Enterobacteriaceae Identification Quiz
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Enterobacteriaceae Identification Quiz

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Questions and Answers

What characteristic differentiates coliform bacteria from non-coliform bacteria?

  • Coliform bacteria ferment lactose to produce acid and gas (correct)
  • Coliform bacteria can produce gas from glucose
  • Coliform bacteria are always pathogenic
  • Coliform bacteria do not ferment lactose

    • Which of the following organisms is classified as a non-coliform?

  • Salmonella (correct)
  • Escherichia coli
  • Enterobacter spp.
  • Klebsiella spp.

    • What is the primary purpose of serological testing in pathogen detection?

  • To identify specific antigens in a sample (correct)
  • To culture bacteria from urine samples
  • To determine lactose fermentation ability
  • To classify bacteria based on morphology

    • Which test is commonly used to differentiate among Enterobacteriaceae species?

    <p>Triple Sugar Iron (TSI) test</p> Signup and view all the answers

    In the fermentation process, what compounds do coliform bacteria primarily produce?

    <p>Acid and gas</p> Signup and view all the answers

    What is typically the first step in urine formation?

    <p>Filtration</p> Signup and view all the answers

    What characteristic of Pseudomonas aeruginosa makes it a common pathogen in hospital settings?

    <p>It is resistant to many antibiotics</p> Signup and view all the answers

    What type of culture media is used primarily for isolating Pseudomonas aeruginosa?

    <p>Cetrimide agar</p> Signup and view all the answers

    What characteristic of Eosin Methylene Blue (EMB) Agar distinguishes lactose fermenters?

    <p>Blue-black or metallic sheen colonies</p> Signup and view all the answers

    Which of the following accurately describes the function of MacConkey Agar?

    <p>Contains bile salts and crystal violet to inhibit Gram positive bacteria</p> Signup and view all the answers

    What does a positive result in the Triple Sugar Iron (TSI) test indicate?

    <p>Production of hydrogen sulfide</p> Signup and view all the answers

    Which group of bacteria is characterized as coliform?

    <p>Lactose fermenting Gram negative bacteria</p> Signup and view all the answers

    What is the primary aim of serological testing for pathogens?

    <p>To detect specific antibodies or antigens in a sample</p> Signup and view all the answers

    What growth characteristic is associated with Pseudomonas aeruginosa on Cetrimide Agar?

    <p>Production of a greenish pigment</p> Signup and view all the answers

    Which biochemical test is incorporated in the IMVIC series?

    <p>Methyl Red test</p> Signup and view all the answers

    Which statement accurately compares coliform and non-coliform bacteria?

    <p>Coliform can ferment lactose, while non-coliform bacteria cannot.</p> Signup and view all the answers

    What characteristic coloration indicates the presence of Pseudomonas aeruginosa on Cetrimide Agar?

    <p>Yellow-green to blue color colonies</p> Signup and view all the answers

    What does the IMVIC test 'V' suggest about a bacterium?

    <p>Presence of acetoin</p> Signup and view all the answers

    Which test is used to assess a bacterium's ability to ferment sugars and produce hydrogen sulfide?

    <p>Triple Sugar Iron (TSI) Test</p> Signup and view all the answers

    Which of the following describes a feature that differentiates lactose fermenters from non-lactose fermenters on agar plates?

    <p>Red colonies indicate lactose fermenters</p> Signup and view all the answers

    What limitation might be observed with Cetrimide Agar when testing for Pseudomonas aeruginosa?

    <p>Some enterics may exhibit slight yellowing of the medium</p> Signup and view all the answers

    Which IMVIC biochemical test is specifically used to detect the enzyme tryptophanase?

    <p>Indole Test</p> Signup and view all the answers

    What type of bacteria would likely produce a pink pigmentation on Cetrimide Agar?

    <p>Serratia species</p> Signup and view all the answers

    What is a common application of serological testing in microbiology?

    <p>Identifying pathogen-specific antigens</p> Signup and view all the answers

    Study Notes

    Enterobacteriaceae and Gram-Negative Intestinal Pathogens

    • Enterobacteriaceae are a family of gram-negative bacteria commonly found in the intestines of warm-blooded animals, including humans.
    • Coliforms are lactose fermenting enterobacteriaceae and are used as indicators of fecal contamination in water and food.
    • Coliform examples include Escherichia coli, Klebsiella spp., and Enterobacter spp.
    • Non-Coliform enterobacteriaceae are gram-negative but do not ferment lactose to produce gas.
    • Non-Coliform examples include Salmonella, Shigella, and Yersinia and are often pathogenic, causing gastrointestinal diseases.

    Enterobacteriaceae Identification

    • EMB (Eosin Methylene Blue) Agar is used to isolate and differentiate gram-negative bacteria.
      • Metallic sheen/blue-black colonies: Lactose fermenters
      • Dark purple colonies: Slow lactose fermenters
      • Pink colonies: Non-lactose fermenters
    • MacConkey Agar is used to isolate and differentiate gram-negative bacteria.
      • Pink colonies: Lactose fermenters
      • Colorless colonies: Non-lactose fermenters
    • Salmonella-Shigella Agar is used to isolate Salmonella and Shigella.
      • Red colonies: Lactose fermenters
      • Colorless colonies: Non-lactose fermenters
    • Cetrimide Agar is used to isolate Pseudomonas aeruginosa.
      • Yellow-green to blue color colonies: Indicate the production of pyocyanin by P. aeruginosa

    Biochemical Testing for Enterobacteriaceae

    • IMVIC is a series of four biochemical tests used to identify enterobacteriaceae.
      • Indole: Tests for the presence of tryptophanase, an enzyme that cleaves tryptophan to produce indole.
      • Methyl Red (MR): Tests for the production of mixed acids from glucose fermentation.
      • Voges-Proskauer (VP): Tests for the presence of acetoin, suggesting the bacterium follows the 2,3-butanediol fermentation pathway.
      • Citrate: Tests for the ability to use citrate as the only carbon source.
    • Triple Sugar Iron (TSI) Agar: Tests for the fermentation of sugars (glucose, lactose, and sucrose), production of gas, and the presence of hydrogen sulfide (H2S).
    • Urea Agar: Tests for the presence of urease, an enzyme that hydrolyzes urea into ammonia and carbon dioxide.

    Urine Culture Techniques and Urinary Tract Pathogens

    • Urine is a sterile waste product formed in the kidneys, stored in the bladder, and excreted through the urethra.
    • Urine culture techniques are used to diagnose urinary tract infections (UTIs).
    • Common urinary tract pathogens include Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Staphylococcus saprophyticus.
    • Causes of UTIs in Females: Shorter urethra, closer proximity to the anus, and sexual activity.

    Disease Outbreaks Associated with Enterobacteriaceae

    • Food and Waterborne Diseases/Intoxication
    • Animal Contact Disease Outbreaks
    • Antibiotic-Associated Diarrhea
    • Antimicrobial Resistance Pathogens
    • Cases with Immunocompromised Individuals

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    Related Documents

    PH 152 Medical and Public Health Microbiology Post Lab Notes PDF

    Description

    Test your knowledge on the Enterobacteriaceae family and the identification of gram-negative intestinal pathogens. Learn about the characteristics of coliforms and non-coliforms, as well as the significance of EMB and MacConkey agar in microbiology. This quiz will challenge your understanding of these critical microorganisms.

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