Enzyme Specificity and Optimum Conditions Quiz
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Questions and Answers

What happens to the rate of reaction as substrate concentration increases?

  • It increases up to a certain point and then plateaus (correct)
  • It decreases continuously
  • It increases indefinitely
  • It decreases up to a certain point and then stabilizes

What is the significance of the saturation point in enzyme reactions?

  • It shows the enzyme is denatured
  • It leads to enzyme specificity
  • It indicates the enzyme is inactive
  • It marks the maximum rate of reaction (correct)

Which enzyme is being used in the practical to observe the effect of pH on activity?

  • Catalase
  • Lipase
  • Amylase (correct)
  • Pepsin

What color does the iodine solution turn when starch is present?

<p>Blue-black (A)</p> Signup and view all the answers

What material is used to check for the presence of starch in the solution during the practical?

<p>Iodine solution (A)</p> Signup and view all the answers

Why does increasing substrate concentration above the saturation point have no effect on the rate of reaction?

<p>All active sites on enzymes are already occupied (C)</p> Signup and view all the answers

What is required for enzymes to catalyze reactions effectively?

<p>Complementary shape between enzyme and substrate (B)</p> Signup and view all the answers

What is the purpose of using a Bunsen Burner and water beaker in the enzyme activity experiment described?

<p>To maintain a constant temperature throughout the reaction as temperature is a control variable. (D)</p> Signup and view all the answers

What happens to enzyme activity if the temperature exceeds the optimum range in humans?

<p>The enzyme is denatured (B)</p> Signup and view all the answers

Which color change indicates that starch is still present and has not been broken down in the enzyme activity experiment?

<p>Blue-black (C)</p> Signup and view all the answers

Why is it essential for enzymes to have an optimum pH level?

<p>To stabilize the enzyme structure (B)</p> Signup and view all the answers

Why is it important to repeat dropping the solution into wells containing iodine solution every 10 seconds during the enzyme activity experiment?

<p>To observe the progress of starch breakdown over time. (A)</p> Signup and view all the answers

What effect does a high or low pH have on enzyme activity?

<p>Leads to denaturation of the enzyme (A)</p> Signup and view all the answers

What impact does temperature have on enzyme activity?

<p>Extreme temperatures can denature enzymes, affecting their function. (D)</p> Signup and view all the answers

Why does enzyme denaturation occur at extreme temperatures?

<p>Due to breaking of bonds holding the enzyme together (A)</p> Signup and view all the answers

In the enzyme activity experiment, what does the optimal pH of amylase suggest?

<p>This pH range accelerates starch breakdown by amylase. (C)</p> Signup and view all the answers

What is the consequence of an enzyme being denatured?

<p>Loss of ability to catalyze reactions (D)</p> Signup and view all the answers

How do rate calculations contribute to understanding enzyme activity?

<p>They help in assessing how quickly enzymes are working through measuring reaction speed. (C)</p> Signup and view all the answers

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