Enzyme Specificity and Optimum Conditions Quiz

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What happens to the rate of reaction as substrate concentration increases?

It increases up to a certain point and then plateaus (correct)

It decreases continuously

It increases indefinitely

It decreases up to a certain point and then stabilizes

What is the significance of the saturation point in enzyme reactions?

It shows the enzyme is denatured

It leads to enzyme specificity

It indicates the enzyme is inactive

It marks the maximum rate of reaction (correct)

Which enzyme is being used in the practical to observe the effect of pH on activity?

Catalase

Lipase

Amylase (correct)

Pepsin

What color does the iodine solution turn when starch is present?

Blue-black Signup and view all the answers

What material is used to check for the presence of starch in the solution during the practical?

Iodine solution Signup and view all the answers

Why does increasing substrate concentration above the saturation point have no effect on the rate of reaction?

All active sites on enzymes are already occupied Signup and view all the answers

What is required for enzymes to catalyze reactions effectively?

Complementary shape between enzyme and substrate Signup and view all the answers

What is the purpose of using a Bunsen Burner and water beaker in the enzyme activity experiment described?

To maintain a constant temperature throughout the reaction as temperature is a control variable. Signup and view all the answers

What happens to enzyme activity if the temperature exceeds the optimum range in humans?

The enzyme is denatured Signup and view all the answers

Which color change indicates that starch is still present and has not been broken down in the enzyme activity experiment?

Blue-black Signup and view all the answers

Why is it essential for enzymes to have an optimum pH level?

To stabilize the enzyme structure Signup and view all the answers

Why is it important to repeat dropping the solution into wells containing iodine solution every 10 seconds during the enzyme activity experiment?

To observe the progress of starch breakdown over time. Signup and view all the answers

What effect does a high or low pH have on enzyme activity?

Leads to denaturation of the enzyme Signup and view all the answers

What impact does temperature have on enzyme activity?

Extreme temperatures can denature enzymes, affecting their function. Signup and view all the answers

Why does enzyme denaturation occur at extreme temperatures?

Due to breaking of bonds holding the enzyme together Signup and view all the answers

In the enzyme activity experiment, what does the optimal pH of amylase suggest?

This pH range accelerates starch breakdown by amylase. Signup and view all the answers

What is the consequence of an enzyme being denatured?

Loss of ability to catalyze reactions Signup and view all the answers

How do rate calculations contribute to understanding enzyme activity?

They help in assessing how quickly enzymes are working through measuring reaction speed. Signup and view all the answers