Enzyme Kinetics and Km in Biochemistry

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Questions and Answers

What is the rate of reaction when it is independent of substrate concentration?

  • Second order
  • Third order
  • Zero order (correct)
  • First order

What does Km represent in terms of enzyme-substrate affinity?

  • Substrate concentration at Vmax
  • Affinity for a substrate (correct)
  • Enzyme activity
  • Rate of reaction

How is a low Km value related to the affinity between enzyme and substrate?

  • Moderate affinity
  • No affinity
  • Neutral affinity
  • Strong affinity (correct)

What does a high Km value indicate about the affinity between enzyme and substrate?

<p>Weak affinity (A)</p> Signup and view all the answers

In which range are the Km values typically found for the majority of enzymes?

<p>$10^{-5}$ to $10^{-2}$ moles (A)</p> Signup and view all the answers

Is the Km value dependent on the concentration of the enzyme?

<p>Independent of enzyme concentration (C)</p> Signup and view all the answers

What is the enzyme unit based on?

<p>Amount of substrate that converts to product in one minute (C)</p> Signup and view all the answers

What does turnover number measure?

<p>The number of moles of substrate per mole of enzyme (B)</p> Signup and view all the answers

What does specific activity measure?

<p>Activity of the enzyme per milligram of protein (D)</p> Signup and view all the answers

Which factor does not affect the rate of enzyme-catalyzed reactions?

<p>Product concentration (D)</p> Signup and view all the answers

At what temperature do most human enzymes start to denature?

<p>40°C (A)</p> Signup and view all the answers

How do enzymes speed up biochemical reactions?

<p>By decreasing activation energy required for reactions (A)</p> Signup and view all the answers

What does the Michaelis-Menten equation describe?

<p>The order of reaction with respect to substrate concentration (B)</p> Signup and view all the answers

What happens when the enzyme concentration is halved in a reaction?

<p>The Vmax remains the same (B)</p> Signup and view all the answers

When is the rate of reaction said to be first order with respect to substrate?

<p>When [S] is much less than Vmax (D)</p> Signup and view all the answers

Which parameter in the Michaelis-Menten equation is a measure of enzyme efficiency?

<p>Km (C)</p> Signup and view all the answers

What happens to the velocity of the reaction when [S] is much greater than Km?

<p>It is constant and equal to Vmax (C)</p> Signup and view all the answers

How does halving the enzyme concentration affect Vmax in a reaction?

<p>Vmax decreases by half (C)</p> Signup and view all the answers

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