Enzyme Kinetics and Km in Biochemistry

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What is the rate of reaction when it is independent of substrate concentration?

Second order

Third order

Zero order (correct)

First order

What does Km represent in terms of enzyme-substrate affinity?

Substrate concentration at Vmax

Affinity for a substrate (correct)

Enzyme activity

Rate of reaction

How is a low Km value related to the affinity between enzyme and substrate?

Moderate affinity

No affinity

Neutral affinity

Strong affinity (correct)

What does a high Km value indicate about the affinity between enzyme and substrate?

Weak affinity Signup and view all the answers

In which range are the Km values typically found for the majority of enzymes?

$10^{-5}$ to $10^{-2}$ moles Signup and view all the answers

Is the Km value dependent on the concentration of the enzyme?

Independent of enzyme concentration Signup and view all the answers

What is the enzyme unit based on?

Amount of substrate that converts to product in one minute Signup and view all the answers

What does turnover number measure?

The number of moles of substrate per mole of enzyme Signup and view all the answers

What does specific activity measure?

Activity of the enzyme per milligram of protein Signup and view all the answers

Which factor does not affect the rate of enzyme-catalyzed reactions?

Product concentration Signup and view all the answers

At what temperature do most human enzymes start to denature?

40°C Signup and view all the answers

How do enzymes speed up biochemical reactions?

By decreasing activation energy required for reactions Signup and view all the answers

What does the Michaelis-Menten equation describe?

The order of reaction with respect to substrate concentration Signup and view all the answers

What happens when the enzyme concentration is halved in a reaction?

The Vmax remains the same Signup and view all the answers

When is the rate of reaction said to be first order with respect to substrate?

When [S] is much less than Vmax Signup and view all the answers

Enzyme Kinetics and Km in Biochemistry

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Questions and Answers

What is the rate of reaction when it is independent of substrate concentration?

What does Km represent in terms of enzyme-substrate affinity?

How is a low Km value related to the affinity between enzyme and substrate?

What does a high Km value indicate about the affinity between enzyme and substrate?

In which range are the Km values typically found for the majority of enzymes?

Is the Km value dependent on the concentration of the enzyme?

What is the enzyme unit based on?

What does turnover number measure?

What does specific activity measure?

Which factor does not affect the rate of enzyme-catalyzed reactions?

At what temperature do most human enzymes start to denature?

How do enzymes speed up biochemical reactions?

What does the Michaelis-Menten equation describe?

What happens when the enzyme concentration is halved in a reaction?

When is the rate of reaction said to be first order with respect to substrate?

Which parameter in the Michaelis-Menten equation is a measure of enzyme efficiency?

What happens to the velocity of the reaction when [S] is much greater than Km?

How does halving the enzyme concentration affect Vmax in a reaction?

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