Enamel Composition and Properties

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Questions and Answers

What percentage of enamel's composition is made up of inorganic substances?

  • 75%
  • 50%
  • 25%
  • 96% (correct)

Which property of enamel is described as being the hardest calcified tissue in the human body?

  • Hardness (correct)
  • Thickness
  • Brittleness
  • Color

How does the diameter of an enamel rod change from the dentino-enamel junction to the outer enamel surface?

  • It remains constant
  • It decreases
  • It doubles in size
  • It increases by a ratio of 1:2 (correct)

What is the primary reason for enamel's brittleness?

<p>Loss of healthy dentine beneath (C)</p> Signup and view all the answers

What describes the course of an enamel rod near the outer surface of enamel?

<p>Straight (A)</p> Signup and view all the answers

What variation occurs in the direction of enamel rods in deciduous teeth?

<p>They vary from vertical, to oblique, to horizontal (D)</p> Signup and view all the answers

How many enamel rods can be found in the upper first permanent molar?

<p>12 million (C)</p> Signup and view all the answers

What type of structure is the primary unit of enamel?

<p>Enamel rod (A)</p> Signup and view all the answers

What is the primary product secreted by ameloblasts at the end of enamel formation?

<p>Primary enamel cuticle (D)</p> Signup and view all the answers

Which type of enamel lamella is caused by poorly calcified enamel rod and inter-rod substance?

<p>Enamel lamella type (A) (C)</p> Signup and view all the answers

Enamel tufts primarily arise from which area in the structure of the tooth?

<p>Dentin-enamel junction (D.E.J.) (B)</p> Signup and view all the answers

Which type of enamel lamella occurs after eruption and contains organic substances from saliva?

<p>Enamel lamella type (C) (C)</p> Signup and view all the answers

What is the appearance of enamel spindles in ground sections?

<p>Pointed or rounded processes (C)</p> Signup and view all the answers

Enamel lamellae may act as what in the dental structure?

<p>Caries spread paths (B)</p> Signup and view all the answers

What is the typical shape of enamel tufts in cross-sections?

<p>Tufts of grass (B)</p> Signup and view all the answers

What happens to the cells at the depth of a type (B) enamel lamella?

<p>They undergo degeneration (A)</p> Signup and view all the answers

What is the primary function of the prism sheath in enamel rods?

<p>To resist decalcification (B)</p> Signup and view all the answers

What optical phenomenon is caused by the changes in rod direction within enamel?

<p>Hunter-Shreger bands (C)</p> Signup and view all the answers

How are Hunter-Shreger bands visually distinguished when viewed using reflected light?

<p>They alternate between dark zones and light zones (B)</p> Signup and view all the answers

What do the cross striations in enamel represent?

<p>Daily rhythmic increments of enamel formation (A)</p> Signup and view all the answers

What are the incremental lines of Retzius primarily associated with?

<p>Rhythmic formation of enamel (D)</p> Signup and view all the answers

Where do the incremental lines of Retzius terminate in the enamel structure?

<p>In a series of transverse depressions called perikimata (C)</p> Signup and view all the answers

What characterizes the neonatal lines found in teeth?

<p>They separate enamel formed before birth from after birth (D)</p> Signup and view all the answers

What is primarily responsible for the higher refractive index of the inter-rod substance in enamel?

<p>It has a denser arrangement than enamel rods (A)</p> Signup and view all the answers

What is the primary characteristic of structure-less enamel?

<p>It is more mineralized than normal enamel. (B)</p> Signup and view all the answers

How are perikymata represented on the enamel surface?

<p>As transverse wave-like grooves. (A)</p> Signup and view all the answers

Where are rod ends typically found in enamel?

<p>Shallow at the cervical region. (A)</p> Signup and view all the answers

What happens to the cracks in enamel after decalcification?

<p>They disappear completely. (C)</p> Signup and view all the answers

Which stage of ameloblasts involves the differentiation of odontoblasts?

<p>Organizing stage. (C)</p> Signup and view all the answers

What defines the morphogenic stage of ameloblasts?

<p>The inner enamel epithelium arranges on the basement membrane. (B)</p> Signup and view all the answers

What is the significance of a fibrilar cementum in enamel structure?

<p>It forms due to residual dental epithelium before eruption. (B)</p> Signup and view all the answers

How many perikymata are typically found per millimeter in the cervical area of enamel?

<p>30 per mm. (A)</p> Signup and view all the answers

What is the primary function of the ameloblasts during maturation of enamel?

<p>Decrease in organelle content (B)</p> Signup and view all the answers

What changes occur to the stratum intermedium during the maturation stage?

<p>They transform from flat to spindle-shaped cells (C)</p> Signup and view all the answers

Which cellular component of ameloblasts increases in number during protein formation?

<p>Rough endoplasmic reticulum (D)</p> Signup and view all the answers

What happens to the ameloblasts after the maturation of enamel?

<p>They differentiate into the reduced enamel epithelium (A)</p> Signup and view all the answers

What is the role of desmolytic enzymes secreted by the reduced enamel epithelium?

<p>To facilitate tooth eruption by degenerating surrounding connective tissue (C)</p> Signup and view all the answers

What is necessary for a cell to effectively form and secrete proteins?

<p>An increase in the number of mitochondria (D)</p> Signup and view all the answers

Which phase follows after most of the enamel matrix is formed at the cusp tip?

<p>Maturative stage (C)</p> Signup and view all the answers

What are the features of an ameloblast's nucleus that indicate it is a protein forming and secreting cell?

<p>Large opened face nucleus and abundant cytoplasm (A)</p> Signup and view all the answers

What is the function of the distal terminal bar in ameloblasts?

<p>Serves as a barrier to prevent cell organelles from entering Tomes' process. (D)</p> Signup and view all the answers

During the formation of enamel, what structure is formed at the distal end of ameloblasts?

<p>Tomes' process (B)</p> Signup and view all the answers

What percentage of inorganic substance does mature enamel contain primarily after complete mineralization?

<p>96% (B)</p> Signup and view all the answers

Which of the following statements about enamel's permeability is correct for newly erupted teeth?

<p>Permeability occurs equally from the outer and pulpal sides. (B)</p> Signup and view all the answers

What is the composition of Tomes' process in ameloblasts?

<p>Secretory granules with no cell organelles. (C)</p> Signup and view all the answers

What occurs during the initial stage of mineralization in enamel formation?

<p>Partial mineralization of about 25-30%. (C)</p> Signup and view all the answers

Which of the following is NOT a factor contributing to the age changes of enamel?

<p>Color change (B)</p> Signup and view all the answers

What is the role of secretory granules in Tomes' process?

<p>They are secreted at a perpendicular angle to the membrane. (C)</p> Signup and view all the answers

Flashcards

Prism Sheath

The incomplete sheath surrounding enamel rods, resisting decalcification and staining darker than enamel.

Inter-Rod Substance

The material that cements enamel rods together, having a higher refractive index than the rods themselves.

Hunter-Shreger Bands

Optical bands on enamel resulting from changes in enamel rod direction. Appear as dark and light alternating bands.

Cross Striations

Transverse striations in enamel rods, marking daily enamel formation segments of approximately 4 microns.

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Incremental Lines of Retzius

Dark bands in enamel reflecting rhythmic enamel formation, showing periods of ameloblast rest and activity.

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Neonatal Lines

Brown striations in enamel, marking the separation between enamel formed before and after birth due to nutritional changes.

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Enamel Rod Direction Changes

The pattern of enamel rod direction affects visual appearance of Hunter-Shreger bands; important pattern is visible under reflected light, and not under transmitted light.

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Enamel composition

Enamel is primarily made up of inorganic substances (96%), mostly hydroxyapatite crystals, and a smaller amount of organic substances (4%) including proteins, carbohydrates, and water.

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Enamel color variation

Enamel color ranges from yellowish-white to grayish-white, depending on factors like translucency, calcification, and homogeneity.

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Enamel thickness

Enamel is thickest at the tips of teeth (cusps and incisal edges) and thins out towards the gumline (cervical region).

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Enamel hardness

Enamel is the hardest tissue in the human body, due to high calcification and crystal arrangement.

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Enamel brittleness

Despite its hardness, enamel can break easily if the underlying dentin (the softer tissue underneath) is damaged.

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Enamel permeability

Enamel functions like a semi-permeable membrane, allowing certain substances to pass through.

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Enamel rod direction (deciduous)

In baby teeth, enamel rods are vertical at the tips, become oblique, and horizontal near the gumline.

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Enamel rod direction (permanent)

In permanent teeth, enamel rods are similar to deciduous teeth (vertical-oblique), but near the gumline, they point towards the root.

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Enamel rod course

Enamel rods initially start straight near dentin but become wavy before becoming straight again towards the outer surface.

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Enamel rod diameter

The diameter of enamel rods increases as you move from the dentin-enamel junction towards the surface, in a 1:2 ratio.

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Enamel rod cross-section

Enamel rods can have hexagonal, oval, round, or 'fish scale' shapes in cross-sections.

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Enamel rod longitudinal section

In a longitudinal view, enamel rods resemble cylinders with striations.

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Enamel rod number variation

The number of enamel rods varies between teeth, from 5 million in some lower incisors to 12 million in upper first permanent molars.

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Enamel Quality (prenatal vs. postnatal)

Enamel formed before birth is generally better quality due to protected fetal conditions and consistent nutrition.

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Primary Enamel Cuticle

The final product secreted by ameloblasts; structurally similar to a basement membrane (viewed with electron microscopy).

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Enamel Lamellae

Hypocalcified enamel rods and inter-rod substance, resulting from stress during formation; appear as thin sheets extending from the enamel surface to the DEJ (dentinoenamel junction).

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Enamel Lamella Type A

Poorly calcified enamel rods/inter-rod substance, confined to the enamel.

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Enamel Lamella Type B

Pre-eruption enamel crack filled by adjacent tissues (epithelial cells or connective tissue).

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Enamel Lamella Type C

Post-eruption enamel crack, often containing saliva; can extend to dentin.

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Enamel Tuft

Hypocalcified enamel prisms/inter-prismatic substance, appearing as tufts in transverse sections, originating from the DEJ toward the enamel.

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Enamel Spindle

Odontoblastic process extending between inner dental epithelium cells before enamel formation; appears black in ground sections due to preparation process.

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Tomes' Process

A short, conical projection of the ameloblast cell, involved in enamel secretion.

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Distal Terminal Bar

Cytoplasmic condensation at the distal end of ameloblasts, separating Tomes' process from the cell body.

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Enamel Mineralization (Initial)

First stage of enamel creation, 25-30% of total enamel mineral is formed during matrix formation.

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Enamel Mineralization (Complete)

Second stage of enamel formation, mineralizing enamel to 96% inorganic substance

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Enamel Maturation

Enamel changes after tooth eruption that deposits ions from saliva, further increasing inorganic matter to 98%.

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Enamel Attrition

Physiological tooth wear, due to mastication.

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Enamel Permeability

The ability of substances to pass through enamel. Lower in older, older teeth.

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Enamel Hardness

Enamel's resistance to wear. Influenced by ionic exchange (e.g., nitrogen, fluorine).

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Structureless enamel

A thin, highly mineralized layer of enamel on the tooth surface, lacking enamel prisms. Typically found at the neck of the tooth.

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Perikymata

Tiny, wave-like grooves on the enamel surface, parallel to the cemento-enamel junction. They reflect incremental enamel growth lines.

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Rod ends

Concave depressions on the enamel surface, varying in depth and shape. Deeper towards the incisal/occlusal region

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Enamel Cracks

Fissure-like structures on enamel surfaces, extensions of enamel lamellae, showing a variable length.

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Primary Enamel Cuticle

A thin layer of organic material on the outer enamel surface, mostly formed by the reduced enamel epithelium as the tooth erupts.

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A fibrillar cementum

A type of cementum found along short portions of the cervical enamel area.

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Morphogenic stage (Ameloblasts)

Initial stage of ameloblast development, establishing the enamel-dentin junction and cell shape.

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Organizing stage (Ameloblasts)

Ameloblasts become taller and polarize, the cell free zone disappears, and dentin formation begins.

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Formative stage (Ameloblasts)

Ameloblasts continue to produce the enamel matrix as dentin formation is already well established

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Ameloblasts

Long cells that form enamel in teeth; they have a proximal nucleus and an increasing Golgi complex.

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Histodifferentiation

The process where cells change shape and function to become specialized.

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Reciprocal Induction

Cells influencing each other's development.

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Tomes' Process

The elongated projection of ameloblasts, important for enamel formation.

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Enamel Matrix Formation

The process of creating the initial enamel structure, which precedes enamel mineralization.

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Mineralization (Maturation)

The hardening of the enamel matrix after formation.

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Reduced Enamel Epithelium

A protective layer of epithelium that safeguards the enamel; forms 3-4 layers.

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Desmolytic Enzymes

Enzymes secreted to break down tissue, aiding tooth eruption.

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Maturative stage (Amelogenesis)

Stage of enamel formation marked by ameloblast shrinkage & organelle removal.

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Protective Stage

Ameloblasts become indistinguishable from the reduced enamel epithelium, completing enamel function.

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Desmolytic Stage

Epithelial cells facilitate tooth eruption through tissue breakdown.

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Study Notes

Enamel Composition

  • Enamel comprises 96% inorganic substances, primarily hydroxyapatite crystals (3Ca3(PO4)2Ca(OH)2).
  • 4% organic substances (1-2% protein and carbohydrates, 2-3% water).
  • Inorganic and organic components are equivalent by volume, despite weight disparity.

Enamel Physical Properties

  • Color varies from yellowish to grayish white, contingent on translucency, calcification, and homogeneity.
  • Thickness is substantial at incisal edges and cusp tips, thinning towards the cervical region (like a knife edge).
  • Enamel is the hardest calcified tissue due to high calcification and crystal orientation.
  • Despite hardness, enamel is brittle, especially when dentin integrity is compromised.
  • Enamel is a semi-permeable membrane.

Enamel Histological Structure

  • Enamel structure is comprised of enamel rods (enamel prisms), rod sheath, and inter-rod substance.
  • Enamel rod count varies from 5 million (lower central incisor) to 12 million (upper first permanent molar).
  • Rods are perpendicular to dentin surfaces and typically vertical on cusps/incisors, becoming oblique towards the occlusal surface then horizontal at the cervical line (ending abruptly).
  • Rod diameter increases from the dentino-enamel junction (DEJ) to enamel surface.

Hunter-Schreger Bands

  • An optical phenomenon due to alternating rod directions (wavy course).
  • Visible as dark and light bands in longitudinal ground sections, specifically on cervical two-thirds.
  • Visible bands are either dark (Diazones) or light (Parazones), reflecting or absorbing light.
  • Bands are not observable on incisal edges or cusp tips due to complex rod courses.

Incremental Lines of Enamel

  • Cross-striations: Rhythmic formations of enamel, about 4 microns in length, representing daily increments. Transverse bands appear in ground sections, identified as dark striations.
  • Incremental lines of Retzius: Dark bands reflecting enamel formation rhythms, beginning at the D.E.J., extending upward. These bands do not reach the enamel surface until the cervical region. Seen as transverse grooves in cross-section.

Neonatal Lines

  • A part of the Retzius lines; a brown stria found in deciduous and first permanent molars.
  • A demarcation line separating enamel formed before birth from enamel formed after birth.
  • Influenced by changes in nutrition and environmental conditions during birth.

Enamel Lamellae

  • Thin sheet-like structures extending from the enamel surface, occasionally reaching the dentin-enamel junction.
  • Can be longitudinal or transverse.
  • May manifest as cracks, filled with epithelial cells or connective tissues, and cause degradation of the dentin-enamel junction.
  • Three types: A (true lamella - poorly calcified enamel rod), B (a crack), and C (filled with organic matter from saliva).

Enamel Tufts

  • Hypocalcified enamel structures appearing as tufts of grass.
  • Mostly visible in transverse sections.
  • Located at the dentino-enamel junction and extend to about a third of the enamel's thickness.
  • Formation arises from the wavy course of enamel rods.

Enamel Spindles

  • Processes of odontoblasts extending between cells of the inner enamel epithelium, visible as rounded or pointed structures in ground sections.
  • Appear as black spots due to processing methods.
  • Occur in great numbers near cusp tips and incisal edges.

Surface Structure of Enamel

  • Structureless Enamel: The outer layer, highly mineralized, up to 30 microns thick.
  • Perikymata: Groove-like structures reflecting Retzius lines, running parallel to the cemento-enamel junction.

Rod Ends

  • Concave depressions in enamel, varying in depth and form.
  • Generally more evident in the cervical regions of the enamel.

Cracks

  • Fissures in enamel that can extend from the surface's outer edge to varying distances.
  • May disappear with thorough decalcification.

Primary Enamel Cuticle

  • Final product secreted by ameloblasts.
  • Acting as a basement membrane by electron microscope observation.

Fibrillar Cementum

  • Type of cementum forming a short structure, usually on the cervix, due to the degeneration of the reduced dental epithelium before eruption.

Life Cycle of Ameloblasts

  • Morphogenic Stage: Inner enamel epithelium, differentiating into ameloblasts.
    • Ameloblasts have large, oval nuclei positioned near the cell base.
  • Organizing Stage: Ameloblasts enlarge and organize, changing their shape and orientation.
  • Formative Stage: Ameloblasts finalize dentin and enamel formation.
  • Maturative Stage: Ameloblasts reduce in size, decrease in organelles, and produce enamel matrix.
  • Protective Stage: Ameloblasts differentiate further, with the enamel organ evolving into a protective stratified epithelium layer.

Other information

  • Enamel continues to mature after tooth eruption with mineral deposition, increasing in inorganic matter (up to 98%).
  • Age Changes: Attrition, the normal wear and tear, affects enamel surfaces (incisal, occlusal, and proximal).
  • Permeability: Enamel is permeable in newly erupted teeth, but this permeability may decrease or disappear in older teeth, more noticeably from the oral side.
  • Hardness: Ionic exchange with substances like nitrogen and fluorine in enamel, enhances resistance to decay.
  • Color: Enamel darkens over time.

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