Enzymes and Protein Structure

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Questions and Answers

Which mineral is essential for the function of cytochrome?

  • Copper and Silver (Cu and Ag)
  • Magnesium (Mg)
  • Iron (Fe) (correct)
  • Copper (Cu)

What is the most abundant secondary structure observed in proteins?

  • Non-parallel beta-pleated sheet
  • Parallel beta-pleated sheet
  • Beta-pleated sheet
  • Alpha-helix (correct)

Who is credited with coining the term 'enzyme'?

  • Pasteur
  • Buchner
  • Kuhne (correct)
  • Urey Miller

In eukaryotes, where does beta-oxidation primarily take place?

<p>Mitochondria (C)</p> Signup and view all the answers

What is Koshland's theory of enzyme action commonly known as?

<p>Induced Fit Model (B)</p> Signup and view all the answers

Which of the following minerals plays a central role in the electron transport chain as part of cytochrome proteins?

<p>Iron (Fe) (D)</p> Signup and view all the answers

What structural characteristic distinguishes an alpha-helix from a beta-pleated sheet in proteins?

<p>Hydrogen bonding patterns (C)</p> Signup and view all the answers

What contribution did Kuhne make to the field of Biochemistry?

<p>Coining the term 'enzyme' (C)</p> Signup and view all the answers

Why is the compartmentalization of beta-oxidation within the mitochondria important for cellular function?

<p>To prevent damage to other cellular components (D)</p> Signup and view all the answers

How does the 'Induced Fit Model' differ from the 'Lock and Key Model' in explaining enzyme-substrate interactions?

<p>It suggests the enzyme's active site is flexible and adapts to the substrate (C)</p> Signup and view all the answers

Which of the following elements is directly involved in the redox reactions carried out by cytochromes?

<p>Iron (A)</p> Signup and view all the answers

What type of bonds are primarily responsible for stabilizing the alpha-helical structure of proteins?

<p>Hydrogen bonds (A)</p> Signup and view all the answers

What was a key contribution of Wilhelm Kuhne to the development of biochemistry, besides coining the term 'enzyme'?

<p>Isolating and characterizing trypsin (D)</p> Signup and view all the answers

If beta-oxidation were to occur unchecked in the cytoplasm, what potential consequence would it have on the cell?

<p>Uncontrolled release of energy and potential cellular damage (C)</p> Signup and view all the answers

In the 'Induced Fit Model,' what change occurs in the enzyme structure upon substrate binding?

<p>The enzyme's active site conforms to the substrate's shape (C)</p> Signup and view all the answers

How does iron contribute specifically to the function of cytochrome c oxidase in the electron transport chain?

<p>It facilitates the transfer of electrons to oxygen (C)</p> Signup and view all the answers

What would be the most immediate effect on protein structure if hydrogen bonds within an alpha-helix were disrupted?

<p>Unfolding of the helix (A)</p> Signup and view all the answers

What aspect of enzyme function did Kuhne's work primarily aim to elucidate when he coined the term 'enzyme'?

<p>Enzyme's catalytic role in biochemical reactions (D)</p> Signup and view all the answers

How does the location of beta-oxidation in mitochondria protect other cellular processes?

<p>By preventing the accumulation of toxic byproducts (B)</p> Signup and view all the answers

What key experimental evidence supports the 'Induced Fit Model' over the 'Lock and Key Model'?

<p>Crystals structures of enzymes with bound substrates showing conformational changes (A)</p> Signup and view all the answers

Which metal-containing protein facilitates the final transfer of electrons to oxygen in the mitochondrial electron transport chain?

<p>Cytochrome c oxidase (B)</p> Signup and view all the answers

What is the significance of hydrogen bonds in maintaining the secondary structure of proteins?

<p>They stabilize alpha-helices and beta-pleated sheets (C)</p> Signup and view all the answers

How did Wilhelm Kuhne's definition of enzymes influence further research in biochemistry?

<p>It stimulated the identification and characterization of numerous enzymes (B)</p> Signup and view all the answers

What role does compartmentalization play in the regulation of metabolic pathways, such as beta-oxidation?

<p>Separating conflicting processes and concentrating enzymes (A)</p> Signup and view all the answers

If an enzyme’s active site changed shape to accommodate the substrate after binding, which model of enzyme action would this support?

<p>The 'Induced Fit Model' (C)</p> Signup and view all the answers

Why is iron a suitable element for cytochromes involved in the electron transport chain?

<p>It can readily accept and donate electrons (D)</p> Signup and view all the answers

Why is the alpha-helix a common secondary structure in proteins found in biological systems?

<p>They provide stability and structural support (D)</p> Signup and view all the answers

Other than coining the term, what specific experimental approach did Kuhne pioneer that advanced the study of enzymes?

<p>Enzyme purification (B)</p> Signup and view all the answers

What is a significant disadvantage of performing beta-oxidation in the cytoplasm rather than the mitochondria?

<p>Greater cellular damage due to toxic byproducts (A)</p> Signup and view all the answers

Flashcards

Mineral in Cytochromes

Iron (Fe) is a mineral associated with cytochromes, playing a crucial role in electron transfer processes.

Common Protein Structure

The alpha-helix (α-helix) is the most common secondary structure found in proteins, characterized by its coiled shape stabilized by hydrogen bonds.

Who coined 'Enzyme'?

The term 'enzyme' was coined by Kuhne.

Location of Beta-oxidation

Beta-oxidation occurs in the mitochondria.

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Study Notes

  • Iron (Fe) is the mineral associated with cytochrome.
  • The most common secondary structure of proteins is the β-pleated sheet.
  • The term "enzyme" was coined by Kuhne.
  • β-oxidation occurs in the mitochondria.
  • Koshland's theory of enzyme action is examined.

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