Enzyme Kinetics and Km in Biochemistry
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Questions and Answers

What is the rate of reaction when it is independent of substrate concentration?

  • Second order
  • Third order
  • Zero order (correct)
  • First order

    • What does Km represent in terms of enzyme-substrate affinity?

  • Substrate concentration at Vmax
  • Affinity for a substrate (correct)
  • Enzyme activity
  • Rate of reaction

    • How is a low Km value related to the affinity between enzyme and substrate?

  • Moderate affinity
  • No affinity
  • Neutral affinity
  • Strong affinity (correct)

    • What does a high Km value indicate about the affinity between enzyme and substrate?

    <p>Weak affinity</p> Signup and view all the answers

    In which range are the Km values typically found for the majority of enzymes?

    <p>$10^{-5}$ to $10^{-2}$ moles</p> Signup and view all the answers

    Is the Km value dependent on the concentration of the enzyme?

    <p>Independent of enzyme concentration</p> Signup and view all the answers

    What is the enzyme unit based on?

    <p>Amount of substrate that converts to product in one minute</p> Signup and view all the answers

    What does turnover number measure?

    <p>The number of moles of substrate per mole of enzyme</p> Signup and view all the answers

    What does specific activity measure?

    <p>Activity of the enzyme per milligram of protein</p> Signup and view all the answers

    Which factor does not affect the rate of enzyme-catalyzed reactions?

    <p>Product concentration</p> Signup and view all the answers

    At what temperature do most human enzymes start to denature?

    <p>40°C</p> Signup and view all the answers

    How do enzymes speed up biochemical reactions?

    <p>By decreasing activation energy required for reactions</p> Signup and view all the answers

    What does the Michaelis-Menten equation describe?

    <p>The order of reaction with respect to substrate concentration</p> Signup and view all the answers

    What happens when the enzyme concentration is halved in a reaction?

    <p>The Vmax remains the same</p> Signup and view all the answers

    When is the rate of reaction said to be first order with respect to substrate?

    <p>When [S] is much less than Vmax</p> Signup and view all the answers

    Which parameter in the Michaelis-Menten equation is a measure of enzyme efficiency?

    <p>Km</p> Signup and view all the answers

    What happens to the velocity of the reaction when [S] is much greater than Km?

    <p>It is constant and equal to Vmax</p> Signup and view all the answers

    How does halving the enzyme concentration affect Vmax in a reaction?

    <p>Vmax decreases by half</p> Signup and view all the answers

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