Etest Method for Determining MIC
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Questions and Answers

What is the main difference between the Kirby-Bauer assay and the Etest method?

  • The Kirby-Bauer assay uses circular disks impregnated with antibiotics, while the Etest method uses plastic strips containing a gradient of antibiotics. (correct)
  • The Kirby-Bauer assay involves using plastic strips with antibiotic gradients, while the Etest method uses circular disks impregnated with antibiotics.
  • The Kirby-Bauer assay produces an elliptical zone of inhibition, while the Etest method produces a circular zone of inhibition.
  • The Kirby-Bauer assay allows concurrent comparison of multiple antimicrobials, while the Etest method does not.

    • How is the MIC determined using the Etest method?

  • By measuring the diameter of the circular zone of inhibition around the plastic strips.
  • By counting the number of bacterial colonies that grow around each plastic strip.
  • By the color change of the bacterial cells in contact with the plastic strips.
  • By observing the intersection of the elliptical zone with the gradient on the drug-containing strip. (correct)

    • Which method allows for the evaluation of multiple antimicrobials simultaneously on a single plate?

  • Dilution methods
  • Kirby-Bauer assay
  • Gram staining
  • Etest method (correct)

    • Why is an elliptical zone of inhibition observed in the Etest method?

    <p>Due to the direct relationship between drug diffusion rate and concentration.</p> Signup and view all the answers

    What allows for direct comparison of MIC values of different antimicrobials using the Etest method?

    <p>Placing multiple strips containing different antimicrobials on the same plate.</p> Signup and view all the answers

    How can researchers differentiate between different MIC values when using the Etest method?

    <p>By comparing the intersection points of different elliptical zones with their respective drug gradients.</p> Signup and view all the answers

    Study Notes

    Comparing Kirby-Bauer Assay and Etest Method

    • The main difference between the Kirby-Bauer assay and the Etest method is the way the Minimum Inhibitory Concentration (MIC) is determined: the Kirby-Bauer assay provides a qualitative result, while the Etest method provides a quantitative result.

    MIC Determination using Etest Method

    • In the Etest method, the MIC is determined by observing the point where the ellipse intersects the MIC scale on the strip.
    • The MIC value is read at the point where the inhibition ellipse edge intersects the strip.

    Evaluating Multiple Antimicrobials

    • The Etest method allows for the evaluation of multiple antimicrobials simultaneously on a single plate.

    Elliptical Zone of Inhibition

    • An elliptical zone of inhibition is observed in the Etest method because of the continuous concentration gradient of the antibiotic on the strip.

    Direct Comparison of MIC Values

    • The Etest method allows for direct comparison of MIC values of different antimicrobials because the strips have a standardized MIC scale.

    Differentiating MIC Values

    • Researchers can differentiate between different MIC values when using the Etest method by reading the MIC value at the point where the ellipse intersects the MIC scale on the strip.

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    Description

    Learn about the Etest method, which is used to determine the Minimum Inhibitory Concentration (MIC) of antibiotics. This method combines elements from the Kirby-Bauer assay and dilution methods, where plastic strips with a gradient of antibiotics are placed on an agar plate inoculated with bacteria.

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