Eukaryotic Cell Biology: Cell Junctions
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Questions and Answers

Cells are bound together via ______ complexes.

junctional

Epithelial sheets connect cells to form structures that line ______ and surfaces.

cavities

The apical surface is free and exposed to the air or to a watery ______.

fluid

The basal surface of epithelial cells is attached to a sheet of connective tissue called the ______.

<p>basal lamina</p> Signup and view all the answers

In many tissues, each cell is separated from the next by an extracellular ______.

<p>matrix</p> Signup and view all the answers

There are five different types of cell ______ found in epithelial cells.

<p>junctions</p> Signup and view all the answers

Tight junctions act as barriers between fluid ______.

<p>compartments</p> Signup and view all the answers

Tight junctions form seals that prevent the free passage of molecules between the cells of ______ sheets.

<p>epithelial</p> Signup and view all the answers

One function of tight junctions is to separate the apical and ______ domains of the plasma membrane.

<p>basolateral</p> Signup and view all the answers

Occludin is a major component of the tight junction and is a ______ protein.

<p>transmembrane</p> Signup and view all the answers

Tight junctions prevent the free diffusion of lipids and membrane ______ between the apical and basolateral domains.

<p>proteins</p> Signup and view all the answers

Tight junctions allow ______ transport and paracellular transport.

<p>transcellular</p> Signup and view all the answers

The tight junction protein Occludin has a short N-terminal and a much longer ______ region exposed in the cytosol.

<p>C-terminal</p> Signup and view all the answers

The binding to occludin on adjacent cells is known as ______ binding.

<p>homophilic</p> Signup and view all the answers

The first extracellular loop of claudin is hypothesized to determine paracellular tightness and selective ion ______.

<p>permeability</p> Signup and view all the answers

Junction Adhesion Molecules (JAM) are part of the immunoglobulin ______ family.

<p>super</p> Signup and view all the answers

Tight junctions help to regulate the paracellular pathway function and maintain cell ______.

<p>polarity</p> Signup and view all the answers

Tight junctions provide minimal adhesive strength compared to ______ junctions.

<p>adherens</p> Signup and view all the answers

Cadherins, which are responsible for adherens junctions, require ______ to bind effectively.

<p>calcium</p> Signup and view all the answers

Tight junction dysfunction is characterized by a reduction of tight junction ______.

<p>strands</p> Signup and view all the answers

Anchoring junctions connect the cytoskeleton to a neighbor or to the ______.

<p>ECM</p> Signup and view all the answers

Linker proteins β-catenin and p120 bind to the cytosolic tails of cadherins binding it to ______.

<p>F-actin</p> Signup and view all the answers

E-cadherin acts as a tumour ______.

<p>suppressor</p> Signup and view all the answers

Desmosomes are similar to ______ junctions.

<p>adherens</p> Signup and view all the answers

Desmosomes contain specialised nonclassical cadherins that link to ______ filaments instead of actin filaments.

<p>intermediate</p> Signup and view all the answers

Pemphigus is a severe autoimmune blistering skin disease caused by autoantibodies primarily targeting the ______ required for firm adhesion.

<p>desmogleins</p> Signup and view all the answers

Hemidesmosomes anchor cells to the ______ lamina.

<p>basal</p> Signup and view all the answers

Integrins in hemidesmosomes attach to intermediate filaments and the protein ______ present in the basal lamina.

<p>laminin</p> Signup and view all the answers

Gap junctions provide direct connections between the ______ of adjacent cells.

<p>cytoplasm</p> Signup and view all the answers

Gap junctions are constructed of four-pass transmembrane proteins called ______.

<p>connexins</p> Signup and view all the answers

Each gap junction consists of many ______ pairs in parallel.

<p>connexon</p> Signup and view all the answers

Gap junctions allow ions and small molecules to diffuse between ______ cells.

<p>adjacent</p> Signup and view all the answers

There are at least ______ different human connexin proteins.

<p>21</p> Signup and view all the answers

Tight junctions help polarize cells and prevent leakage of extracellular ______.

<p>molecules</p> Signup and view all the answers

The main proteins found at adherens junctions are ______ molecules.

<p>cadherin</p> Signup and view all the answers

Gap junctions couple cells metabolically, allowing one cell to provide necessary ______ to neighboring cells.

<p>nutrients</p> Signup and view all the answers

Smooth muscle cells communicate through ______ junctions.

<p>gap</p> Signup and view all the answers

Epithelial cells connect to one another to form epithelial sheets that line ______ throughout the animal body.

<p>cavities</p> Signup and view all the answers

The apical surface of epithelial cells is exposed to the air or to a watery ______.

<p>fluid</p> Signup and view all the answers

Connective tissue is composed of cells surrounded by an extensive ______ matrix.

<p>extracellular</p> Signup and view all the answers

The basal surface of epithelial cells is attached to a sheet of connective tissue called the basal ______.

<p>lamina</p> Signup and view all the answers

Tight junctions act as barriers between fluid compartments and help to determine the ______ of the plasma membrane.

<p>polarity</p> Signup and view all the answers

Homophilic binding refers to binding between ______ cells.

<p>similar</p> Signup and view all the answers

Claudin proteins are hypothesized to determine the paracellular tightness and selective ion ______.

<p>permeability</p> Signup and view all the answers

Tight junction dysfunction is observed in conditions such as ______ disease.

<p>Crohn's</p> Signup and view all the answers

Cadherins are a family of proteins that require ______ to bind to one another.

<p>calcium</p> Signup and view all the answers

The intracellular tails of transmembrane proteins in tight junctions bind to cytosolic proteins in the zonula ______ family.

<p>occludens</p> Signup and view all the answers

Desmosomes contain specialized nonclassical cadherins that link to ______ filaments.

<p>intermediate</p> Signup and view all the answers

Transmembrane proteins called ______ are major components of tight junctions.

<p>Occludin</p> Signup and view all the answers

Tight junctions separate the ______ and basolateral domains of the plasma membrane.

<p>apical</p> Signup and view all the answers

Tight junctions form seals that prevent the free passage of ______ between cells.

<p>molecules</p> Signup and view all the answers

The main function of tight junctions is to act as barriers between fluid ______.

<p>compartments</p> Signup and view all the answers

One type of junction that connects the cytoskeleton to neighboring cells is called a ______ junction.

<p>desmosome</p> Signup and view all the answers

Hemidesmosomes anchor epithelial cells to the ______ lamina.

<p>basal</p> Signup and view all the answers

Cadherins, which are a key component of adherens junctions, require ______ to bind effectively.

<p>calcium</p> Signup and view all the answers

Gap junctions allow for direct communication between the ______ of adjacent cells.

<p>cytoplasm</p> Signup and view all the answers

Desmosomes provide mechanical ______ to epithelia.

<p>strength</p> Signup and view all the answers

The loss of E-cadherin correlates with increased invasiveness and ______ of tumors.

<p>metastasis</p> Signup and view all the answers

Desmosomes resist mechanical stress due to their strongly ______ state.

<p>adhesive</p> Signup and view all the answers

Gap junctions are formed from different combinations of ______, which create channels with varying properties.

<p>connexins</p> Signup and view all the answers

A connexon is a structure formed when ______ connexins assemble together.

<p>six</p> Signup and view all the answers

Gap junctions allow for the movement of ______ and small molecules between adjacent cells.

<p>ions</p> Signup and view all the answers

The main function of gap junctions is to couple cells ______ and electrically.

<p>metabolically</p> Signup and view all the answers

Gap junctions consist of connexon pairs that form a sort of ______ to facilitate communication.

<p>molecular sieve</p> Signup and view all the answers

Cardiac and smooth muscle communicate primarily through ______ junctions.

<p>gap</p> Signup and view all the answers

Different tissues can exhibit varying ______ and regulation in their gap junctions.

<p>permeability</p> Signup and view all the answers

In heart muscle, gap junctions ensure that electrical signals are ______ to neighbouring cells.

<p>transmitted</p> Signup and view all the answers

What is the primary purpose of tight junctions in epithelial cells?

<p>To prevent leakage between the apical and basolateral domains</p> Signup and view all the answers

Which of the following proteins are crucial for the adhesion in adherens junctions?

<p>Cadherins</p> Signup and view all the answers

What distinguishes epithelial sheets from connective tissues in terms of cellular arrangement?

<p>Epithelial cells are frequently polarized and tightly connected</p> Signup and view all the answers

Which type of junction connects the cytoskeleton to neighboring cells, providing mechanical strength?

<p>Desmosomes</p> Signup and view all the answers

What role do gap junctions play in cellular communication?

<p>They facilitate the exchange of ions and small molecules</p> Signup and view all the answers

Which transmembrane protein is a major component of tight junctions?

<p>Occludin</p> Signup and view all the answers

Which statement accurately describes the function of tight junctions?

<p>They prevent the diffusion of lipids and membrane proteins.</p> Signup and view all the answers

What characterizes the arrangement of proteins in tight junctions?

<p>They consist largely of transmembrane proteins.</p> Signup and view all the answers

What is the significance of the C-terminal region of Occludin?

<p>It is involved in anchoring the tight junction complex.</p> Signup and view all the answers

What is one method by which tight junctions manage transport across epithelial cells?

<p>Utilizing transcellular and paracellular transport pathways.</p> Signup and view all the answers

What type of junction is primarily involved in the adhesion process between epithelial cells?

<p>Desmosomes</p> Signup and view all the answers

What happens to tight junctions during tight junction dysfunction?

<p>They increase the permeability of the epithelial barrier.</p> Signup and view all the answers

What structure is formed when six connexins assemble together?

<p>Connexon</p> Signup and view all the answers

What type of molecules can diffuse through gap junctions?

<p>Ions and small molecules under 1000 daltons</p> Signup and view all the answers

Which type of junction helps in sealing neighboring cells to prevent leakage of extracellular molecules?

<p>Tight junction</p> Signup and view all the answers

In which type of muscle cells are gap junctions notably important for communication?

<p>Cardiac and smooth muscle</p> Signup and view all the answers

How do gap junctions influence metabolic activity between cells?

<p>By allowing direct transfer of nutrients and signals</p> Signup and view all the answers

Which of the following proteins primarily forms gap junctions?

<p>Connexin</p> Signup and view all the answers

What is the primary function of adherens junctions?

<p>Connecting the cytoskeleton of adjacent cells</p> Signup and view all the answers

How does the permeability of gap junctions vary between different tissues?

<p>It is influenced by the specific combinations of connexins forming the junctions.</p> Signup and view all the answers

What is the role of E-cadherin in the context of cancer?

<p>It acts as a tumor suppressor.</p> Signup and view all the answers

Which protein is primarily responsible for linking cadherins to the actin cytoskeleton?

<p>α-catenin</p> Signup and view all the answers

What do desmosomes primarily provide to epithelial tissues?

<p>Mechanical strength</p> Signup and view all the answers

Which of the following statements about hemidesmosomes is accurate?

<p>They anchor cells to the basal lamina.</p> Signup and view all the answers

Pemphigus is a disease resulting from autoantibodies targeting which component?

<p>Desmogleins</p> Signup and view all the answers

Which type of junction provides direct communication between the cytoplasm of adjacent cells?

<p>Gap junctions</p> Signup and view all the answers

What is the function of integrins in hemidesmosomes?

<p>To anchor cells to the basal lamina</p> Signup and view all the answers

Which proteins are primarily involved in the adhesion of desmosomes?

<p>Nonclassical cadherins</p> Signup and view all the answers

What type of binding occurs between occludin on adjacent cells within tight junctions?

<p>Homophilic binding</p> Signup and view all the answers

What is a hypothesized role of the first extracellular loop of claudin?

<p>Determines paracellular tightness</p> Signup and view all the answers

What is the primary function of Junction Adhesion Molecules (JAM)?

<p>Regulating the paracellular pathway</p> Signup and view all the answers

Which type of protein connects tight junctions to the actin cytoskeleton?

<p>Zonula occludens proteins</p> Signup and view all the answers

What directly contributes to tight junction dysfunction in Crohn's disease?

<p>Reduction of tight junction strands</p> Signup and view all the answers

What role does calcium play in the function of cadherins within adherens junctions?

<p>Facilitates binding</p> Signup and view all the answers

What distinguishes tight junctions from anchoring junctions in terms of strength?

<p>Anchoring junctions offer minimal adhesive strength</p> Signup and view all the answers

What is a key characteristic of desmosomes in relation to other junction types?

<p>They connect intermediate filaments</p> Signup and view all the answers

Which statement accurately describes the function of gap junctions in epithelial cells?

<p>They provide electrical and metabolic coupling between cells.</p> Signup and view all the answers

What is a key characteristic of hemidesmosomes in epithelial tissue?

<p>They anchor cells to the basal lamina.</p> Signup and view all the answers

Which protein family is primarily involved in the formation of adherens junctions?

<p>Cadherins</p> Signup and view all the answers

Which type of junction is characterized by its role in regulating paracellular transport?

<p>Tight junctions</p> Signup and view all the answers

What role do claudin proteins play in tight junctions?

<p>They determine the selectivity and tightness of the junctions.</p> Signup and view all the answers

What is the primary structural unit of gap junctions?

<p>Connexon</p> Signup and view all the answers

Which of the following best describes the function of gap junctions?

<p>They allow for direct electrical and metabolic coupling of adjacent cells.</p> Signup and view all the answers

What is the primary role of the larger first extracellular loop of claudin in tight junctions?

<p>Determining the paracellular tightness</p> Signup and view all the answers

How many different human connexin proteins have been identified?

<p>21</p> Signup and view all the answers

Which proteins link tight junctions to the actin cytoskeleton?

<p>Zonula occludens proteins</p> Signup and view all the answers

What is the significance of the differences in connexin composition in gap junctions across different tissues?

<p>It creates variations in channel permeability and regulation.</p> Signup and view all the answers

Which type of junction is primarily responsible for sealing cells together to prevent leakage in epithelial tissue?

<p>Tight junction</p> Signup and view all the answers

What is the molecular weight of Junction Adhesion Molecules (JAM)?

<p>~40kDa</p> Signup and view all the answers

Which component of gap junctions allows ions and small molecules to diffuse between adjacent cells?

<p>Connexon</p> Signup and view all the answers

What characterizes the tight junction dysfunction observed in Crohn's disease?

<p>Strand breaks and alterations in protein composition</p> Signup and view all the answers

What role do connexins play in the formation of gap junctions?

<p>They form the transmembrane channel allowing communication.</p> Signup and view all the answers

Why do cadherins require calcium in the extracellular medium?

<p>To effectively bind to each other</p> Signup and view all the answers

What type of binding occurs between occludin on adjacent cells?

<p>Homophilic binding</p> Signup and view all the answers

Which statement about the functionalities of gap junctions is correct?

<p>They allow for the exchange of nutrients and communication.</p> Signup and view all the answers

Which junction is primarily responsible for providing mechanical strength among epithelial cells?

<p>Adherens junctions</p> Signup and view all the answers

What is the effect of the shorter second extracellular loop of claudin on the paracellular cleft?

<p>Narrowing of the cleft</p> Signup and view all the answers

What role does β-catenin play in adherens junctions?

<p>It associates with actin filaments and regulates junction assembly.</p> Signup and view all the answers

Which type of protein do desmosomes primarily use to connect to intermediate filaments?

<p>Cadherins</p> Signup and view all the answers

What is one of the primary functions of hemidesmosomes?

<p>Anchor cells to the basal lamina.</p> Signup and view all the answers

What disease is characterized by the autoantibodies targeting desmogleins?

<p>Pemphigus</p> Signup and view all the answers

How does E-cadherin function in relation to tumors?

<p>It acts as a tumor suppressor and reduces invasiveness.</p> Signup and view all the answers

What is the function of gap junctions in cells?

<p>To provide direct connections between the cytoplasm of adjacent cells.</p> Signup and view all the answers

Which of the following best describes the structural difference between adherens junctions and desmosomes?

<p>Adherens junctions link to actin filaments, while desmosomes link to intermediate filaments.</p> Signup and view all the answers

What component is found in hemidesmosomes that helps anchor cells to the basal lamina?

<p>Integrin</p> Signup and view all the answers

Which statement correctly describes the role of tight junctions in epithelial cells?

<p>They create a barrier preventing the diffusion of lipids between membrane domains.</p> Signup and view all the answers

Which protein serves as a major component of tight junctions?

<p>Occludin</p> Signup and view all the answers

What type of transport do tight junctions primarily restrict?

<p>Paracellular transport of large molecules</p> Signup and view all the answers

How do tight junctions contribute to the polarization of epithelial cells?

<p>By preventing the movement of membrane proteins to the opposite surface</p> Signup and view all the answers

Which feature distinguishes tight junctions from other types of cell junctions?

<p>They contain transmembrane proteins that form seals.</p> Signup and view all the answers

In what way does occludin differ from claudin in the context of tight junctions?

<p>Occludin has a longer cytosolic region than claudin.</p> Signup and view all the answers

What is the primary function of the transmembrane proteins found in tight junctions?

<p>To form barriers that regulate substance passage</p> Signup and view all the answers

What type of connections do gap junctions provide between adjacent epithelial cells?

<p>Direct channels for intracellular communication</p> Signup and view all the answers

What is the primary function of tight junctions in epithelial cells?

<p>Prevent leakage between cells</p> Signup and view all the answers

Which type of junction is primarily responsible for connecting cells to the extracellular matrix?

<p>Hemidesmosomes</p> Signup and view all the answers

What type of proteins are involved in forming gap junctions?

<p>Connexins</p> Signup and view all the answers

Which junction provides minimal adhesive strength compared to others?

<p>Tight junctions</p> Signup and view all the answers

What is the role of cadherins in adherens junctions?

<p>Mediate cell-cell adhesion</p> Signup and view all the answers

Which protein is a major component of tight junctions?

<p>Occludin</p> Signup and view all the answers

What do tight junctions help to separate in the plasma membrane?

<p>Apical and basolateral domains</p> Signup and view all the answers

What type of transport do tight junctions primarily facilitate?

<p>Transcellular transport</p> Signup and view all the answers

What characteristic structure do tight junctions form between epithelial cells?

<p>Seals</p> Signup and view all the answers

Which type of junction is primarily responsible for enabling strong adhesion between epithelial cells?

<p>Desmosomes</p> Signup and view all the answers

Which domain of the plasma membrane do tight junctions primarily interact with?

<p>Apical domain</p> Signup and view all the answers

What is the weight of the Occludin protein typically associated with tight junctions?

<p>65 kDa</p> Signup and view all the answers

What role does E-cadherin play in the context of tumors?

<p>Acts as a tumor suppressor</p> Signup and view all the answers

How do desmosomes contribute to epithelial tissue?

<p>They enhance mechanical strength</p> Signup and view all the answers

What type of junction anchors cells to the basal lamina?

<p>Hemidesmosomes</p> Signup and view all the answers

What do gap junctions primarily provide between adjacent cells?

<p>Direct cytoplasmic connections</p> Signup and view all the answers

Which of the following proteins is found in desmosomes that link to intermediate filaments?

<p>Keratin</p> Signup and view all the answers

What is the function of β-catenin in adherens junctions?

<p>Regulates assembly of actin filaments</p> Signup and view all the answers

Which tissue structure is primarily linked by integrins in hemidesmosomes?

<p>Basal lamina</p> Signup and view all the answers

What structure is formed by six connexins in gap junctions?

<p>Connexon</p> Signup and view all the answers

Which of the following allows for the direct transmission of electrical signals between adjacent cells?

<p>Gap junctions</p> Signup and view all the answers

Which of the following is true regarding gap junctions?

<p>They can differ in properties based on connexin composition.</p> Signup and view all the answers

What is the main function of gap junctions in cardiac muscle cells?

<p>Direct transmission of electrical signals</p> Signup and view all the answers

How do gap junctions couple cells metabolically?

<p>By allowing nutrient flow between adjacent cells</p> Signup and view all the answers

What type of proteins make up the pore structure in gap junctions?

<p>Connexins</p> Signup and view all the answers

Which of these junctions is primarily responsible for sealing neighboring cells together to prevent leakage?

<p>Tight junctions</p> Signup and view all the answers

What is the role of claudin in tight junctions?

<p>Determines paracellular tightness and selective ion permeability</p> Signup and view all the answers

Which statement accurately describes Junction Adhesion Molecules (JAM)?

<p>They help regulate the paracellular pathway function</p> Signup and view all the answers

How do the intracellular tails of transmembrane proteins in tight junctions interact with the cytoskeleton?

<p>They bind to cytosolic proteins of the zonula occludens family</p> Signup and view all the answers

What distinguishes homophilic binding from heterophilic binding?

<p>Homophilic binding involves identical cell types</p> Signup and view all the answers

Which type of junction provides mechanical strength in epithelial tissues?

<p>Desmosomes</p> Signup and view all the answers

Which physiological condition is associated with tight junction dysfunction?

<p>Crohn's disease</p> Signup and view all the answers

What is a requirement for cadherins to effectively bind to one another?

<p>Calcium must be present in the extracellular medium</p> Signup and view all the answers

What is the primary feature of anchoring junctions?

<p>They connect the cytoskeleton to neighboring cells or the ECM</p> Signup and view all the answers

Study Notes

Eukaryotic Cell Biology: Cell Contacts/Cell Junctions

  • The lecture covers cell contacts/junctions in eukaryotic cells
  • Students will understand that cells are bound together by junctional complexes
  • Students will learn the different functions of these junctions
  • Students will identify the proteins involved in the junctions
  • Cells in many tissues, such as connective tissue, are separated by an extracellular matrix
  • In some tissues (epithelia), adjacent cells' plasma membranes are pressed together
  • This close contact enables the cells to function as an integrated unit

Levels of Cell Organization

  • Cellular organization progresses from cells to tissues, organs, organ systems, and finally to an organism
  • Cells form the building blocks for all advanced structures
  • The progression from single cell to complex organism reflects the organization of cells into larger functional units.

Cells Like to Stick Together

  • Cells in diverse tissues are either connected by an extracellular matrix or directly pressed together via their plasma membranes
  • Connective tissue components are often surrounded by a matrix (helps the tissue have structural integrity)
  • In epithelial cells, plasma membranes of adjacent cells form tight junctions that help tissues function as an integrated unit.

Types of Cell Junctions

  • There are five main types of cell junctions in epithelial cells:
    • Tight junctions
    • Adherens junctions
    • Desmosomes
    • Hemidesmosomes
    • Gap junctions

Tight Junctions

  • Tight junctions act as barriers between fluid compartments
  • They prevent molecules from passing between epithelial cells
  • Tight junctions separate the apical and basolateral domains of the plasma membrane
  • They regulate the passage of molecules across the epithelium
  • They are formed by transmembrane proteins (e.g., occludin, claudin, JAM)
  • Different tight junction proteins have different roles in regulating paracellular transport

Anchoring Junctions

  • Anchoring junctions connect the cytoskeleton of one cell to another or to the extracellular matrix (ECM)
  • They include adherens junctions and desmosomes
  • Adherens junctions connect to actin filaments while desmosomes are attached to intermediate filaments
  • They are vital for structural integrity and mechanical strength of tissues

Adherens Junctions

  • The main proteins involved are cell adhesion molecules (cadherins)
  • Cadherins bind to each other in the extracellular space, requiring calcium
  • These junctions are tightly linked to the intracellular actin cytoskeleton
  • A process called 'homophilic binding' strengthens the link

Desmosomes

  • Desmosomes form specialized plaques composed of proteins inside the cell
  • They link to intermediate filaments (e.g., keratin)
  • The strong adhesion of desmosomes makes tissues more resistant to mechanical stress
  • Certain skin and heart diseases can arise from desmosome defects

Hemidesmosomes

  • Hemidesmosomes do not link cells to each other; instead, they anchor cells to the basal lamina (part of the ECM)
  • Crucial proteins include transmembrane integrins that attach to intermediate filaments (e.g., keratin) and the ECM protein laminin
  • They are important for structural integrity and resistance to mechanical stress
  • They function in joining cells to an underlying basal lamina

Gap Junctions

  • Gap junctions are channels that connect the cytoplasm of adjacent cells
  • Made of connexin proteins which form connexons
  • They allow ions and small molecules to pass between cells
  • Gap junctions are important for cell-to-cell communication and coordination of activities between adjacent cells
  • They control the flow of small molecules, allowing coordination and communication between cardiac cells and smooth muscle cells.

Tight Junction Dysfunction

  • Tight junction dysfunction can lead to various health issues (e.g., Crohn's disease)
  • Such dysfunction can lead to abnormal passage of undigested food particles, toxins and bacteria

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Description

This quiz covers the essential aspects of cell contacts and junctions in eukaryotic cells. Students will explore the types of junctional complexes and their functions, as well as the proteins involved in these connections. Understanding how cells organize into tissues and systems is crucial for comprehending their integrated function.

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