Enzyme Kinetics pre-learning

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Which type of reactions can Michaelis-Menten (M & M) kinetics be applied to?

Pseudo first order reactions (correct)

Third order reactions

Zero order reactions

Second order reactions

What information can be determined using Michaelis-Menten (M & M) kinetics?

The maximum rate of the reaction (correct)

The affinity of the enzyme for the substrate

The concentration of the enzyme

The temperature of the reaction

In Michaelis-Menten (M & M) kinetics, what happens to the rate if more substrate A is added in a reaction involving two substrates, A and B?

The rate increases (correct)

The rate decreases

The rate stays the same

It depends on the concentration of substrate B

In Michaelis-Menten (M & M) kinetics, what happens to the rate if more substrate B is added in a reaction involving two substrates, A and B?

The rate stays the same Signup and view all the answers

Which substrate is 'ignored' in terms of Michaelis-Menten (M & M) kinetics in the reaction: Fumarate + Malate + H2O -> Acetate + Acetylcholine + Choline + H2O?

Fumarate Signup and view all the answers

Why does the rate stop increasing as the concentration of substrate continues to increase in the Michaelis-Menten (M & M) kinetics graph?

The enzyme becomes saturated with substrate Signup and view all the answers

What is the relationship between the concentration of a single substrate and the rate of the reaction in first order reactions?

Directly proportional Signup and view all the answers

What type of reaction is depicted in the Michaelis-Menten (M & M) kinetics graph?

Pseudo first order reaction Signup and view all the answers

What does the Michaelis-Menten (M & M) kinetics equation allow us to determine about an enzyme?

The affinity of the enzyme for the substrate Signup and view all the answers

What is the maximum rate in terms of Michaelis-Menten (M & M) kinetics?

The rate at which the enzyme becomes saturated with substrate Signup and view all the answers

Which equation represents Michaelis-Menten Kinetics?

Vo = Vmax[S] / ([S]+KM) Signup and view all the answers

What does Km indicate in enzyme kinetics?

Enzyme affinity Signup and view all the answers

What does Vmax represent in enzyme kinetics?

Maximum rate Signup and view all the answers

What type of graphing technique creates a straight line graph in enzyme kinetics?

Lineweaver-Burk Signup and view all the answers

What does the Y-intercept of a Lineweaver-Burk plot represent?

1/Vmax Signup and view all the answers

What does the X-intercept of a Lineweaver-Burk plot represent?

<ul> <li>1/Km</li> </ul> Signup and view all the answers

Which enzyme has the highest Vmax value?

A Signup and view all the answers

Which enzyme has the highest Km value?

C Signup and view all the answers

Which enzyme has the best affinity?

B Signup and view all the answers

Which of the following is NOT a characteristic of zero-order kinetics?

Rate is proportional to enzyme concentration Signup and view all the answers

Which type of reactions can Michaelis-Menten (M & M) kinetics be applied to?

First-order reactions Signup and view all the answers

What does Km indicate in enzyme kinetics?

The concentration of the substrate at half of the maximum rate Signup and view all the answers

What information can be determined using Michaelis-Menten (M & M) kinetics?

All of the above Signup and view all the answers

Why does the rate stop increasing as the concentration of substrate continues to increase in the Michaelis-Menten (M & M) kinetics graph?

The enzyme becomes saturated with substrate Signup and view all the answers

What types of reactions are depicted in the examples given?

First-order reactions Signup and view all the answers

Which substrate is 'ignored' in terms of Michaelis-Menten (M & M) kinetics in the reaction: Fumarate + Malate + H2O -> Acetate + Acetylcholine + Choline + H2O?

Malate Signup and view all the answers

What do we use the Michaelis-Menten (M & M) kinetics equation for?

All of the above Signup and view all the answers

What happens to the rate if more substrate A is added in a reaction involving two substrates, A and B?

The rate increases Signup and view all the answers

What happens to the rate if more substrate B is added in a reaction involving two substrates, A and B?

The rate decreases Signup and view all the answers

What does the Y-intercept of a Lineweaver-Burk plot represent?

The concentration of the enzyme at half of the maximum rate Signup and view all the answers

What does the X-intercept of a Lineweaver-Burk plot represent?

The concentration of the substrate at half of the maximum rate Signup and view all the answers

Which of the following is true about the Michaelis-Menten (M & M) kinetics equation?

It allows us to determine the initial velocity (Vo) of the reaction. Signup and view all the answers

Which of the following is true about the Lineweaver-Burk plot?

The X-intercept represents the enzyme affinity (Km). Signup and view all the answers

Which of the following enzymes has the highest Vmax value?

Enzyme C Signup and view all the answers

Which of the following enzymes has the highest Km value?

Enzyme B Signup and view all the answers

Which of the following enzymes has the best affinity?

Enzyme A Signup and view all the answers

Which of the following is true about the relationship between substrate concentration and reaction rate in first order reactions?

As substrate concentration increases, reaction rate also increases. Signup and view all the answers

Which of the following is NOT a characteristic of zero-order kinetics?

Reaction rate is proportional to substrate concentration. Signup and view all the answers

Which of the following is true about the Y-intercept of a Lineweaver-Burk plot?

It represents the reciprocal of the maximum rate (1/Vmax). Signup and view all the answers

Which of the following is true about the X-intercept of a Lineweaver-Burk plot?

It represents the enzyme affinity (Km). Signup and view all the answers

Which of the following is true about the Michaelis-Menten (M & M) kinetics graph?

It is a graph of [S] against reaction rate. Signup and view all the answers

Enzyme Kinetics pre-learning

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Questions and Answers

Which type of reactions can Michaelis-Menten (M & M) kinetics be applied to?

What information can be determined using Michaelis-Menten (M & M) kinetics?

In Michaelis-Menten (M & M) kinetics, what happens to the rate if more substrate A is added in a reaction involving two substrates, A and B?

In Michaelis-Menten (M & M) kinetics, what happens to the rate if more substrate B is added in a reaction involving two substrates, A and B?

Which substrate is 'ignored' in terms of Michaelis-Menten (M & M) kinetics in the reaction: Fumarate + Malate + H2O -> Acetate + Acetylcholine + Choline + H2O?

Why does the rate stop increasing as the concentration of substrate continues to increase in the Michaelis-Menten (M & M) kinetics graph?

What is the relationship between the concentration of a single substrate and the rate of the reaction in first order reactions?

What type of reaction is depicted in the Michaelis-Menten (M & M) kinetics graph?

What does the Michaelis-Menten (M & M) kinetics equation allow us to determine about an enzyme?

What is the maximum rate in terms of Michaelis-Menten (M & M) kinetics?

Which equation represents Michaelis-Menten Kinetics?

What does Km indicate in enzyme kinetics?

What does Vmax represent in enzyme kinetics?

What type of graphing technique creates a straight line graph in enzyme kinetics?

What does the Y-intercept of a Lineweaver-Burk plot represent?