Enzyme Kinetics pre-learning
51 Questions
1 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Which type of reactions can Michaelis-Menten (M & M) kinetics be applied to?

  • Pseudo first order reactions (correct)
  • Third order reactions
  • Zero order reactions
  • Second order reactions

What information can be determined using Michaelis-Menten (M & M) kinetics?

  • The maximum rate of the reaction (correct)
  • The affinity of the enzyme for the substrate
  • The concentration of the enzyme
  • The temperature of the reaction

In Michaelis-Menten (M & M) kinetics, what happens to the rate if more substrate A is added in a reaction involving two substrates, A and B?

  • The rate increases (correct)
  • The rate decreases
  • The rate stays the same
  • It depends on the concentration of substrate B

In Michaelis-Menten (M & M) kinetics, what happens to the rate if more substrate B is added in a reaction involving two substrates, A and B?

<p>The rate stays the same (D)</p> Signup and view all the answers

Which substrate is 'ignored' in terms of Michaelis-Menten (M & M) kinetics in the reaction: Fumarate + Malate + H2O -> Acetate + Acetylcholine + Choline + H2O?

<p>Fumarate (B)</p> Signup and view all the answers

Why does the rate stop increasing as the concentration of substrate continues to increase in the Michaelis-Menten (M & M) kinetics graph?

<p>The enzyme becomes saturated with substrate (A)</p> Signup and view all the answers

What is the relationship between the concentration of a single substrate and the rate of the reaction in first order reactions?

<p>Directly proportional (C)</p> Signup and view all the answers

What type of reaction is depicted in the Michaelis-Menten (M & M) kinetics graph?

<p>Pseudo first order reaction (D)</p> Signup and view all the answers

What does the Michaelis-Menten (M & M) kinetics equation allow us to determine about an enzyme?

<p>The affinity of the enzyme for the substrate (D)</p> Signup and view all the answers

What is the maximum rate in terms of Michaelis-Menten (M & M) kinetics?

<p>The rate at which the enzyme becomes saturated with substrate (A)</p> Signup and view all the answers

Which equation represents Michaelis-Menten Kinetics?

<p>Vo = Vmax[S] / ([S]+KM) (B)</p> Signup and view all the answers

What does Km indicate in enzyme kinetics?

<p>Enzyme affinity (D)</p> Signup and view all the answers

What does Vmax represent in enzyme kinetics?

<p>Maximum rate (A)</p> Signup and view all the answers

What type of graphing technique creates a straight line graph in enzyme kinetics?

<p>Lineweaver-Burk (B)</p> Signup and view all the answers

What does the Y-intercept of a Lineweaver-Burk plot represent?

<p>1/Vmax (A)</p> Signup and view all the answers

What does the X-intercept of a Lineweaver-Burk plot represent?

<ul> <li>1/Km (A)</li> </ul> Signup and view all the answers

Which enzyme has the highest Vmax value?

<p>A (C)</p> Signup and view all the answers

Which enzyme has the highest Km value?

<p>C (D)</p> Signup and view all the answers

Which enzyme has the best affinity?

<p>B (B)</p> Signup and view all the answers

Which of the following is NOT a characteristic of zero-order kinetics?

<p>Rate is proportional to enzyme concentration (A)</p> Signup and view all the answers

Which type of reactions can Michaelis-Menten (M & M) kinetics be applied to?

<p>First-order reactions (A)</p> Signup and view all the answers

What does Km indicate in enzyme kinetics?

<p>The concentration of the substrate at half of the maximum rate (B)</p> Signup and view all the answers

What information can be determined using Michaelis-Menten (M & M) kinetics?

<p>All of the above (D)</p> Signup and view all the answers

Why does the rate stop increasing as the concentration of substrate continues to increase in the Michaelis-Menten (M & M) kinetics graph?

<p>The enzyme becomes saturated with substrate (B)</p> Signup and view all the answers

What types of reactions are depicted in the examples given?

<p>First-order reactions (B)</p> Signup and view all the answers

Which substrate is 'ignored' in terms of Michaelis-Menten (M & M) kinetics in the reaction: Fumarate + Malate + H2O -> Acetate + Acetylcholine + Choline + H2O?

<p>Malate (A)</p> Signup and view all the answers

What do we use the Michaelis-Menten (M & M) kinetics equation for?

<p>All of the above (D)</p> Signup and view all the answers

What happens to the rate if more substrate A is added in a reaction involving two substrates, A and B?

<p>The rate increases (A)</p> Signup and view all the answers

What happens to the rate if more substrate B is added in a reaction involving two substrates, A and B?

<p>The rate decreases (B)</p> Signup and view all the answers

What does the Y-intercept of a Lineweaver-Burk plot represent?

<p>The concentration of the enzyme at half of the maximum rate (A)</p> Signup and view all the answers

What does the X-intercept of a Lineweaver-Burk plot represent?

<p>The concentration of the substrate at half of the maximum rate (A)</p> Signup and view all the answers

Which of the following is true about the Michaelis-Menten (M & M) kinetics equation?

<p>It allows us to determine the initial velocity (Vo) of the reaction. (C)</p> Signup and view all the answers

Which of the following is true about the Lineweaver-Burk plot?

<p>The X-intercept represents the enzyme affinity (Km). (A)</p> Signup and view all the answers

Which of the following enzymes has the highest Vmax value?

<p>Enzyme C (D)</p> Signup and view all the answers

Which of the following enzymes has the highest Km value?

<p>Enzyme B (B)</p> Signup and view all the answers

Which of the following enzymes has the best affinity?

<p>Enzyme A (A)</p> Signup and view all the answers

Which of the following is true about the relationship between substrate concentration and reaction rate in first order reactions?

<p>As substrate concentration increases, reaction rate also increases. (A)</p> Signup and view all the answers

Which of the following is NOT a characteristic of zero-order kinetics?

<p>Reaction rate is proportional to substrate concentration. (D)</p> Signup and view all the answers

Which of the following is true about the Y-intercept of a Lineweaver-Burk plot?

<p>It represents the reciprocal of the maximum rate (1/Vmax). (D)</p> Signup and view all the answers

Which of the following is true about the X-intercept of a Lineweaver-Burk plot?

<p>It represents the enzyme affinity (Km). (A)</p> Signup and view all the answers

Which of the following is true about the Michaelis-Menten (M & M) kinetics graph?

<p>It is a graph of [S] against reaction rate. (B)</p> Signup and view all the answers

Which enzyme has the highest affinity?

<p>Enzyme A (D)</p> Signup and view all the answers

Which enzyme has the highest Vmax value?

<p>Enzyme C (A)</p> Signup and view all the answers

Which enzyme has the highest Km value?

<p>Enzyme D (A)</p> Signup and view all the answers

Which enzyme has the best affinity?

<p>Enzyme A (B)</p> Signup and view all the answers

Which enzyme has the highest Vmax value?

<p>Enzyme C (C)</p> Signup and view all the answers

Which enzyme has the highest Km value?

<p>Enzyme D (C)</p> Signup and view all the answers

Which enzyme has the best affinity?

<p>Enzyme A (B)</p> Signup and view all the answers

Which enzyme has the highest Vmax value?

<p>Enzyme C (B)</p> Signup and view all the answers

Which enzyme has the highest Km value?

<p>Enzyme D (A)</p> Signup and view all the answers

Which enzyme has the best affinity?

<p>Enzyme A (A)</p> Signup and view all the answers

More Like This

Use Quizgecko on...
Browser
Open
Browser
Browser