Enzymatic Activity of rRNA in Peptide Bonds

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Questions and Answers

Peptide bond formation is catalyzed by the enzymatic activity of an rRNA moleculeg

  • true (correct)
  • false

Because there are three different possible reading frames in a messenger RNA (mRNA) molecule, most mRNAs can be translated in a cell into three different proteins

False (B)

Refer to the mRNA sequence: 5'-AUGAGACUUACCGAA-3'. What would the anticodon look like if the second nucleotide of the fourth codon was mutated to U?

  • 3'-AUG-5'
  • 3'-UAG-5' (correct)
  • 3'-TAG-5'
  • 3'-AAC-5'

If a mutation blocked the function of the signal recognition particle, making it unable to bind signal sequence, what would result?

<p>All proteins would be translated on free ribosomes in the cytoplasm. (D)</p> Signup and view all the answers

Based on the information given in the previous slide, the amino acids in histone proteins cannot be replaced without disrupting the protein's structure.

<p>True (A)</p> Signup and view all the answers

Which force or bonding can contribute to a protein's tertiary structure?

<p>All of these choices are correct. (A)</p> Signup and view all the answers

If this domain folds in one of the two orientations shown, which is more likely, the orientation in Figure A or the orientation in Figure B?

<p>Figure B (B)</p> Signup and view all the answers

Of the following choices, which can be a product of a catabolic reaction?

<p>an amino acid like tryptophan (B)</p> Signup and view all the answers

Which of the following statements is a consequence of the first law of thermodynamics?

<p>The energy at the start of a reaction equals the sum of the energy at the end of a reaction plus the energy released as heat and disorder. (D)</p> Signup and view all the answers

Why does ADP have less potential energy than ATP?

<p>because ADP has only two phosphate groups (C)</p> Signup and view all the answers

Which of the following reactions is most likely to be exergonic?

<p>the conversion of protein from food into amino acids (B)</p> Signup and view all the answers

The amino acids that contribute to catalysis in the active site of an enzyme do not have to be located close to each other in the primary sequence of the protein (the linear sequence of amino acids).

<p>False (B)</p> Signup and view all the answers

Which of the following characteristics would you predict a substrate to possess in order for it to bind the active site of the enzyme?

<p>hydrophilic, with a negative charge (B)</p> Signup and view all the answers

According to the scenario, which of the statements is true of the compound?

<p>The compound is an allosteric inhibitor. (A)</p> Signup and view all the answers

Glycolysis results in the partial oxidation of glucose to pyruvate. Which of the following describes the partial oxidation?

<p>In the process of the conversion of glucose to pyruvate, some potential energy is transferred to NADH and ATP. (C)</p> Signup and view all the answers

In which stage of aerobic cellular respiration is the most energy transferred from chemical bonds in the fuel molecule to bonds in other high energy molecules?

<p>the citric acid cycle (C)</p> Signup and view all the answers

If an energy source is available, the citric acid cycle can run in reverse in some organisms.

<p>True (A)</p> Signup and view all the answers

When considering the transfer and capture of potential energy derived from glucose during cellular respiration, which molecule receives and uses that potential energy for various cellular functions? This molecule is sometimes called "Energy currency of cells".

<p>ATP (A)</p> Signup and view all the answers

The energy from the movement of electrons through the electron transport chain is directly used to synthesize ATP.

<p>False (B)</p> Signup and view all the answers

You would expect beta-oxidation to occur in a cell when pyruvate concentrations are low.

<p>True (A)</p> Signup and view all the answers

Why is the absorption and capture of light energy by chlorophyll molecules in plants much more efficient than light energy absorption and capture by a solution of chlorophyll molecules in the lab?

<p>In the plant, the energy is transferred between molecules. (C)</p> Signup and view all the answers

At what point do the electrons have the greatest amount of potential energy?

<p>after capturing photon energy in the reaction center of photosystem I (C)</p> Signup and view all the answers

What result would you predict if a plant was exposed to a toxin that made the thylakoid membranes permeable to protons?

<p>The proton gradient across the thylakoid membrane would be eliminated. (B)</p> Signup and view all the answers

Which phase of the Calvin cycle would be impacted first by the absence of light energy?

<p>the reduction of 3-phosphoglycerate (B)</p> Signup and view all the answers

How many times must each of the reactions in the Calvin cycle take place in order to complete the synthesis of one 3-carbon triose phosphate sugar molecule?

<p>3 (D)</p> Signup and view all the answers

How does the plant cell protect itself from harmful reactive oxygen species, which form by the interaction of high-energy electrons and O₂ molecules?

<p>by producing antioxidants (B)</p> Signup and view all the answers

Plants that possess high levels of xanthophylls are better suited to growth in bright sunlight.

<p>True (A)</p> Signup and view all the answers

To the right of the dotted line, where is the lagging strand synthesized?

<p>top strand (C)</p> Signup and view all the answers

What feature of double-stranded DNA makes it necessary to have a leading strand and a lagging strand during replication?

<p>the antiparallel orientation of the strands (B)</p> Signup and view all the answers

DNA replication of small circular molecules usually starts at a single origin of replication and proceeds bidirectionally -- that is, with two replication forks proceeding in opposite directions from the origin of replication. The time required for replication would be longer if replication of such a molecule were unidirectional rather than bidirectional.

<p>True (A)</p> Signup and view all the answers

A lack of telomerase activity limits what?

<p>the number of times a cell can divide (D)</p> Signup and view all the answers

Colchicine is a drug that blocks the assembly of microtubules. If dividing cells are treated with colchicine, at what stage of mitosis would you predict the arrest of cell division to occur?

<p>metaphase (E)</p> Signup and view all the answers

A skin cell in G₂ of interphase has _____ as much DNA as it had in G₁.

<p>twice (D)</p> Signup and view all the answers

Which one of the choices would most likely contribute to uncontrolled cell proliferation (that is, cancer)?

<p>a mutant CDK that was active in the absence of its cyclin binding partner (C)</p> Signup and view all the answers

If a cell underwent mitosis and its daughter cells were immediately exposed to chemicals that damaged the DNA, at which stage of the cell cycle checkpoint would you predict the arrest of cell division to occur?

<p>G₂ checkpoint (D)</p> Signup and view all the answers

Which of the following would be the most likely to lead to the development of cancer?

<p>the activation of an oncogene and the inactivation of a tumor suppressor gene (B)</p> Signup and view all the answers

Which of the following statements might explain hemophilia in a girl born to parents with normal blood-clotting phenotypes? (Hint: Any X from the father will have a normal blood-clotting gene)

<p>Nondisjunction during sperm formation resulted in her receiving no sex chromosome from her father and an X chromosome from her mother; she is XO. (A)</p> Signup and view all the answers

Flashcards

Peptide bond formation

Formation of a peptide bond is catalyzed by rRNA.

mRNA reading frames

mRNA can't be translated into three different proteins.

mRNA sequence mutation

Changing the 2nd nucleotide in the 4th codon of 5'-AUGAGACUUACCGAA-3' to 'U'.

Signal recognition particle (SRP) mutation

All proteins will be produced on free ribosomes in cytoplasm if SRP is non-functional.

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Histone protein mutation

Replacing amino acids in histone proteins may disrupt the protein structure.

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Protein tertiary structure

Van der Waals, disulfide bond, hydrogen, and ionic bonds create a protein's tertiary structure.

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Domain folding

Figure A is more likely for domain folding because of less repulsion.

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Catabolic reaction product

An amino acid like tryptophan is a product of a catabolic reaction.

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First Law of Thermodynamics

Energy in a reaction doesn't disappear; transforms from one form to another.

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ADP vs ATP

ADP has less potential energy because of fewer phosphate groups.

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Exergonic reaction

Conversion of protein to amino acid is likely an exergonic reaction, releasing energy.

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Enzyme active site amino acids

Amino acids in the active site, despite not being necessarily adjacent in the primary sequence, need to be close together.

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Substrate characteristics

Hydrophilic substrates with negative charges are likely to bind to enzyme active sites.

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Compound effect

The compound acts as an allosteric inhibitor that binds to an enzyme's non-active site, changing shape

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Redox reaction

Electrons are lost during reduction.

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Glycolysis partial oxidation

Glucose to pyruvate transfers potential energy to NADH and ATP.

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Aerobic respiration energy transfer

Citric acid cycle is where most energy in fuel molecule is transfered.

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Citric acid cycle reversal

Citric acid cycle can run backward with enough energy source available in some organisms

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Cellular respiration energy currency

ATP is the molecule that receives and uses potential energy for cellular functions.

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Electron transport chain ATP synthesis

Electron transport chain does not directly produce ATP

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β-oxidation and pyruvate concentration

β-oxidation is more likely to occur when pyruvate concentrations are low.

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Chlorophyll efficiency

Chlorophyll capture light energy more efficiently in plants than in solutions due to energy transfer between molecules.

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Electron potential energy highest

Electron potential energy is greatest after capturing photon energy in photosystem I.

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Thylakoid membrane permeability

A toxin making thylakoid membranes permeable to protons eliminates the proton gradient.

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Calvin cycle phase impacted by light

Calvin cycle's reduction phase is first impacted by light.

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Calvin Cycle repeat for triose phosphate

The reactions in the Calvin cycle must be repeated six times to produce a three-carbon triose phosphate sugar molecule.

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Plant protection from ROS

Plants produce antioxidants to protect themselves from ROS

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Xanthophylls and sunlight

Plants with high xanthophyll levels tolerate bright sunlight better.

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Lagging strand synthesis location

The lagging strand is synthesized on the top strand.

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DNA replication antiparallel strands

Antiparallel nature of DNA strands makes leading and lagging strands necessary during replication.

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Circular DNA replication

DNA replication of small circular molecules usually begins at one origin and proceeds bidirectionally.

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Telomerase and cell division

Telomere shortening limits the cell's ability to divide.

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Colchicine and mitosis

Colchicine prevents microtubule formation, arresting cell division during prophase.

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G2 and DNA content

A skin cell in G2 has twice the amount of DNA it had in G1.

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Uncontrolled cell proliferation & cancer

A mutant CDK active independently of cyclin is a risk factor for cancer.

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DNA damage cell cycle arrest

Cell division arrests at the G1 checkpoint if daughter cells have DNA damage after mitosis.

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Cancer development risk

Activation of an oncogene and inactivation of a tumor suppressor increase cancer risk.

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Hemophilia in girls

Nondisjunction during sperm formation might lead to hemophilia in girls with no inherited mutations.

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