Enzyme Kinetics and Inhibition
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Questions and Answers

What is the substrate concentration at the maximal velocity of an enzyme-catalyzed reaction?

  • The reaction's equilibrium point
  • Half the maximal velocity of the reaction (correct)
  • Zero velocity of the reaction
  • The maximal velocity of the reaction

    • Uncompetitive reversible inhibition of an enzyme results from inhibitor binding to which of the following?

  • The free enzyme only
  • The free enzyme and the enzyme-substrate complex (correct)
  • The free substrate
  • The enzyme-substrate complex only

    • In a plot of 1/V against 1/[S] for an enzyme-catalyzed reaction, what does the presence of a competitive inhibitor alter?

  • The intercept on the 1/[S] axis (correct)
  • The intercept on the 1/V axis
  • The Vmax
  • The curvature of the plot

    • What is the overall ΔG'° for the two successive reactions converting glucose 1-phosphate to fructose 6-phosphate?

    <p>-5.4 kJ/mol</p> Signup and view all the answers

    Where and under what conditions is acetyl-CoA produced from pyruvate?

    <p>The mitochondria, only under aerobic conditions</p> Signup and view all the answers

    What is true about the glycolytic pathway?

    <p>Endergonic reactions are all driven forward by being coupled to exergonic reactions</p> Signup and view all the answers

    What is the primary function of glycogen phosphorylase in the mobilization of glucose from glycogen?

    <p>Release free glucose 1-phosphate molecules</p> Signup and view all the answers

    What is a characteristic of the reaction that converts pyruvate to acetyl-CoA and CO2?

    <p>It involves the participation of thiamine</p> Signup and view all the answers

    Where are triacylglycerols acted upon during the digestion and absorption of dietary lipids?

    <p>In the small intestine by the action of lipases</p> Signup and view all the answers

    What happens to glucose 6-phosphate in the liver?

    <p>It is dephosphorylated to maintain blood glucose levels</p> Signup and view all the answers

    How many NADH molecules are produced per glucose molecule during glycolysis?

    <p>Two</p> Signup and view all the answers

    What is produced from the addition of inorganic phosphate to ADP during ATP production?

    <p>ATP</p> Signup and view all the answers

    What is a characteristic of oligomeric proteins?

    <p>They can further associate into large fibres</p> Signup and view all the answers

    Which of the following is a type of protein found in red blood cells?

    <p>Oxygen transport protein found in red blood cells</p> Signup and view all the answers

    What is the role of enzyme catalysts in biochemical reactions?

    <p>To lower the activation energy for the conversion of substrate to product</p> Signup and view all the answers

    What is a characteristic of allosteric enzymes?

    <p>Their activity is not influenced by modulators</p> Signup and view all the answers

    What is the Vmax for an enzyme catalysed reaction?

    <p>The maximal velocity of an enzyme catalysed reaction</p> Signup and view all the answers

    What is a characteristic of a subunit in a protein?

    <p>It may have non-protein prosthetic groups</p> Signup and view all the answers

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