Enzyme Kinetics pre-learning
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Questions and Answers

Which type of reactions can Michaelis-Menten (M & M) kinetics be applied to?

  • Pseudo first order reactions (correct)
  • Third order reactions
  • Zero order reactions
  • Second order reactions

    • What information can be determined using Michaelis-Menten (M & M) kinetics?

  • The maximum rate of the reaction (correct)
  • The affinity of the enzyme for the substrate
  • The concentration of the enzyme
  • The temperature of the reaction

    • In Michaelis-Menten (M & M) kinetics, what happens to the rate if more substrate A is added in a reaction involving two substrates, A and B?

  • The rate increases (correct)
  • The rate decreases
  • The rate stays the same
  • It depends on the concentration of substrate B

    • In Michaelis-Menten (M & M) kinetics, what happens to the rate if more substrate B is added in a reaction involving two substrates, A and B?

    <p>The rate stays the same</p> Signup and view all the answers

    Which substrate is 'ignored' in terms of Michaelis-Menten (M & M) kinetics in the reaction: Fumarate + Malate + H2O -> Acetate + Acetylcholine + Choline + H2O?

    <p>Fumarate</p> Signup and view all the answers

    Why does the rate stop increasing as the concentration of substrate continues to increase in the Michaelis-Menten (M & M) kinetics graph?

    <p>The enzyme becomes saturated with substrate</p> Signup and view all the answers

    What is the relationship between the concentration of a single substrate and the rate of the reaction in first order reactions?

    <p>Directly proportional</p> Signup and view all the answers

    What type of reaction is depicted in the Michaelis-Menten (M & M) kinetics graph?

    <p>Pseudo first order reaction</p> Signup and view all the answers

    What does the Michaelis-Menten (M & M) kinetics equation allow us to determine about an enzyme?

    <p>The affinity of the enzyme for the substrate</p> Signup and view all the answers

    What is the maximum rate in terms of Michaelis-Menten (M & M) kinetics?

    <p>The rate at which the enzyme becomes saturated with substrate</p> Signup and view all the answers

    Which equation represents Michaelis-Menten Kinetics?

    <p>Vo = Vmax[S] / ([S]+KM)</p> Signup and view all the answers

    What does Km indicate in enzyme kinetics?

    <p>Enzyme affinity</p> Signup and view all the answers

    What does Vmax represent in enzyme kinetics?

    <p>Maximum rate</p> Signup and view all the answers

    What type of graphing technique creates a straight line graph in enzyme kinetics?

    <p>Lineweaver-Burk</p> Signup and view all the answers

    What does the Y-intercept of a Lineweaver-Burk plot represent?

    <p>1/Vmax</p> Signup and view all the answers

    What does the X-intercept of a Lineweaver-Burk plot represent?

    <ul> <li>1/Km</li> </ul> Signup and view all the answers

    Which enzyme has the highest Vmax value?

    <p>A</p> Signup and view all the answers

    Which enzyme has the highest Km value?

    <p>C</p> Signup and view all the answers

    Which enzyme has the best affinity?

    <p>B</p> Signup and view all the answers

    Which of the following is NOT a characteristic of zero-order kinetics?

    <p>Rate is proportional to enzyme concentration</p> Signup and view all the answers

    Which type of reactions can Michaelis-Menten (M & M) kinetics be applied to?

    <p>First-order reactions</p> Signup and view all the answers

    What does Km indicate in enzyme kinetics?

    <p>The concentration of the substrate at half of the maximum rate</p> Signup and view all the answers

    What information can be determined using Michaelis-Menten (M & M) kinetics?

    <p>All of the above</p> Signup and view all the answers

    Why does the rate stop increasing as the concentration of substrate continues to increase in the Michaelis-Menten (M & M) kinetics graph?

    <p>The enzyme becomes saturated with substrate</p> Signup and view all the answers

    What types of reactions are depicted in the examples given?

    <p>First-order reactions</p> Signup and view all the answers

    Which substrate is 'ignored' in terms of Michaelis-Menten (M & M) kinetics in the reaction: Fumarate + Malate + H2O -> Acetate + Acetylcholine + Choline + H2O?

    <p>Malate</p> Signup and view all the answers

    What do we use the Michaelis-Menten (M & M) kinetics equation for?

    <p>All of the above</p> Signup and view all the answers

    What happens to the rate if more substrate A is added in a reaction involving two substrates, A and B?

    <p>The rate increases</p> Signup and view all the answers

    What happens to the rate if more substrate B is added in a reaction involving two substrates, A and B?

    <p>The rate decreases</p> Signup and view all the answers

    What does the Y-intercept of a Lineweaver-Burk plot represent?

    <p>The concentration of the enzyme at half of the maximum rate</p> Signup and view all the answers

    What does the X-intercept of a Lineweaver-Burk plot represent?

    <p>The concentration of the substrate at half of the maximum rate</p> Signup and view all the answers

    Which of the following is true about the Michaelis-Menten (M & M) kinetics equation?

    <p>It allows us to determine the initial velocity (Vo) of the reaction.</p> Signup and view all the answers

    Which of the following is true about the Lineweaver-Burk plot?

    <p>The X-intercept represents the enzyme affinity (Km).</p> Signup and view all the answers

    Which of the following enzymes has the highest Vmax value?

    <p>Enzyme C</p> Signup and view all the answers

    Which of the following enzymes has the highest Km value?

    <p>Enzyme B</p> Signup and view all the answers

    Which of the following enzymes has the best affinity?

    <p>Enzyme A</p> Signup and view all the answers

    Which of the following is true about the relationship between substrate concentration and reaction rate in first order reactions?

    <p>As substrate concentration increases, reaction rate also increases.</p> Signup and view all the answers

    Which of the following is NOT a characteristic of zero-order kinetics?

    <p>Reaction rate is proportional to substrate concentration.</p> Signup and view all the answers

    Which of the following is true about the Y-intercept of a Lineweaver-Burk plot?

    <p>It represents the reciprocal of the maximum rate (1/Vmax).</p> Signup and view all the answers

    Which of the following is true about the X-intercept of a Lineweaver-Burk plot?

    <p>It represents the enzyme affinity (Km).</p> Signup and view all the answers

    Which of the following is true about the Michaelis-Menten (M & M) kinetics graph?

    <p>It is a graph of [S] against reaction rate.</p> Signup and view all the answers

    Which enzyme has the highest affinity?

    <p>Enzyme A</p> Signup and view all the answers

    Which enzyme has the highest Vmax value?

    <p>Enzyme C</p> Signup and view all the answers

    Which enzyme has the highest Km value?

    <p>Enzyme D</p> Signup and view all the answers

    Which enzyme has the best affinity?

    <p>Enzyme A</p> Signup and view all the answers

    Which enzyme has the highest Vmax value?

    <p>Enzyme C</p> Signup and view all the answers

    Which enzyme has the highest Km value?

    <p>Enzyme D</p> Signup and view all the answers

    Which enzyme has the best affinity?

    <p>Enzyme A</p> Signup and view all the answers

    Which enzyme has the highest Vmax value?

    <p>Enzyme C</p> Signup and view all the answers

    Which enzyme has the highest Km value?

    <p>Enzyme D</p> Signup and view all the answers

    Which enzyme has the best affinity?

    <p>Enzyme A</p> Signup and view all the answers

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