Enzyme Inhibitors and Lineweaver-Burk Plots

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What is the effect of a competitive inhibitor on the apparent Km of an enzyme?

It is increased (correct)

It is decreased

It is unchanged

It becomes zero

What happens to the reaction velocity when the concentration of substrate is increased in the presence of a competitive inhibitor?

It decreases

It reaches Vmax (correct)

It becomes zero

It remains unaffected

What is the effect of non-competitive inhibitors on the binding of substrate to enzyme?

They bind to the active site of the enzyme

They have no effect on the binding of substrate to enzyme (correct)

They increase the binding of substrate to enzyme

They decrease the binding of substrate to enzyme

What is the point of intersection between the plots of inhibited and uninhibited reactions in a Lineweaver-Burk plot?

The y-axis at 1/Vmax (C) Signup and view all the answers

What is the result of increasing the substrate concentration in the presence of uncompetitive inhibitors?

The inhibition is increased (A) Signup and view all the answers

What is a potential limitation of enzyme activity measurements?

All of the above (D) Signup and view all the answers

What is the purpose of maintaining a constant pH during enzyme concentration measurements?

To ensure accurate enzyme activity measurements (B) Signup and view all the answers

What is the effect of non-competitive inhibitors on Vmax?

It is decreased (B) Signup and view all the answers

What is the effect of non-competitive inhibitors on Km?

It is unchanged (C) Signup and view all the answers

What is the principle of the fixed-time assay method?

Reactants are combined, reaction proceeds for a designated time, and then the reaction is stopped (D) Signup and view all the answers

How do uncompetitive inhibitors differ from competitive inhibitors?

Uncompetitive inhibitors are not overcome by increasing substrate concentration, while competitive inhibitors are (B) Signup and view all the answers

What is the advantage of continuous-monitoring or kinetic assays?

They can be used to measure substrate depletion (A) Signup and view all the answers

What is the effect of increasing substrate concentration on competitive inhibition?

Inhibition is partially reversed (D) Signup and view all the answers

What type of bond is formed between a reversible inhibitor and an enzyme?

Noncovalent bond (D) Signup and view all the answers

What happens when the concentration of substrate is significantly higher than the concentration of a competitive inhibitor?

Inhibition is partially reversed (A) Signup and view all the answers

What is the characteristic of non-competitive inhibitors?

They bind to an allosteric site (B) Signup and view all the answers

What is the effect of increasing substrate concentration on uncompetitive inhibition?

Inhibition is increased (A) Signup and view all the answers

What is the characteristic of mixed inhibitors?

They bind to either E or ES complex (D) Signup and view all the answers