Epithelial and Connective Tissue Quiz
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Questions and Answers

What function is primarily associated with epithelial tissue?

  • Transmitting nerve impulses
  • Covering surfaces and secreting substances (correct)
  • Facilitating body movements
  • Providing support and protection of tissues
  • Which domain of epithelial cells is exposed to the lumen or external environment?

  • Apical domain (correct)
  • Interstitial domain
  • Basal domain
  • Lateral domain
  • What component of the basement membrane is primarily responsible for cell adhesion?

  • Fibrinogen
  • Laminin (correct)
  • Reticular lamina
  • Collagen IV
  • Which type of epithelial tissue covers and protects underlying tissues?

    <p>Covering/lining epithelia (A)</p> Signup and view all the answers

    What structure separates epithelial tissue from underlying connective tissue?

    <p>Basement membrane (D)</p> Signup and view all the answers

    Which of the following statements about connective tissue is accurate?

    <p>Connective tissue supports and protects other tissues. (B)</p> Signup and view all the answers

    What is primarily found within the reticular lamina of the basement membrane?

    <p>Collagen III fibers (C)</p> Signup and view all the answers

    Which cell type is NOT part of epithelial structure?

    <p>Contractile cells (C)</p> Signup and view all the answers

    What is the primary advantage of using super resolution microscopy like STED?

    <p>It allows for higher resolution by using a secondary laser to repress fluorescent molecules. (C)</p> Signup and view all the answers

    Which microscopy technique is most suitable for observing the surface details of a metal sample?

    <p>Scanning electron microscopy (SEM) (B)</p> Signup and view all the answers

    What role does cholesterol play in the plasma membrane?

    <p>It modulates the density and fluidity of the membrane. (B)</p> Signup and view all the answers

    How does the structure of phospholipids contribute to the formation of the plasma membrane?

    <p>Their hydrophobic tails and polar heads form a bilayer. (A)</p> Signup and view all the answers

    What type of microscopy uses beams of electrons instead of visible light?

    <p>Electron microscopy (D)</p> Signup and view all the answers

    What is the primary function of proteins in the plasma membrane?

    <p>They provide recognition and signaling functions. (B)</p> Signup and view all the answers

    Which microscopy technique is best suited for focusing on a single detail within a sample?

    <p>Confocal microscope (C)</p> Signup and view all the answers

    What is a limitation of using a stereomicroscope?

    <p>It has a maximum magnification limit of 0.2 microns. (C)</p> Signup and view all the answers

    What is the primary function of phagocytosis?

    <p>To engulf bacteria or other particles (B)</p> Signup and view all the answers

    Which process involves the invagination of the cell membrane to create a vesicle containing extracellular fluid?

    <p>Pinocytosis (D)</p> Signup and view all the answers

    In receptor-mediated endocytosis, what initiates the aggregation of receptors leading to vesicle formation?

    <p>Ligand binding to the receptors (C)</p> Signup and view all the answers

    What role does clathrin play in the process of endocytosis?

    <p>It coats vesicles and aids in their formation (A)</p> Signup and view all the answers

    What is one consequence of exocytosis in a cell?

    <p>Increase of new membrane containing receptors (C)</p> Signup and view all the answers

    How does the binding of a ligand to its receptor affect signaling inside the cell?

    <p>It activates endocytosis of the complex (C)</p> Signup and view all the answers

    What is NOT a characteristic of coated vesicles?

    <p>They exclusively transport ligands inside the cell (C)</p> Signup and view all the answers

    What distinguishes receptor-mediated endocytosis from other types of endocytosis?

    <p>It is specific for certain ligands through binding (A)</p> Signup and view all the answers

    Which type of molecules can cross the membrane via simple diffusion?

    <p>Hydrophobic molecules (C)</p> Signup and view all the answers

    What role do channel proteins play in membrane transport?

    <p>They facilitate passive diffusion of specific molecules. (A)</p> Signup and view all the answers

    What characteristic defines the glycocalyx?

    <p>It is a sugar layer surrounding the membrane. (C)</p> Signup and view all the answers

    Which of the following statements is true regarding the Na/K pump?

    <p>It maintains the ion gradient by transporting Na out and K in. (D)</p> Signup and view all the answers

    How does the presence of oligosaccharide chains affect phospholipids and proteins in the membrane?

    <p>They stabilize the membrane. (D)</p> Signup and view all the answers

    What distinguishes active transport from passive transport?

    <p>Active transport requires energy to move molecules against their gradient. (D)</p> Signup and view all the answers

    What is a primary function of proteoglycans in the cellular environment?

    <p>They form a gel-like structure due to water interaction. (C)</p> Signup and view all the answers

    Which of the following molecules can diffuse freely across the cell membrane?

    <p>$CO_2$ (B)</p> Signup and view all the answers

    What happens to water when it freezes slowly, and why is this significant?

    <p>It creates larger crystals, which can obscure tissue detail. (C)</p> Signup and view all the answers

    Which stain is most effective for highlighting cellular cytoplasm structures?

    <p>Eosin stain (C)</p> Signup and view all the answers

    What is a basophilic structure and how is it best stained?

    <p>Structure that is best stained with alkaline dyes, highlighting DNA. (A)</p> Signup and view all the answers

    What is the function of PAS cell staining?

    <p>To delineate carbohydrates and certain mucins. (C)</p> Signup and view all the answers

    Why is the May-Grünwald-Giemsa method used specifically?

    <p>For analyzing blood and bone marrow cells. (D)</p> Signup and view all the answers

    What is the principle behind phase-contrast microscopy?

    <p>It combines small waves of light into a stronger wave when in phase. (C)</p> Signup and view all the answers

    What role do antibodies play in immunochemistry?

    <p>They bind to antigens at specific regions called epitopes. (C)</p> Signup and view all the answers

    What could be inferred about cytoplasm that shows basophilic characteristics?

    <p>It is rich in DNA and is best stained with basic dyes. (B)</p> Signup and view all the answers

    What is the primary purpose of exosomes in cellular processes?

    <p>To facilitate communication and transport of substances between cells (C)</p> Signup and view all the answers

    Which component of the cytoskeleton is responsible for forming the mitotic spindle during cell division?

    <p>Microtubules (B)</p> Signup and view all the answers

    What structure acts as an anchoring system for the primary cilium in quiescent cells?

    <p>Centriole (C)</p> Signup and view all the answers

    What type of signaling occurs in the primary cilium and why is it significant?

    <p>Intense signaling that captures various cellular signals (A)</p> Signup and view all the answers

    Which part of the cytoskeleton is formed by two strands of actin and is involved in cell motility?

    <p>Microfilaments (B)</p> Signup and view all the answers

    What is the relationship between myosin and microfilaments during cell migration?

    <p>Myosin pulls on actin microfilaments to generate tension (D)</p> Signup and view all the answers

    During exocytosis, what type of vesicle is released by the multivesicular bodies?

    <p>Smaller vesicles, each containing specific content (D)</p> Signup and view all the answers

    How do microtubules differ from microfilaments in structure?

    <p>Microtubules are hollow tubes made of tubulin, microfilaments are solid fibers made of actin (B)</p> Signup and view all the answers

    Flashcards

    Phase-contrast microscopy

    A type of microscopy that uses the principle of light wave interference to visualize unstained cells.

    H&E staining

    A staining technique that uses hematoxylin and eosin to highlight cell structures. Nuclei stain blue due to DNA's affinity for hematoxylin, while cytoplasm typically stains pink/red due to eosin.

    PAS (Periodic Acid-Schiff) staining

    A type of staining technique that highlights carbohydrates, such as glycogen and glycoproteins, with a magenta color.

    May-Grünwald-Giemsa staining

    A staining technique specifically used for examining blood and bone marrow cells.

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    Tissue sectioning

    The process of treating tissue samples to allow them to be cut into thin sections for microscopic examination.

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    Stain

    A substance that binds to specific structures or substances within cells, making them visible under a microscope.

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    Epitope

    The specific region on an antigen molecule that an antibody binds to

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    Paratope

    The region on an antibody molecule that binds to an epitope.

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    Confocal Microscope

    A type of microscope that focuses on one specific slice of the sample by using a filter with a hole that allows only one specific light to pass through.

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    Stereomicroscope

    A microscope that allows for the observation of 3D non-transparent objects. The light source comes from the top and illuminates the sample.

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    Super Resolution Microscopy: STORM

    A microscopy technique that uses lasers to excite fluorescent molecules randomly in a sample. Then, the excited dots are captured and used to reconstruct a high-resolution image.

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    Super Resolution Microscopy: STED

    A microscopy technique that uses two lasers to excite fluorescent molecules. The first laser excites, while the second laser de-excites the molecule, creating a restricted fluorescent area resulting in higher resolution.

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    Electron microscopy

    A type of microscopy that uses a beam of electrons instead of light to view samples.

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    Transmission Electron Microscopy (TEM)

    A type of electron microscopy where electrons pass through a thin section of the sample and create an image. The image is formed using magnetic lenses to bend the electrons.

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    Scanning Electron Microscopy (SEM)

    A type of electron microscopy that uses electrons to scan the surface of the sample. The reflected electrons are captured to create a 3D image.

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    Cell

    The basic unit of life in all living organisms.

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    Cell membrane

    A thin, flexible layer that surrounds all living cells. Controls what enters and exits the cell, helping to maintain its internal environment.

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    Oligosaccharide chains

    Sugar chains attached to lipids and proteins in the cell membrane. They help with cell recognition and signaling.

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    Lipid rafts

    Specialized regions in the cell membrane enriched with cholesterol and sphingolipids. They are more stable than the rest of the membrane.

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    Glycocalix

    A layer of sugar molecules that surrounds the cell membrane. It helps with cell recognition, signal transduction, and protection.

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    Proteoglycans

    Large molecules composed of glycosaminoglycans linked to proteins. They attract water and form a gel-like substance.

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    Hyaluronic acid

    A type of glycosaminoglycan that attracts water and creates volume. It forms a jelly-like layer around cells.

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    Simple diffusion

    The movement of molecules across a membrane from a region of high concentration to a region of low concentration. No energy required.

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    Channel diffusion

    The movement of molecules across a membrane through protein channels. No energy required.

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    Endocytosis

    A process where the cell membrane engulfs extracellular material, forming a vesicle that brings it into the cell.

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    Phagocytosis

    A type of endocytosis where the cell engulfs large particles, such as bacteria, into a vesicle called a phagosome.

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    Pinocytosis

    A type of endocytosis where the cell engulfs small droplets of fluid, like a tiny sip.

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    Receptor-mediated endocytosis

    A highly specific type of endocytosis where receptors on the cell surface bind to specific ligands, triggering the formation of a vesicle.

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    Exocytosis

    A process where a vesicle fuses with the cell membrane, releasing its contents outside the cell.

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    Coated vesicles

    Vesicles coated with proteins, like clathrin, that help with their function.

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    Clathrin-coated vesicles

    A type of coated vesicle, known for its clathrin coat that resembles a canister.

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    Cell signaling through vesicles

    The process where a ligand binds to its receptor on the cell surface, activating endocytosis of the complex. This triggers the formation of a vesicle, which can continue signaling pathways inside the cell.

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    Exosomes

    Tiny vesicles released by cells, containing a diverse mix of molecules like nucleic acids, ligands, and amino acids.

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    Cytoplasm

    The jelly-like substance inside a cell's membrane, containing water and proteins.

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    Cytoskeleton

    A network of filaments and tubules inside the cell, providing structural support, aiding movement, and facilitating cell division.

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    Microtubules

    Hollow tubes made of tubulin, involved in cell shape, transport, and division.

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    Centrosome

    A structure formed by two centrioles arranged perpendicularly to each other, serving as the organizing center for microtubules.

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    Primary Cilium

    A finger-like projection from the cell membrane, built from microtubules, involved in cell signaling.

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    Microfilaments

    Thin filaments formed by actin, involved in cell shape, movement, and muscle contraction.

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    Focal Adhesions

    Structures formed by proteins that anchor microfilaments to the extracellular environment, allowing cells to sense their surroundings and move.

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    What are the four main tissue types?

    The four primary tissue types make up all the organs in our body.

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    What is Epithelial Tissue?

    Epithelial tissue is a sheet of closely packed cells forming a lining on the surface or body cavities. It functions in covering, lining, and secretion.

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    What is Connective Tissue?

    Connective tissue is a specialized type of tissue that provides support and protection to organs. It contains cells scattered within an abundance of extracellular matrix.

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    What is Muscular Tissue?

    Muscular tissue is made of contractile cells, enabling body movement. It contains a moderate amount of extracellular matrix.

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    What is Nervous Tissue?

    Nervous tissue is composed of elongated cells and a small amount of matrix. It transmits nerve impulses throughout the body.

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    What is Covering/lining epithelia?

    Covering/lining epithelia form layers on surfaces and can act as a barrier, but also protect and absorb, like in the skin.

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    What is Secretory/glandular epithelia?

    Secretory/glandular epithelia form glands that produce and secrete substances, like hormones or digestive fluids.

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    What is a basement membrane?

    The basement membrane is a thin, specialized structure that attaches epithelial tissue to the underlying connective tissue. It acts as a barrier and provides structural support. There are three domains: apical, lateral and basal.

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    Study Notes

    Histology

    • Histology is the study of the microscopic structure of normal tissues, allowing understanding of how tissues are built for their functions.
    • Tissues are organized collections of cells with similar morphological characteristics (epithelial, muscular, nervous, connective).
    • Cell size ranges from 10-30 micrometers.
    • Microscopes are used to study cellular structure.

    Light Transmission Microscopy

    • Uses visible light passing through a sample.
    • Objective lenses magnify and project the illuminated image to the eyepiece.
    • Common magnifications are X4 (low), X10 (medium), and X40 (high) for detailed views.
    • Specimen must be transparent, blood is transparent allowing observance of top of the coverglass. Non-transparent tissues need sectioning before viewing.
    • Tissue sections are 2-dimensional images of 3-dimensional tissues, orientation varying on the plane of the cut.

    Tissue Preparation

    • Fixation: Preserves tissue structure by cross-linking proteins and inactivating enzymes.
    • Dehydration: Tissue is transferred to increasingly concentrated alcohol solutions.
    • Clearing: Alcohol is removed with a miscible organic solvent.
    • Infiltration: Tissue is placed in melted paraffin/wax until completely infiltrated.
    • Embedding: Paraffin-infiltrated tissue is placed in a mold and allowed to harden.
    • Trimming: Paraffin block is trimmed to expose the tissue for microtome sectioning.

    Tissue Staining

    • Sections are stained to make structures visible and to highlight specific features.
    • Hematoxylin and Eosin (H&E) is the most common stain, staining cell nuclei blue and cytoplasm pink/red.
    • Periodic Acid-Schiff (PAS) stains carbohydrates, such as glycogen and glycoproteins, magenta.

    Immunochemistry

    • A technique that uses antibodies to target specific molecules.
    • Antibodies are proteins produced by B-lymphocytes.
    • Antibodies can be labeled with fluorescent molecules to indicate target molecules, or enzymes to produce stains for visualization.

    Microscopy Techniques

    • Phase-contrast microscopy: Observe unstained samples without killing them. The different phases of light interacting with the object give a clearer picture of the object in the specimen.
    • Confocal microscopy: Focuses on one thin slice of a sample.
    • Stereo/dissecting microscope: 3D non-transparent objects, good for looking at big or non-transparent samples.
    • Super resolution microscopy: Methods like STORM and STED provide highly detailed images.
    • Electron microscopy: Uses electron beams instead of light; yields high magnification & resolution.
      • TEM (Transmission): electrons pass through the sample; gives 2D images of internal structure
      • SEM (Scanning): Electrons bounce off the sample; shows 3D surface features, usually of metallized samples.

    The Cell

    • The cell is the smallest unit of tissue.
    • The plasma membrane composed of phospholipids, cholesterol and proteins, is selectively permeable.
    • Hyaluronic acid retains water, creating a jelly-like substance.
    • Membrane transport includes simple diffusion, channels, and carrier proteins.

    Cytoskeleton

    • Microtubules: hollow tubes formed by tubulin.
    • Microfilaments: composed of actin.
    • Intermediate Filaments: provide structural support.

    Organelles

    • Ribosomes: synthesize proteins.
    • Endoplasmic reticulum: involved in protein synthesis and lipid metabolism (rough has ribosomes attached, smooth doesn't).
    • Golgi apparatus: modifies and packages secretions, in the form of cisterns.
    • Lysosomes: contain digestive enzymes that break down waste material.
    • Mitochondria: produce energy (ATP).
    • Peroxisomes: metabolize fatty acids and toxins.
    • Cellular inclusions and pigments
      • Lipid droplets store lipids as energy reserves.
      • Glycogen granules: aggregates of carbohydrate; store sugars (glucose).
      • Pigment deposits are structures like melanin which protect the nucleus from damage from light, or hemosiderin for iron.

    Cell Cycle

    • Describes the phases a cell goes through to grow and divide.
    • Interphase (stages G1, S, G2) precedes mitosis.
    • Mitosis has phases (prophase, metaphase, anaphase, and telophase) leading to the creation of two daughter cells.

    The Nucleus

    • Control center of the cell; houses genetic material (DNA).
    • Surrounded by a double membrane (nuclear envelope).
    • Nucleolus is a site where ribosomes are assembled.
    • Chromatin/Chromosomes are DNA organized with proteins (histones).

    Cells in Tissues (e.g., of the skin)

    • Epithelium: Forms a protective layer or secretes substances.
    • Connective tissue: Supports and connects different tissue types.
    • Muscle: Enables movement.
    • Nervous tissue: Transmits nerve impulses.

    Types of Epithelial Tissues

    • Covering (or lining) epithelia (covers surfaces) and secretory/glandular epithelia (produces secretions).
    • Shapes: squamous (flat), cuboidal (cube-shaped), columnar (rectangular).
    • Layers: simple (one layer), stratified (multiple layers).

    Gland Types

    • Exocrine glands: Secrete products through ducts (e.g. salivary glands).
    • Endocrine glands: Secrete products directly into the bloodstream (e.g. pituitary/thyroid glands).
    • Unicellular glands (individual secretory cells), such as goblet cells in the intestine.

    Cell Death

    • Necrosis: Traumatic or abnormal death of cells usually resulting inflammatory response.
    • Apoptosis: Programmed, controlled cell death, essential for development and tissue homeostasis, without inflammation.

    Bone Tissue

    • Mineralized connective tissue with cells (osteoblasts, osteocytes, osteoclasts) and a matrix.
    • Compact bone (cortical) and spongy (trabecular bone).

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    Histology PDF

    Description

    This quiz focuses on key concepts related to epithelial and connective tissues, their structures, functions, and the microscopy techniques used to study them. Test your knowledge on cell types, basement membranes, and the roles of various cellular components. Perfect for students of biology and histology.

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