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Questions and Answers
What is the minimum distance at which two distinct points of a specimen can still be seen as separate entities in microscopy?
Resolution
What is Numerical Aperture (NA) in microscopy related to?
Refractive index (n) of the medium and angular aperture of the objective
Who observed the Airy disk in 1835?
George Biddell Airy
What is Abbe's diffraction limit based on?
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How did Lord Rayleigh define the limit of resolution in a diffraction-limited system?
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What is the formula for resolution based on Abbe's criterion?
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What is the formula for Snell's Law?
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Define the critical angle in the context of total internal reflection (TIR).
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What are the requirements for total internal reflection (TIR) to occur?
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What is the formula for the critical angle in terms of refractive indices?
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In STED microscopy, how does the resolution improve within the 200 nm zone?
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What is the significance of the Rayleigh criterion in microscopy?
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Explain the concept of numerical aperture.
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What is the Airy disk in microscopy?
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Define Abbe's diffraction limit.
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What is a Diffraction pattern in microscopy?
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Explain the Rayleigh criterion in microscopy.
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How does numerical aperture affect the resolution of an optical system?
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