Edexcel Biology GCSE - Key Concepts in Biology

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Questions and Answers

What is the primary function of egg cells?

  • To accept a single sperm cell and develop into an embryo. (correct)
  • To transport nutrients to the developing embryo.
  • To secrete hormones for embryo development.
  • To provide structural support to the embryo.

How do ciliated epithelial cells help defend the body against illness?

  • By engulfing and digesting harmful pathogens.
  • By producing antibodies that neutralize bacteria.
  • By absorbing nutrients from digested food.
  • By wafting bacteria trapped by mucus down to the stomach. (correct)

What structural adaptation do root hair cells have to maximize water uptake?

  • A small vacuole for efficient water storage.
  • A thick cell wall to prevent damage.
  • A layered membrane to filter contaminants.
  • A large surface area due to root hairs. (correct)

What causes xylem cells to become hollow tubes?

<p>The deposition of lignin which leads to cell death. (C)</p> Signup and view all the answers

How do root hair cells transport mineral ions from the soil?

<p>By active transport which requires energy. (C)</p> Signup and view all the answers

What role does mitochondria play in egg cells?

<p>They supply energy for the developing embryo. (C)</p> Signup and view all the answers

What is the function of cilia on ciliated epithelial cells?

<p>To waft bacteria trapped by mucus toward the stomach. (C)</p> Signup and view all the answers

What characteristic of xylem cells helps them withstand the pressure from moving water?

<p>The lignin deposited in spirals enhances their strength. (C)</p> Signup and view all the answers

Which reagent is used to test for reducing sugars?

<p>Benedict’s solution (A)</p> Signup and view all the answers

What method is used to identify the presence of reducing sugars?

<p>Heat the sample with Benedict's solution in a water bath (C)</p> Signup and view all the answers

Which enzyme is primarily involved in building carbohydrates, lipids, and proteins?

<p>Complex enzymes (A)</p> Signup and view all the answers

What observation indicates that reducing sugars are present after testing?

<p>Change to reddish-brown (B)</p> Signup and view all the answers

What happens to glucose during the process of respiration?

<p>It is used to generate energy (A)</p> Signup and view all the answers

What must occur before the presence of starch can be confirmed with iodine solution?

<p>The sample must be boiled (D)</p> Signup and view all the answers

Which nutrient do enzymes specifically build, aside from carbohydrates and lipids?

<p>Proteins (C)</p> Signup and view all the answers

What indicates the presence of proteins when conducting a Biuret test?

<p>Change from blue to violet (C)</p> Signup and view all the answers

Which substances are added to test for lipids in the Emulsion Test?

<p>Ethanol and deionised water (D)</p> Signup and view all the answers

In calorimetry, what is the first step when measuring the energy in food?

<p>Record the starting temperature of cold water (C)</p> Signup and view all the answers

What is the purpose of using a control in the Biuret and Emulsion tests?

<p>To establish a baseline for positive and negative results (A)</p> Signup and view all the answers

What color change is observed if lipids are present in the Emulsion Test?

<p>Formation of a white emulsion layer (A)</p> Signup and view all the answers

What is the correct procedure after adding 2cm​3 ​of ethanol to the food sample in the Emulsion Test?

<p>Shake thoroughly and add water (B)</p> Signup and view all the answers

At what angle should the test tube be held during the calorimetry experiment?

<p>45 degrees (D)</p> Signup and view all the answers

Which of the following is a positive control for the Biuret test?

<p>Egg white (B)</p> Signup and view all the answers

What is the primary function of phloem cells in plants?

<p>To carry the products of photosynthesis to all parts of the plants (A)</p> Signup and view all the answers

Which type of microscope would be best for viewing the internal structures of organelles like mitochondria?

<p>Transmission electron microscope (B)</p> Signup and view all the answers

What does the resolving power (RP) of a microscope indicate?

<p>The ability to distinguish between two points (B)</p> Signup and view all the answers

Which microscope type requires the use of electrons to form an image?

<p>Scanning electron microscope (B)</p> Signup and view all the answers

What major advancement does the electron microscope provide over the light microscope?

<p>Higher magnification and resolving power (D)</p> Signup and view all the answers

Which microscope is specifically known for producing 3D images?

<p>Scanning electron microscope (C)</p> Signup and view all the answers

Why are electron microscopes particularly useful for identifying viruses?

<p>They can magnify specimens to 2,000,000x (D)</p> Signup and view all the answers

What is a significant characteristic of light microscopes?

<p>They can view living cells without preparation (A)</p> Signup and view all the answers

What effect does a change in pH have on proteins?

<p>It affects the amino acid chain structure. (A)</p> Signup and view all the answers

What happens to the active site of an enzyme when it is denatured?

<p>It changes shape and can no longer bind with the substrate. (D)</p> Signup and view all the answers

What is the saturation point in enzyme activity?

<p>The concentration beyond which increasing substrate does not increase reaction rate. (C)</p> Signup and view all the answers

What color indicates the presence of starch when iodine is used?

<p>Blue-black (A)</p> Signup and view all the answers

Which enzyme is being tested for activity in this practical?

<p>Amylase (D)</p> Signup and view all the answers

How is the optimal pH for amylase activity determined in this experiment?

<p>By timing how long it takes for starch to be broken down. (C)</p> Signup and view all the answers

What materials are used to test the effect of pH on enzyme activity?

<p>Amylase solution, starch solution, iodine, and buffer solutions. (D)</p> Signup and view all the answers

What is the primary role of amylase in the experiment?

<p>To convert starch into simple sugars. (B)</p> Signup and view all the answers

What does the term 'enzyme specificity' refer to?

<p>The unique shape of an enzyme's active site that allows only specific substrates to bind. (C)</p> Signup and view all the answers

What happens to an enzyme when the temperature exceeds its optimum level?

<p>The enzyme becomes denatured and its active site changes shape. (B)</p> Signup and view all the answers

What is the relationship described by the Lock and Key Hypothesis?

<p>The shape of the substrate must match the active site of the enzyme for binding to occur. (D)</p> Signup and view all the answers

Which of the following statements about the optimum conditions for enzyme activity is true?

<p>Most enzymes have an optimum pH of around 7, but some require a lower pH. (A)</p> Signup and view all the answers

What defines the process of denaturation in enzymes?

<p>The alteration of the enzyme's structure, preventing it from functionally binding to the substrate. (A)</p> Signup and view all the answers

How does substrate concentration affect enzyme activity up to the point of saturation?

<p>Higher substrate concentrations lead to a constant enzyme activity level. (A)</p> Signup and view all the answers

Why are enzymes considered biological catalysts?

<p>They remain unchanged after the reaction and increase the rate of reaction. (C)</p> Signup and view all the answers

Which factor is most critical for the binding of a substrate to an enzyme?

<p>The matching shapes of the substrate and the enzyme's active site. (C)</p> Signup and view all the answers

Flashcards

Egg cell function

Egg cells are designed to accept one sperm and develop into an embryo.

Egg cell membrane

The egg cell membrane only accepts one sperm. It becomes impenetrable after fertilization.

Ciliated epithelial cells

Move bacteria to the stomach where they're digested.

Root hair cells

Absorb water and minerals from soil. Specialized for water absorption.

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Root hair cell surface area

They have a large surface area to absorb more water and minerals.

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Xylem cell function

Transport water and minerals up the plant.

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Lignin in Xylem

Hardens xylem cells, making them strong enough to transport water.

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Xylem cell structure

Xylem cells are hollow, joined end-to-end, and contain lignin.

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Phloem cells function

Phloem cells are specialized to transport food (products of photosynthesis) throughout the plant.

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Sieve plates

Structures formed by the breakdown of cell walls in phloem cells, allowing substance movement between cells.

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Phloem cell energy source

The energy needed for phloem cell function is supplied by mitochondria in companion cells.

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Light microscope function

A light microscope enlarges images, allowing viewing of tissues, cells, and large sub-cellular structures.

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Electron microscope's advantage

Electron microscopes provide higher magnification and resolution to view sub-cellular structures like ribosomes and viruses in great detail.

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Electron microscope types

Electron microscopes come in scanning (3D images) and transmission (2D images) varieties.

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Electron microscope application

Electron microscopes are used to study viruses and proteins in far greater detail than light microscopes.

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Electron microscope resolving power

Electron microscopes have a resolving power much higher than light microscopes, allowing for greater detail.

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Enzymes: What are they?

Enzymes are biological catalysts, meaning they speed up reactions without being used up. They are essential for many biological processes, including breaking down large molecules and joining small ones.

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Active Site

The active site is the unique, 3D shaped region on an enzyme where the substrate binds.

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Lock and Key Hypothesis

This hypothesis explains how enzymes work. The shape of the substrate is complementary to the active site, allowing them to bind and form an enzyme-substrate complex.

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Enzyme Specificity

Enzymes have specificity, meaning they can only catalyze reactions for substrates that have a complementary shape to their active site.

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Optimum Temperature

Each enzyme has an optimum temperature where it functions best. Above or below this temperature, the enzyme's activity decreases, potentially leading to denaturation.

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Denaturation

When an enzyme loses its shape due to extreme temperatures (above optimum) or pH changes, it can't bind to its substrate and becomes inactive.

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Optimum pH for Enzymes

Most enzymes have an optimum pH of around 7 (neutral). However, some enzymes, like those in the stomach, function best in acidic conditions.

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Substrate Concentration

Enzyme activity is also affected by the concentration of the substrate. As the substrate concentration increases, the rate of reaction also increases up to a point, after which the enzyme becomes saturated.

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Carbohydrate, lipid, and protein synthesis

The process of building new carbohydrates, lipids, and proteins from simpler molecules.

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Enzymes in biosynthesis

Special enzymes are required to accelerate the reactions that build carbohydrates, lipids, and proteins.

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Food tests

Specific tests used to identify the presence of different nutrients like starch, sugars, protein, and lipids in food samples.

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Starch test

A test using iodine solution to detect starch in a food sample. A color change from orange to blue-black indicates the presence of starch.

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Reducing sugars test

A test using Benedict's solution to detect reducing sugars in a food sample. A color change to reddish-brown indicates the presence of reducing sugars.

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Benedict's solution

A blue solution used in the reducing sugars test to detect the presence of sugars that can reduce copper ions.

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Boiling water bath

A method used to heat the sample and Benedict's solution in the reducing sugars test, providing the necessary heat for the reaction.

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Investigating Macronutrients

A practical experiment to identify the presence of different macronutrients (carbohydrates, lipids, and proteins) in food samples using specific tests.

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Enzyme denaturation

When an enzyme's shape is altered by factors like extreme pH, making it unable to bind to its substrate. This is irreversible and results in loss of enzyme activity.

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Enzyme's Active Site

The specific region on an enzyme where the substrate binds, allowing the chemical reaction to occur.

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Substrate Concentration Impact

The rate of an enzymatic reaction increases as the substrate concentration increases, but only up to a certain point.

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Saturation Point

The point where adding more substrate will not increase the reaction rate because all active sites of the enzyme are occupied.

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Amylase

An enzyme that breaks down carbohydrates like starch into simpler sugars like maltose.

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Iodine Test for Starch

Iodine solution turns blue-black in the presence of starch.

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Optimal pH

The specific pH value that allows an enzyme to function at its maximum rate.

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Estimating Amylase's Optimal pH

By testing amylase activity at different pH levels, we can determine the pH value at which it functions best.

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Biuret Test

A chemical test to detect the presence of proteins in a sample. It involves adding potassium hydroxide and copper sulfate solution, which turns the solution violet if protein is present.

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Emulsion Test

A test to identify the presence of lipids (fats and oils) in a sample. Ethanol is added to the sample, followed by water, leading to a milky white emulsion layer forming on top if lipids are present.

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Control in Experiments

A standard sample used as a reference in an experiment. Controls help determine whether the results are due to the variable being tested or other factors.

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Calorimetry

A technique used to measure the energy absorbed or released during a chemical reaction, such as burning food to determine its caloric content.

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Calorimetry Steps

Calorimetry involves 1) heating a known volume of water, 2) recording its initial temperature, 3) burning a food sample to heat the water, and 4) recording the final temperature to calculate the calories in the food.

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Positive Control

A control sample that is known to contain the variable being tested, ensuring the test is working correctly.

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Negative Control

A control sample that does not contain the variable being tested, ensuring the test is not giving false positives due to other factors.

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Study Notes

Edexcel Biology GCSE - Key Concepts in Biology

  • Topic 1.1: Eukaryotic and Prokaryotic Cell Functions

    • Living things are made of cells (prokaryotic or eukaryotic)
    • Animal and plant cells are eukaryotic
      • Cell membrane
      • Cytoplasm
      • Nucleus containing DNA
    • Bacterial cells are prokaryotic
      • Cell wall
      • Cell membrane
      • Cytoplasm
      • Single circular strand of DNA and plasmids
    • Organelles have specific functions within cells
  • Topic 1.2: Specialised Cells and Their Functions

    • Cells specialise through differentiation, gaining new sub-cellular structures for their role
    • Some cells differentiate once early in life, others throughout their lifetime (stem cells)
    • Animal examples:
      • Sperm cells: streamlined head, long tail, many mitochondria, acrosome
      • Egg cells: surrounded by a membrane, many mitochondria, large size
      • Ciliated epithelial cells: long hair-like cilia to waft mucus
    • Plant examples:
      • Root hair cells: large surface area, large permanent vacuole, mitochondria
      • Xylem cells: hollow, lignin for strength, transport water and minerals
  • Topic 1.3: Microscopy

    • Cells too small to see without microscopes
    • Light microscopes:
      • Two lenses
      • Illuminated from underneath
      • Maximum magnification about 2000x
      • Resolving power of 200nm
    • Electron microscopes (1930s):
      • Use electrons instead of light
      • Higher magnification (up to 2,000,000x)
      • Higher resolving power (10nm for SEM, 0.2nm for TEM)
      • Used to view very small structures, including viruses
  • Topic 1.4: Size, Scale, and Estimations

    • Calculations involving magnification and size of specimens
    • Standard form for very large and very small numbers, where a number is multiplied by a power of 10 (e.g. 1.5 x 10-5)
    • Orders of magnitude to describe how much bigger or smaller one thing is than another. (e.g., 101, 103)
    • Estimations useful when exact numbers are not known (e.g., counting plants in a field)
  • Topic 1.5: Units and Standard Form

    • Importance of using correct units in calculations.
    • Using prefixes (e.g., centi, milli, micro, nano) to denote multiples of units
  • Topic 1.6: Core Practical: Investigating Biological Specimens

    • This involves using a light microscope to view biological specimens
    • Steps for using a light microscope:
      • Place slide on the stage
      • Use the objective lens with the lowest magnification initially
      • Adjust the focus wheel
      • Increase magnification and refocus.
  • Topic 1.7, 1.8, and 1.9: Enzymes: Mechanisms, Denaturation, and Factors Affecting Enzyme Activity

    • Enzymes are biological catalysts
    • Enzymes are proteins with specific 3D shapes. Active site corresponds with substrate.
    • Enzymes speed up reactions without being consumed by reacting with substrates.
    • Optimum pH is important for enzymes: Enzymes have a specific pH level at which they work most efficiently.
    • Optimum temperature is also important: The rate of enzyme activity increases with temperature until it reaches a maximum point.
    • Enzymes denature when temperature or pH is too extreme (changes the active site shape).
  • Topic 1.10: Core Practical: Effect of pH on Enzyme Activity

    • Investigating how pH affects the rate of enzyme activity.
    • Amylase as an example enzyme.
    • Iodine solution detects the presence of starch.
    • Optimal pH for the enzyme (e.g. in amylase experiments)
  • Topic 1.11: Rate Calculations for Enzyme Activity

    • Rate = Change / Time (calculating the rate of enzyme-catalyzed reactions).
  • Topic 1.12: Enzymes as Biological Catalysts

    • Different enzymes performing functions (carbohydrases, proteases, lipases)
    • Enzymes convert different types of biomolecules (carbohydrates, proteins, or lipids) into specific products (e.g. simple sugars, amino acids, fatty acids).
  • Topic 1.13: Core Practical: Investigating Macronutrients

    • Tests for the presence of: starch, reducing sugars, proteins, lipids.
    • Specific reagents and methods exist in food testing, leading to specific color changes to indicate the presence of a particular nutrient.
  • Topic 1.14: Core Practical: Calorimetry

    • Measuring the energy in food
    • Using a water bath to calculate heat transferred to water.
  • Topic 1.15: Transport in and Out of Cells

    • Passive transport processes (diffusion, osmosis) do not require energy. Move from high to low concentration.
    • Active transport requires energy (ATP). Moves substances against the concentration gradient.
  • Topic 1.16: Core Practical: Osmosis in Potatoes

    • Investigating the movement of water across cell membranes
    • Determining % change in mass in potato cylinders exposed to different concentrations of sucrose solutions.
  • Topic 1.17: Percentage Gain and Loss of Mass

    • Calculating % change in mass related to osmosis or other experiments.
    • Formula: % Change = (change in mass/ initial mass) x 100
  • Explaining the experiment with controls, independent and dependent variables.

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