts water.

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Membrane proteins role

Embedded into the lipid bilayer of the plasma membrane, proteins play a crucial role in regulating the passage of molecules, cell recognition, and cell signaling.

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Cell-cell recognition

A cell's ability to distinguish one type of neighboring cell from another.

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Membrane carbohydrates

Carbohydrates attached to lipids (glycolipids) or proteins (glycoproteins) on the cell membrane.

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Cell recognition molecules

Surface molecules (often carbs or proteins) that allow cells to recognize each other.

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Intercellular joining molecules

Membrane proteins that connect adjacent cells through junctions.

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Gap junctions

Intercellular connections that allow cytoplasmic channels between cells.

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Tight junctions

Intercellular connections that prevent leakage of extracellular fluid.

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Cytoskeleton attachment

Membrane proteins that connect to the cell's internal framework (cytoskeleton).

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Plasmolysis

The process in which plant cells lose water and shrink due to a hypertonic environment, resulting in wilting.

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Passive Transport

Movement of substances across a membrane from an area of high concentration to an area of low concentration, without energy expenditure.

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Facilitated Diffusion

A type of passive transport where molecules move across a membrane with the help of transport proteins.

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Transport Proteins

Membrane proteins that facilitate the movement of specific molecules across the cell membrane.

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Channel Protein

A type of transport protein that acts as a tunnel, allowing specific molecules or ions to pass through the membrane.

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Aquaporins

Water channel proteins that facilitate the rapid movement of water molecules across cell membranes.

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Ion Channel

A transmembrane protein channel that allows specific ions to pass through the membrane.

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Gated Ion Channels

Ion channels that can open or close in response to a specific stimulus like a chemical, electrical, or mechanical change.

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Tonicity

The ability of a solution surrounding a cell to cause that cell to gain or lose water.

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Isotonic solution

A solution with the same solute concentration as the inside of a cell. No net water movement occurs.

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Hypertonic solution

A solution with a higher solute concentration than the inside of a cell. Water leaves the cell.

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Hypotonic solution

A solution with a lower solute concentration than the inside of a cell. Water enters the cell.

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What happens to an animal cell in a hypotonic solution?

The cell will swell and lyse (burst) because water moves into the cell faster than it leaves.

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What happens to an animal cell in a hypertonic solution?

The cell will lose water to its environment, shrink, and potentially die.

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What happens to a plant cell in a hypotonic solution?

The cell swells but does not burst. It becomes turgid (firm), due to the cell wall.

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What happens to a plant cell in a hypertonic solution?

The cell loses water, the vacuole shrinks, the plasma membrane pulls away from the cell wall, and the cell becomes flaccid (wilts).

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Mechanically-gated channels

These channels open in response to physical deformation of the receptor, like pressure or touch. They're found in sensory receptors of the skin and ear.

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Cystic Fibrosis: Defective protein

Cystic fibrosis is caused by a mutated gene that leads to defective or missing chloride channel proteins. These proteins are essential for the proper movement of water and salt across cell membranes.

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Cystic Fibrosis: Mucus buildup

The defective chloride channels in cystic fibrosis result in thicker and stickier mucus in the lungs, pancreas, and other organs. This mucus buildup can lead to infections and blockages.

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Cystinuria: Defective carrier protein

Cystinuria is caused by defects in the carrier proteins that transport cystine (an amino acid) across the membranes of kidney cells. This leads to cystine buildup in the urine, forming crystals and stones.

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Facilitated diffusion: Carrier proteins

Facilitated diffusion is a form of passive transport where carrier proteins help small hydrophilic molecules, like glucose and amino acids, cross the cell membrane. This process doesn't require energy.

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Carrier protein function

Carrier proteins change shape when a solute binds to their binding site, allowing the transported molecule to be released across the membrane. This process doesn't require energy.

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Glucose transport: Carrier protein

The glucose transporter is a specific carrier protein that binds to glucose, changes shape, and releases glucose into the cell. This process is an example of facilitated diffusion.

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Cystinuria: Cystine buildup

In cystinuria, the defective carrier proteins in the kidneys prevent cystine from being reabsorbed into the bloodstream. This leads to an increase in cystine in the urine, which can form crystals and stones.

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Electrogenic Pump

A transport protein that generates voltage across a membrane by creating an uneven distribution of ions.

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Sodium-Potassium Pump

An electrogenic pump found in animal cells that pumps 3 Na+ ions out of the cell for every 2 K+ ions pumped in, creating a concentration gradient and a membrane potential.

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Proton Pump

An electrogenic pump found in plants, fungi, and bacteria that pumps protons (H+) out of the cell, creating a proton gradient and a membrane potential.

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Cotransport

A system where the movement of one solute down its concentration gradient provides energy for the transport of another solute against its gradient.

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H+ - sucrose cotransporter

A type of cotransport in plants where the movement of protons (H+) down its concentration gradient provides energy for the uptake of sucrose against its gradient.

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What does active transport require?

Active transport requires energy expenditure, which is provided by ATP. It also relies on carrier proteins to move molecules against their concentration gradient.

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Why do cells need active transport?

Active transport allows cells to maintain an internal concentration of small solutes that differ from the environment, enabling them to function optimally.

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Study Notes

Plasma Membrane Transport

  • Chapter 3 of BIO091, Semester 1, 2024/2025
  • Learning outcomes:
    • List components of plasma membrane and their function
    • Describe plasma membrane structure and the functions of components
    • Explain the role of membrane proteins
    • Explain how membrane structure results in selective permeability
    • Explain various transport mechanisms across the membrane

Membrane Components and Organization

  • The plasma membrane separates the living cell from its surroundings.
  • It controls traffic into and out of the cell
  • It exhibits selective permeability.
  • Phospholipids: The most abundant lipid in the plasma membrane, amphipathic (hydrophobic and hydrophilic regions).

Structure of Plasma Membrane - Fluid Mosaic Model

  • Proposed by Singer and Nicolson in 1972.
  • The membrane is a mosaic of proteins embedded in a fluid bilayer of phospholipids.

Membrane Proteins and Their Functions

  • Peripheral proteins: Not embedded in the lipid bilayer, located on the inner or outer surface, usually bound to exposed regions of integral proteins through noncovalent interactions.
  • Integral proteins: Penetrate the hydrophobic core; amphipathic proteins.
    • Hydrophilic segments are non-helical in contact with intracellular and extracellular fluids.
    • Hydrophobic segments are a-helical secondary structures or rolled-up B-pleated sheets).
  • Six major functions of membrane proteins:
    • Transport proteins
    • Involved in enzymatic activity
    • Involved in signal transduction processes
    • Involved in cell-cell recognition
    • As intercellular joining molecules
    • Attach to the cytoskeleton and extracellular matrix (ECM)

Membrane Structure Results in Selective Permeability

  • Small hydrophobic molecules: Can dissolve in the lipid bilayer and pass through it. (e.g., hydrocarbons, gases like CO2 and O2, and small polar but uncharged molecules like H2O and ethanol).
  • Hydrophilic molecules: Cannot easily pass through the hydrophobic core of the membrane, and often require transport proteins. (e.g., large uncharged polar molecules like glucose, small ions such as H+, Na+ and K+).
  • Transport Proteins: Allow movement of hydrophilic molecules or ions across the membrane.
    • Channel proteins: Hydrophilic channels that specific molecules or ions use as tunnels.
      • Aquaporins: Specific channel proteins that facilitate the passage of water.
      • Ion channels: Non-gated (leak): Always open and responsible for permeability of specific types of ions.
        • Gated: Open or close in response to stimulus (chemical, electrical or mechanical).
    • Carrier proteins: Bind to molecules and change shape to pass them across the membrane, specific to the substance it moves.

Passive Transport

  • Types of passive transports:
    • Simple Diffusion
    • Osmosis
    • Facilitated Diffusion.
  • Substances move down their concentration gradient without energy input.

Active Transport

  • Movement of ions or molecules across the membrane against their concentration gradient (needs energy).
  • Uses carrier proteins, requiring ATP.
  • Types of active transport:
    • Electrogenic pump: Transport protein that generates a voltage across a membrane (e.g sodium-potassium pump, proton pump).
    • Cotransport: Two different solutes are transported simultaneously. One solute moves down its concentration gradient, providing the energy to move the other solute against its concentration gradient (e.g. H+-sucrose cotransporter, Na+-glucose cotransporter).
    • Bulk transport:
      • Endocytosis—movement of materials into the cell by forming vesicles. Examples include Phagocytosis, Pinocytosis, and receptor-mediated endocytosis.
      • Exocytosis—movement of materials out of the cell by fusion of vesicles with the plasma membrane.
  • Cystic Fibrosis: Genetic disease causing defective or absent chloride channels, resulting in mucus buildup in various organs.
  • Cystinuria: Inherited disease causing accumulation of cystine leading to crystals and stones blocking the kidneys, ureters, or bladder.
  • Hypercholesterolemia: Disease related to defective or missing LDL receptor proteins causing cholesterol accumulation in the blood, contributing to early atherosclerosis.

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